Human CCR7 Alexa Fluor® 700-conjugated Antibody

Clone 150503 was used by HLDA to establish CD designation.
(2 citations)   
  • Species Reactivity
  • Specificity
    Detects human CCR7.
  • Source
    Monoclonal Mouse IgG2A Clone # 150503
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Human CCR7 transfectants
    Accession # AAA58615
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 700
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CCR7 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD4 PE‑conjugated Monoclonal Antibody (Catalog # FAB3791P) and either (A) Mouse Anti-Human CCR7 Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB197N) or (B) Mouse IgG2A Alexa Fluor 700 Isotype Control (Catalog # IC003N). View our protocol for Staining Membrane-associated Proteins.
Detection of CCR7 in HEK293 Human Cell Line Transfected with Human CCR7 and eGFP by Flow Cytometry. The specificity of Mouse Anti-Human CCR7 Clone 150503 was demonstrated by its ability to react with (A) HEK293 human embryonic kidney cell line transfected with human CCR7 and not react with (B) irrelevant HEK293 transfectants. Antibody binding was monitored using Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CCR7

CCR7 is a 7 transmembrane G protein coupled chemokine receptor. CCR7 is expressed on T cells and mature dendritic cells and transduces chemotactic signals in response to CCL19 and CCL21. Human CCR7 shares 87% amino acid sequence identity with mouse CCR7.

  • Entrez Gene IDs:
    1236 (Human); 12775 (Mouse); 287673 (Rat)
  • Alternate Names:
    BLR2C-C chemokine receptor type 7; C-C CKR-7; CC-CKR-7; CCR7; CCR-7; CD197 antigen; CD197; CDw197CC chemokine receptor 7; chemokine (C-C motif) receptor 7; CMKBR7chemokine (C-C) receptor 7; EBI1EBV-induced G protein-coupled receptor 1; EBV-induced G-protein coupled receptor 1; Epstein-Barr virus induced gene 1; Epstein-Barr virus induced G-protein coupled receptor; Epstein-Barr virus-induced G-protein coupled receptor 1; EVI1; lymphocyte-specific G protein-coupled peptide receptor; MIP-3 beta receptor
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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Sample Type
  1. Hypercytotoxicity and rapid loss of NKp44+ innate lymphoid cells during acute SIV infection.
    Authors: Li H, Richert-Spuhler L, Evans T, Gillis J, Connole M, Estes J, Keele B, Klatt N, Reeves R
    PLoS Pathog, 2014;10(12):e1004551.
    Species: Rhesus Macaque
    Sample Type: Whole Cells
    Application: Flow
  2. Phenotypic complexity of T regulatory subsets in patients with B-chronic lymphocytic leukemia.
    Authors: Biancotto A, Dagur PK, Fuchs JC, Wiestner A, Bagwell CB, McCoy JP
    Mod. Pathol., 2012;25(2):246-59.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A Alexa Fluor® 700-conjugated Antibody

Ctrl IC003N  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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