Human CCR7 PE-conjugated Antibody

Clone 150503 was used by HLDA to establish CD designation.
(46 citations)
(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human CCR7.
  • Source
    Monoclonal Mouse IgG2A Clone # 150503
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Human CCR7 transfectants
    Met1-Pro378
    Accession # AAA58615
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CCR7 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD4 APC‑conjugated Monoclonal Antibody (Catalog # FAB3791A) and either (A) Mouse Anti-Human CCR7 PE‑conjugated Monoclonal Antibody (Catalog # FAB197P) or (B) Mouse IgG2A Phycoerythrin Isotype Control (Catalog # IC003P). View our protocol for Staining Membrane-associated Proteins.
Detection of CCR7 in HEK293 Human Cell Line Transfected with Human CCR7 and eGFP by Flow Cytometry. The specificity of Mouse Anti-Human CCR7 Clone 150503 was demonstrated by its ability to react with (A) HEK293 human embryonic kidney cell line transfected with human CCR7 and not react with (B) irrelevant HEK293 transfectants. Antibody binding was monitored using Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CCR7

CCR7 is a 7 transmembrane G protein coupled chemokine receptor. CCR7 is expressed on T cells and mature dendritic cells and transduces chemotactic signals in response to CCL19 and CCL21. Human CCR7 shares 87% amino acid sequence identity with mouse CCR7.

  • Entrez Gene IDs:
    1236 (Human); 12775 (Mouse); 287673 (Rat)
  • Alternate Names:
    BLR2C-C chemokine receptor type 7; C-C CKR-7; CC-CKR-7; CCR7; CCR-7; CD197 antigen; CD197; CDw197CC chemokine receptor 7; chemokine (C-C motif) receptor 7; CMKBR7chemokine (C-C) receptor 7; EBI1EBV-induced G protein-coupled receptor 1; EBV-induced G-protein coupled receptor 1; Epstein-Barr virus induced gene 1; Epstein-Barr virus induced G-protein coupled receptor; Epstein-Barr virus-induced G-protein coupled receptor 1; EVI1; lymphocyte-specific G protein-coupled peptide receptor; MIP-3 beta receptor
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

46 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Pertussis circulation has increased T-cell immunity during childhood more than a second acellular booster vaccination in Dutch children 9 years of age.
    Authors: Schure R, de Rond L, Ozturk K, Hendrikx L, Sanders E, Berbers G, Buisman A
    PLoS ONE, 2102;7(7):e41928.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Impact of Vaccination on Distribution of T Cell Subsets in Antiretroviral-Treated HIV-Infected Children
    Authors: P Thitilertd, L Khowawiset, P Ammaranond, P Poungpairo, V Tantithavo, N Onlamoon
    Dis. Markers, 2017;2017(0):5729639.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. E. coli promotes human V?9V?2?T cell transition from cytokine-producing bactericidal effectors to professional phagocytic killers in a TCR-dependent manner
    Authors: M Barisa, AM Kramer, Y Majani, D Moulding, L Saraiva, M Bajaj-Elli, J Anderson, K Gustafsson
    Sci Rep, 2017;7(1):2805.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. Effective Cytotoxic T Lymphocyte Targeting of Persistent HIV-1 during Antiretroviral Therapy Requires Priming of Naive CD8+ T Cells
    Authors: Kellie N Smith
    MBio, 2016;7(3):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. Cognate HLA absence in trans diminishes human NK cell education
    J Clin Invest, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  6. Role of Occult and Post-acute Phase Replication in Protective Immunity Induced with a Novel Live Attenuated SIV Vaccine
    PLoS Pathog, 2016;12(12):e1006083.
    Species: Primate
    Sample Type: Whole Cells
    Application: Flow Cytometery
  7. Functional capacities of human IgM memory B cells in early inflammatory responses and secondary germinal center reactions.
    Authors: Seifert M, Przekopowitz M, Taudien S, Lollies A, Ronge V, Drees B, Lindemann M, Hillen U, Engler H, Singer B, Kuppers R
    Proc Natl Acad Sci U S A, 2015;112(6):E546-55.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  8. Different maturation cocktails provide dendritic cells with different chemoattractive properties.
    Authors: Massa C, Thomas C, Wang E, Marincola F, Seliger B
    J Transl Med, 2015;13(1):175.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  9. Effector Vgamma9Vdelta2 T cells dominate the human fetal gammadelta T-cell repertoire.
    Authors: Dimova T, Brouwer M, Gosselin F, Tassignon J, Leo O, Donner C, Marchant A, Vermijlen D
    Proc Natl Acad Sci U S A, 2015;112(6):E556-65.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  10. Characterization of CD8+ T cell differentiation following SIVDeltanef vaccination by transcription factor expression profiling.
    Authors: Billingsley J, Rajakumar P, Connole M, Salisch N, Adnan S, Kuzmichev Y, Hong H, Reeves R, Kang H, Li W, Li Q, Haase A, Johnson R
    PLoS Pathog, 2015;11(3):e1004740.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Type: Whole Cells
    Application: Flow
  11. Antigen-specific activation and cytokine-facilitated expansion of naive, human CD8+ T cells.
    Authors: Wolfl M, Greenberg P
    Nat Protoc, 2014;9(4):950-66.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  12. Chemokines as novel and versatile reagents for flow cytometry and cell sorting.
    Authors: Le Brocq M, Fraser A, Cotton G, Woznica K, McCulloch C, Hewit K, McKimmie C, Nibbs R, Campbell J, Graham G
    J Immunol, 2014;192(12):6120-30.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  13. IL-2 phosphorylates STAT5 to drive IFN-gamma production and activation of human dendritic cells.
    Authors: Herr F, Lemoine R, Gouilleux F, Meley D, Kazma I, Heraud A, Velge-Roussel F, Baron C, Lebranchu Y
    J Immunol, 2014;192(12):5660-70.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  14. Treatment with chemotherapy and dendritic cells pulsed with multiple Wilms' tumor 1 (WT1)-specific MHC class I/II-restricted epitopes for pancreatic cancer.
    Authors: Koido S, Homma S, Okamoto M, Takakura K, Mori M, Yoshizaki S, Tsukinaga S, Odahara S, Koyama S, Imazu H, Uchiyama K, Kajihara M, Arakawa H, Misawa T, Toyama Y, Yanagisawa S, Ikegami M, Kan S, Hayashi K, Komita H, Kamata Y, Ito M, Ishidao T, Yusa S, Shimodaira S, Gong J, Sugiyama H, Ohkusa T, Tajiri H
    Clin Cancer Res, 2014;20(16):4228-39.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  15. IL-27 in human secondary lymphoid organs attracts myeloid dendritic cells and impairs HLA class I-restricted antigen presentation.
    Authors: Morandi, Fabio, Di Carlo, Emma, Ferrone, Soldano, Petretto, Andrea, Pistoia, Vito, Airoldi, Irma
    J Immunol, 2014;192(6):2634-42.
  16. A thymic stromal lymphopoietin-responsive dendritic cell subset mediates allergic responses in the upper airway mucosa.
    Authors: Melum G, Farkas L, Scheel C, Van Dieren B, Gran E, Liu Y, Johansen F, Jahnsen F, Baekkevold E
    J Allergy Clin Immunol, 2014;134(3):613-621.e7.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  17. Enlarged memory T-cell pool and enhanced Th1-type responses in chronic myeloid leukemia patients who have successfully discontinued IFN-alpha monotherapy.
    Authors: Ilander M, Kreutzman A, Rohon P, Melo T, Faber E, Porkka K, Vakkila J, Mustjoki S
    PLoS ONE, 2014;9(1):e87794.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  18. Manipulating the expression of chemokine receptors enhances delivery and activity of cytokine-induced killer cells.
    Authors: Zou Y, Li F, Hou W, Sampath P, Zhang Y, Thorne S
    Br J Cancer, 2014;110(8):1992-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  19. Dasatinib promotes Th1-type responses in granzyme B expressing T-cells.
    Authors: Kreutzman A, Ilander M, Porkka K, Vakkila J, Mustjoki S
    Oncoimmunology, 2014;3(0):e28925.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  20. Human regulatory T cells lack the cyclophosphamide-extruding transporter ABCB1 and are more susceptible to cyclophosphamide-induced apoptosis.
    Authors: Dimeloe S, Frick C, Fischer M, Gubser P, Razik L, Bantug G, Ravon M, Langenkamp A, Hess C
    Eur J Immunol, 2014;44(12):3614-20.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  21. Conversion of peripheral blood NK cells to a decidual NK-like phenotype by a cocktail of defined factors.
    Authors: Cerdeira A, Rajakumar A, Royle C, Lo A, Husain Z, Thadhani R, Sukhatme V, Karumanchi S, Kopcow H
    J Immunol, 2013;190(8):3939-48.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  22. The road to memory: an early rest for the long journey.
    Authors: Ho, Liam Poc, Yit, Pui San, Ng, Lee Hui, Linn, Yeh Chin, Zhao, Yi, Sun, Li, Ling, Khoon Li, Chai Koh, Mickey B, Monica Shih, Meng-Chu, Li, Shang, Wang, Xue Ying, Tien, Sim Leng, Goh, Yeow Tee
    J Immunol, 2013;191(11):5603-14.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  23. Biomarkers on melanoma patient T cells associated with ipilimumab treatment.
    Authors: Wang W, Yu D, Sarnaik A, Yu B, Hall M, Morelli D, Zhang Y, Zhao X, Weber J
    J Transl Med, 2012;10(0):146.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  24. Thymoquinone inhibits the CXCL12-induced chemotaxis of multiple myeloma cells and increases their susceptibility to Fas-mediated apoptosis.
    Authors: Badr G, Lefevre EA, Mohany M
    PLoS ONE, 2011;6(9):e23741.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  25. CD56highCD16-CD62L- NK cells accumulate in allergic contact dermatitis and contribute to the expression of allergic responses.
    Authors: Carbone T, Nasorri F, Pennino D
    J. Immunol., 2010;184(2):1102-10.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  26. Distinct roles for CCR4 and CXCR3 in the recruitment and positioning of regulatory T cells in the inflamed human liver.
    Authors: Oo YH, Weston CJ, Lalor PF, Curbishley SM, Withers DR, Reynolds GM, Shetty S, Harki J, Shaw JC, Eksteen B, Hubscher SG, Walker LS, Adams DH
    J. Immunol., 2010;184(6):2886-98.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  27. Production of interleukin 22 but not interleukin 17 by a subset of human skin-homing memory T cells.
    Authors: Duhen T, Geiger R, Jarrossay D, Lanzavecchia A, Sallusto F
    Nat. Immunol., 2009;10(8):857-63.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  28. Chemokine responsiveness of CD4+ CD25+ regulatory and CD4+ CD25- T cells from atopic and nonatopic donors.
    Authors: Ahern D, Lloyd CM, Robinson DS
    Allergy, 2009;64(8):1121-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  29. Homeostatic chemokines CCL19 and CCL21 promote inflammation in human immunodeficiency virus-infected patients with ongoing viral replication.
    Authors: Damas JK, Landro L, Fevang B, Heggelund L, Tjonnfjord GE, Floisand Y, Halvorsen B, Froland SS, Aukrust P
    Clin. Exp. Immunol., 2009;157(3):400-7.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  30. Expression of CXC and CC type of chemokines and its receptors in tuberculous and non-tuberculous effusions.
    Authors: Pokkali S, Das SD, R L
    Cytokine, 2008;41(3):307-14.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  31. IL-12-impaired and IL-12-secreting dendritic cells produce IL-23 upon CD154 restimulation.
    Authors: Jasny E, Eisenblatter M, Matz-Rensing K, Tenner-Racz K, Tenbusch M, Schrod A, Stahl-Hennig C, Moos V, Schneider T, Racz P, Uberla K, Kaup FJ, Ignatius R
    J. Immunol., 2008;180(10):6629-39.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Type: Whole Cells
    Application: Flow
  32. BAFF enhances chemotaxis of primary human B cells: a particular synergy between BAFF and CXCL13 on memory B cells.
    Authors: Badr G, Borhis G, Lefevre EA, Chaoul N, Deshayes F, Dessirier V, Lapree G, Tsapis A, Richard Y
    Blood, 2008;111(5):2744-54.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  33. CD43, but not P-selectin glycoprotein ligand-1, functions as an E-selectin counter-receptor in human pre-B-cell leukemia NALL-1.
    Authors: Nonomura C, Kikuchi J, Kiyokawa N, Ozaki H, Mitsunaga K, Ando H, Kanamori A, Kannagi R, Fujimoto J, Muroi K, Furukawa Y, Nakamura M
    Cancer Res., 2008;68(3):790-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  34. Lactoferrin, a major defense protein of innate immunity, is a novel maturation factor for human dendritic cells.
    Authors: Spadaro M, Caorsi C, Ceruti P, Varadhachary A, Forni G, Pericle F, Giovarelli M
    FASEB J., 2008;22(8):2747-57.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  35. Dissociation of its opposing immunologic effects is critical for the optimization of antitumor CD8+ T-cell responses induced by interleukin 21.
    Authors: Ansen S, Butler MO, Berezovskaya A, Murray AP, Stevenson K, Nadler LM, Hirano N
    Clin. Cancer Res., 2008;14(19):6125-36.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  36. Evidence for HIV-associated B cell exhaustion in a dysfunctional memory B cell compartment in HIV-infected viremic individuals.
    Authors: Moir S, Ho J, Malaspina A, Wang W, DiPoto AC, O'Shea MA, Roby G, Kottilil S, Arthos J, Proschan MA, Chun TW, Fauci AS
    J. Exp. Med., 2008;205(8):1797-805.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  37. Interleukins 1beta and 6 but not transforming growth factor-beta are essential for the differentiation of interleukin 17-producing human T helper cells.
    Authors: Acosta-Rodriguez EV, Napolitani G, Lanzavecchia A, Sallusto F
    Nat. Immunol., 2007;8(9):942-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  38. Ability of herpes simplex virus vectors to boost immune responses to DNA vectors and to protect against challenge by simian immunodeficiency virus.
    Authors: Kaur A, Sanford HB, Garry D, Lang S, Klumpp SA, Watanabe D, Bronson RT, Lifson JD, Rosati M, Pavlakis GN, Felber BK, Knipe DM, Desrosiers RC
    Virology, 2007;357(2):199-214.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Type: Whole Cells
    Application: Flow
  39. Trapping and apoptosis of novel subsets of memory T lymphocytes expressing CCR6 in the spleen of HIV-infected patients.
    Authors: Lecureuil C, Combadiere B, Mazoyer E, Bonduelle O, Samri A, Autran B, Debre P, Combadiere C
    Blood, 2007;109(9):3649-57.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  40. Chemokine receptors in head and neck cancer: association with metastatic spread and regulation during chemotherapy.
    Authors: Muller A, Sonkoly E, Eulert C, Gerber PA, Kubitza R, Schirlau K, Franken-Kunkel P, Poremba C, Snyderman C, Klotz LO, Ruzicka T, Bier H, Zlotnik A, Whiteside TL, Homey B, Hoffmann TK
    Int. J. Cancer, 2006;118(9):2147-57.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  41. Epithelial inflammation is associated with CCL28 production and the recruitment of regulatory T cells expressing CCR10.
    Authors: Eksteen B, Miles A, Curbishley SM, Tselepis C, Grant AJ, Walker LS, Adams DH
    J. Immunol., 2006;177(1):593-603.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  42. The apoptotic-cell receptor CR3, but not alphavbeta5, is a regulator of human dendritic-cell immunostimulatory function.
    Authors: Skoberne M, Somersan S, Almodovar W, Truong T, Petrova K, Henson PM, Bhardwaj N
    Blood, 2006;108(3):947-55.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  43. Recapitulation of B cell differentiation in the central nervous system of patients with multiple sclerosis.
    Authors: Casazza S, Zappia E, Pistorio A, Gambini C
    Proc. Natl. Acad. Sci. U.S.A., 2004;101(30):11064-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  44. In vitro-expanded human CD4(+)CD25(+) T-regulatory cells can markedly inhibit allogeneic dendritic cell-stimulated MLR cultures.
    Authors: Godfrey WR, Ge YG, Spoden DJ, Levine BL, June CH, Blazar BR, Porter SB
    Blood, 2004;104(2):453-61.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  45. Human cerebrospinal fluid central memory CD4+ T cells: evidence for trafficking through choroid plexus and meninges via P-selectin.
    Authors: Kivisakk P, Mahad DJ, Callahan MK, Trebst C, Tucky B, Wei T, Wu L, Baekkevold ES, Lassmann H, Staugaitis SM, Campbell JJ, Ransohoff RM
    Proc. Natl. Acad. Sci. U.S.A., 2003;100(14):8389-94.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  46. Chemokine receptor expression on human eosinophils from peripheral blood and bronchoalveolar lavage fluid after segmental antigen challenge.
    Authors: Liu LY, Jarjour NN, Busse WW, Kelly EA
    J. Allergy Clin. Immunol., 2003;112(3):556-62.
    Species: Human
    Sample Type: BALF
    Application: Flow
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Isotype Controls
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Mouse IgG2A PE-conjugated Antibody

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Flow Cytometry Mouse Lyse Buffer (10X)

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Flow Cytometry Human Lyse Buffer (10X)

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We have 1 review tested in 1 application: Flow Cytometry.

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Flow Cytometry Human CCR7 PE‑conjugated Antibody FAB197P
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Flow Cytometry Human CCR7 PE‑conjugated Antibody FAB197P
Flow Cytometry: Human CCR7 PE‑conjugated Antibody [FAB197P]

Summary

ApplicationFlow Cytometry
Sample TestedPeripheral blood cells
SpeciesHuman

Other Experimental Details

Other Experimental DetailsGated on lymphocyte FSC-A and SSC-A populations. Singlets were further gated on by FSC-A/FSC-H doublet exclusion. Lineage Cocktail 1 (BD Biosciences) comprises CD3, CD14, CD16, CD19, CD20, and CD56 antibodies.
Specificity: Specific
Sensitivity: Sensitive
Buffer: 2% Human Serum, 0.5 mM EDTA in PBS
Dilution: 1/100

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