Human CCR7 APC-conjugated Antibody

Clone 150503 was used by HLDA to establish CD designation.
(12 citations)
(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human CCR7.
  • Source
    Monoclonal Mouse IgG2A Clone # 150503
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Human CCR7 transfectants
    Met1-Pro378
    Accession # AAA58615
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CCR7 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD4 PE‑conjugated Monoclonal Antibody (Catalog # FAB3791P) and either (A) Mouse Anti-Human CCR7 APC‑conjugated Monoclonal Antibody (Catalog # FAB197A) or (B) Mouse IgG2A Allophycocyanin Isotype Control (Catalog # IC003A). View our protocol for Staining Membrane-associated Proteins.
Detection of CCR7 in HEK293 Human Cell Line Transfected with Human CCR7 and eGFP by Flow Cytometry. The specificity of Mouse Anti-Human CCR7 Clone 150503 was demonstrated by its ability to react with (A) HEK293 human embryonic kidney cell line transfected with human CCR7 and not react with (B) irrelevant HEK293 transfectants. Antibody binding was monitored using Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CCR7

CCR7 is a 7 transmembrane G protein coupled chemokine receptor. CCR7 is expressed on T cells and mature dendritic cells and transduces chemotactic signals in response to CCL19 and CCL21. Human CCR7 shares 87% amino acid sequence identity with mouse CCR7.

  • Entrez Gene IDs:
    1236 (Human); 12775 (Mouse); 287673 (Rat)
  • Alternate Names:
    BLR2C-C chemokine receptor type 7; C-C CKR-7; CC-CKR-7; CCR7; CCR-7; CD197 antigen; CD197; CDw197CC chemokine receptor 7; chemokine (C-C motif) receptor 7; CMKBR7chemokine (C-C) receptor 7; EBI1EBV-induced G protein-coupled receptor 1; EBV-induced G-protein coupled receptor 1; Epstein-Barr virus induced gene 1; Epstein-Barr virus induced G-protein coupled receptor; Epstein-Barr virus-induced G-protein coupled receptor 1; EVI1; lymphocyte-specific G protein-coupled peptide receptor; MIP-3 beta receptor
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. A high migratory capacity of donor T-cells in response to the lymph node homing receptor CCR7 increases the incidence and severity of GvHD
    Authors: I Portero-Sa, V G¢mez-Garc, C Cuesta-Mat, C Fern ndez-, L Vega-Piris, M Royg, B Colom-Fern, A Marcos-Jim, B Somovilla-, A Ram¡rez-Me, V L¢pez-Huet, A de Rosendo, A Kreutzman, C Mu¤oz-Call
    Bone Marrow Transplant, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Enhancement of cytokine-driven NK cell IFN-? production after vaccination of HCMV infected Africans
    Authors: A Darboe, E Danso, E Clarke, A Umesi, E Touray, R Wegmuller, SE Moore, EM Riley, MR Goodier
    Eur. J. Immunol., 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Codelivery of Envelope Protein in Alum with MVA Vaccine Induces CXCR3-Biased CXCR5+ and CXCR5- CD4 T Cell Responses in Rhesus Macaques.
    Authors: Iyer S, Gangadhara S, Victor B, Gomez R, Basu R, Hong J, Labranche C, Montefiori D, Villinger F, Moss B, Amara R
    J Immunol, 2015;195(3):994-1005.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Type: Whole Cells
    Application: Flow
  4. Intramuscular delivery of heterodimeric IL-15 DNA in macaques produces systemic levels of bioactive cytokine inducing proliferation of NK and T cells.
    Authors: Bergamaschi C, Kulkarni V, Rosati M, Alicea C, Jalah R, Chen S, Bear J, Sardesai N, Valentin A, Felber B, Pavlakis G
    Gene Ther, 2015;22(1):76-86.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Type: Whole Cells
    Application: Flow
  5. Lymphocyte maintenance during healthy aging requires no substantial alterations in cellular turnover.
    Authors: Westera L, van Hoeven V, Drylewicz J, Spierenburg G, van Velzen J, de Boer R, Tesselaar K, Borghans J
    Aging Cell, 2015;14(2):219-27.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  6. IFNgamma/IL-10 co-producing cells dominate the CD4 response to malaria in highly exposed children.
    Authors: Jagannathan P, Eccles-James I, Bowen K, Nankya F, Auma A, Wamala S, Ebusu C, Muhindo M, Arinaitwe E, Briggs J, Greenhouse B, Tappero J, Kamya M, Dorsey G, Feeney M
    PLoS Pathog, 2014;10(1):e1003864.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  7. Chemokines as novel and versatile reagents for flow cytometry and cell sorting.
    Authors: Le Brocq M, Fraser A, Cotton G, Woznica K, McCulloch C, Hewit K, McKimmie C, Nibbs R, Campbell J, Graham G
    J Immunol, 2014;192(12):6120-30.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  8. Heterologous vaccination against human tuberculosis modulates antigen-specific CD4+ T-cell function.
    Authors: Dintwe O, Day C, Smit E, Nemes E, Gray C, Tameris M, McShane H, Mahomed H, Hanekom W, Scriba T
    Eur J Immunol, 2013;43(9):2409-20.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  9. Shaping of iNKT cell repertoire after unrelated cord blood transplantation.
    Authors: Beziat V, Nguyen S, Exley M, Achour A, Simon T, Chevallier P, Sirvent A, Vigouroux S, Debre P, Rio B, Vieillard V, Lubrano-Berthelier C, Alatrakchi N
    Clin. Immunol., 2010;135(3):364-73.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  10. Human dendritic cells acquire a semimature phenotype and lymph node homing potential through interaction with CD4+CD25+ regulatory T cells.
    Authors: Bayry J, Triebel F, Kaveri SV, Tough DF
    J. Immunol., 2007;178(7):4184-93.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  11. Trapping and apoptosis of novel subsets of memory T lymphocytes expressing CCR6 in the spleen of HIV-infected patients.
    Authors: Lecureuil C, Combadiere B, Mazoyer E, Bonduelle O, Samri A, Autran B, Debre P, Combadiere C
    Blood, 2007;109(9):3649-57.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  12. IL-7Ralpha expression on CD4+ T lymphocytes decreases with HIV disease progression and inversely correlates with immune activation.
    Authors: Koesters SA, Alimonti JB, Wachihi C, Matu L, Anzala O, Kimani J, Embree JE, Plummer FA, Fowke KR
    Eur. J. Immunol., 2006;36(2):336-44.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Expand to show all 12 Citations
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A APC-conjugated Antibody

Ctrl IC003A 2  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Flow Cytometry.

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 Flow Human Anonymous 12/11/2017
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Summary

ApplicationFlow Cytometry
Sample TestedBlood mononuclear cells (PBMCs)
SpeciesHuman

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