Human CCR7 Fluorescein-conjugated Antibody

Clone 150503 was used by HLDA to establish CD designation.
(46 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human CCR7.
  • Source
    Monoclonal Mouse IgG2A Clone # 150503
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Human CCR7 transfectants
    Met1-Pro378
    Accession # AAA58615
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Fluorescein
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CCR7 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD4 APC‑conjugated Monoclonal Antibody (Catalog # FAB3791A) and either (A) Mouse Anti-Human CCR7 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB197F) or (B) Mouse IgG2A Fluorescein Isotype Control (Catalog # IC003F). View our protocol for Staining Membrane-associated Proteins.
Detection of CCR7 in HEK293 Human Cell Line Transfected with Human CCR7 and eGFP by Flow Cytometry. The specificity of Mouse Anti-Human CCR7 Clone 150503 was demonstrated by its ability to react with (A) HEK293 human embryonic kidney cell line transfected with human CCR7 and not react with (B) irrelevant HEK293 transfectants. Antibody binding was monitored using Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CCR7

CCR7 is a 7 transmembrane G protein coupled chemokine receptor. CCR7 is expressed on T cells and mature dendritic cells and transduces chemotactic signals in response to CCL19 and CCL21. Human CCR7 shares 87% amino acid sequence identity with mouse CCR7.

  • Entrez Gene IDs:
    1236 (Human); 12775 (Mouse); 287673 (Rat)
  • Alternate Names:
    BLR2C-C chemokine receptor type 7; C-C CKR-7; CC-CKR-7; CCR7; CCR-7; CD197 antigen; CD197; CDw197CC chemokine receptor 7; chemokine (C-C motif) receptor 7; CMKBR7chemokine (C-C) receptor 7; EBI1EBV-induced G protein-coupled receptor 1; EBV-induced G-protein coupled receptor 1; Epstein-Barr virus induced gene 1; Epstein-Barr virus induced G-protein coupled receptor; Epstein-Barr virus-induced G-protein coupled receptor 1; EVI1; lymphocyte-specific G protein-coupled peptide receptor; MIP-3 beta receptor
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

46 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. pERK-dependent defective TCR-mediated activation of CD4(+) T cells in end-stage renal disease patients
    Authors: L Huang, NHR Litjens, NM Kannegiete, M Klepper, CC Baan, MGH Betjes
    Immun Ageing, 2017;14(0):14.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Regeneration of long-distance peripheral nerve defects after delayed reconstruction in healthy and diabetic rats is supported by immunomodulatory chitosan nerve guides
    Authors: L Stenberg, M Stö beta el, G Ronchi, S Geuna, Y Yin, S Mommert, L Mårtensson, J Metzen, C Grothe, LB Dahlin, K Haastert-T
    BMC Neurosci, 2017;18(1):53.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. NKG2D ligand targeted Bispecific T Cell Engagers lead to robust antitumor activity against diverse human tumors
    Authors: C Godbersen, TA Coupet, AM Huehls, T Zhang, MB Battles, JL Fisher, MS Ernstoff, CL Sentman
    Mol. Cancer Ther., 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. Natural killer cell counts are associated with molecular relapse-free survival after imatinib discontinuation in chronic myeloid leukemia: the IMMUNOSTIM study
    Authors: D Rea, G Henry, Z Khaznadar, G Etienne, F Guilhot, F Nicolini, J Guilhot, P Rousselot, F Huguet, L Legros, M Gardembas, V Dubruille, A Guerci-Bre, A Charbonnie, F Maloisel, JC Ianotto, B Villemagne, FX Mahon, H Moins-Teis, N Dulphy, A Toubert
    Haematologica, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. Envelope residue 375 substitutions in simian-human immunodeficiency viruses enhance CD4 binding and replication in rhesus macaques
    Authors: Hui Li
    Proc Natl Acad Sci USA, 2016;113(24):E3413-22.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  6. Effective Cytotoxic T Lymphocyte Targeting of Persistent HIV-1 during Antiretroviral Therapy Requires Priming of Naive CD8+ T Cells
    Authors: Kellie N Smith
    MBio, 2016;7(3):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  7. Glycolysis determines dichotomous regulation of T cell subsets in hypoxia
    Authors: Yang Xu
    J Clin Invest, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  8. Cytomegalovirus-Induced Expression of CD244 after Liver Transplantation Is Associated with CD8+ T Cell Hyporesponsiveness to Alloantigen.
    Authors: de Mare-Bredemeijer E, Shi X, Mancham S, van Gent R, van der Heide-Mulder M, de Boer R, Heemskerk M, de Jonge J, van der Laan L, Metselaar H, Kwekkeboom J
    J Immunol, 2015;195(4):1838-48.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  9. Lymphocyte gene expression signatures from patients and mouse models of hereditary hemochromatosis reveal a function of HFE as a negative regulator of CD8+ T-lymphocyte activation and differentiation in vivo.
    Authors: Costa M, Cruz E, Oliveira S, Benes V, Ivacevic T, Silva M, Vieira I, Dias F, Fonseca S, Goncalves M, Lima M, Leitao C, Muckenthaler M, Pinto J, Porto G
    PLoS ONE, 2015;10(4):e0124246.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  10. CD40L induces functional tunneling nanotube networks exclusively in dendritic cells programmed by mediators of type 1 immunity.
    Authors: Zaccard C, Watkins S, Kalinski P, Fecek R, Yates A, Salter R, Ayyavoo V, Rinaldo C, Mailliard R
    J Immunol, 2015;194(3):1047-56.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  11. STAT3 is a critical cell-intrinsic regulator of human unconventional T cell numbers and function.
    Authors: Wilson R, Ives M, Rao G, Lau A, Payne K, Kobayashi M, Arkwright P, Peake J, Wong M, Adelstein S, Smart J, French M, Fulcher D, Picard C, Bustamante J, Boisson-Dupuis S, Gray P, Stepensky P, Warnatz K, Freeman A, Rossjohn J, McCluskey J, Holland S, Casanova J, Uzel G, Ma C, Tangye S, Deenick E
    J Exp Med, 2015;212(6):855-64.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  12. Evaluating Human T-Cell Therapy of Cytomegalovirus Organ Disease in HLA-Transgenic Mice.
    Authors: Thomas S, Klobuch S, Podlech J, Plachter B, Hoffmann P, Renzaho A, Theobald M, Reddehase M, Herr W, Lemmermann N
    PLoS Pathog, 2015;11(7):e1005049.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  13. Human Non-neutralizing HIV-1 Envelope Monoclonal Antibodies Limit the Number of Founder Viruses during SHIV Mucosal Infection in Rhesus Macaques.
    Authors: Santra S, Tomaras G, Warrier R, Nicely N, Liao H, Pollara J, Liu P, Alam S, Zhang R, Cocklin S, Shen X, Duffy R, Xia S, Schutte R, Pemble Iv C, Dennison S, Li H, Chao A, Vidnovic K, Evans A, Klein K, Kumar A, Robinson J, Landucci G, Forthal D, Montefiori D, Kaewkungwal J, Nitayaphan S, Pitisuttithum P, Rerks-Ngarm S, Robb M, Michael N, Kim J, Soderberg K, Giorgi E, Blair L, Korber B, Moog C, Shattock R, Letvin N, Schmitz J, Moody M, Gao F, Ferrari G, Shaw G, Haynes B
    PLoS Pathog, 2015;11(8):e1005042.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  14. Tetramer enrichment reveals the presence of phenotypically diverse hepatitis C virus-specific CD8+ T cells in chronic infection.
    Authors: Nitschke K, Flecken T, Schmidt J, Gostick E, Marget M, Neumann-Haefelin C, Blum H, Price D, Thimme R
    J Virol, 2015;89(1):25-34.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  15. Peripheral blood CD56(bright) NK cells respond to stem cell factor and adhere to its membrane-bound form after upregulation of c-kit.
    Authors: Pradier A, Tabone-Eglinger S, Huber V, Bosshard C, Rigal E, Wehrle-Haller B, Roosnek E
    Eur J Immunol, 2014;44(2):511-20.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  16. Severe acute respiratory syndrome-coronavirus infection in aged nonhuman primates is associated with modulated pulmonary and systemic immune responses.
    Authors: Clay C, Donart N, Fomukong N, Knight J, Overheim K, Tipper J, Van Westrienen J, Hahn F, Harrod K
    Immun Ageing, 2014;11(1):4.
    Species: Primate - Chlorocebus pygerythrus (Vervet Monkey)
    Sample Type: Tissue Homogenates
    Application: Flow
  17. MART-1 peptide vaccination plus IMP321 (LAG-3Ig fusion protein) in patients receiving autologous PBMCs after lymphodepletion: results of a Phase I trial.
    Authors: Romano E, Michielin O, Voelter V, Laurent J, Bichat H, Stravodimou A, Romero P, Speiser D, Triebel F, Leyvraz S, Harari A
    J Transl Med, 2014;12(0):97.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  18. Exhaustion of bacteria-specific CD4 T cells and microbial translocation in common variable immunodeficiency disorders.
    Authors: Perreau M, Vigano S, Bellanger F, Pellaton C, Buss G, Comte D, Roger T, Lacabaratz C, Bart P, Levy Y, Pantaleo G
    J Exp Med, 2014;211(10):2033-45.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  19. Tracking in vivo dynamics of NK cells transferred in patients undergoing stem cell transplantation.
    Authors: Killig M, Friedrichs B, Meisig J, Gentilini C, Bluthgen N, Loddenkemper C, Labopin M, Basara N, Pfrepper C, Niederwieser D, Uharek L, Romagnani C
    Eur J Immunol, 2014;44(9):2822-34.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  20. Cytomegalovirus drives Vdelta2neg gammadelta T cell inflation in many healthy virus carriers with increasing age.
    Authors: Alejenef A, Pachnio A, Halawi M, Christmas S, Moss P, Khan N
    Clin Exp Immunol, 2014;176(3):418-28.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  21. Both HLA-B*57 and plasma HIV RNA levels contribute to the HIV-specific CD8+ T cell response in HIV controllers.
    Authors: Lecuroux C, Saez-Cirion A, Girault I, Versmisse P, Boufassa F, Avettand-Fenoel V, Rouzioux C, Meyer L, Pancino G, Lambotte O, Sinet M, Venet A
    J Virol, 2014;88(1):176-87.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  22. IL-12 directs further maturation of ex vivo differentiated NK cells with improved therapeutic potential.
    Authors: Lehmann, Dorit, Spanholtz, Jan, Sturtzel, Caterina, Tordoir, Marleen, Schlechta, Bernhard, Groenewegen, Dirk, Hofer, Erhard
    PLoS ONE, 2014;9(1):e87131.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  23. IL-27 in human secondary lymphoid organs attracts myeloid dendritic cells and impairs HLA class I-restricted antigen presentation.
    Authors: Morandi, Fabio, Di Carlo, Emma, Ferrone, Soldano, Petretto, Andrea, Pistoia, Vito, Airoldi, Irma
    J Immunol, 2014;192(6):2634-42.
  24. Concurrent interaction of DCs with CD4(+) and CD8(+) T cells improves secondary CTL expansion: It takes three to tango.
    Authors: Hoyer S, Prommersberger S, Pfeiffer I, Schuler-Thurner B, Schuler G, Dorrie J, Schaft N
    Eur J Immunol, 2014;44(12):3543-59.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  25. CCR5 is a receptor for Staphylococcus aureus leukotoxin ED.
    Authors: Alonzo, Francis, Kozhaya, Lina, Rawlings, Stephen, Reyes-Robles, Tamara, DuMont, Ashley L, Myszka, David G, Landau, Nathanie, Unutmaz, Derya, Torres, Victor J
    Nature, 2013;493(7430):51-5.
    Species: Human
    Sample Type: Whole Cells
  26. IL-18-based combinatorial adjuvants promote the intranodal production of CCL19 by NK cells and dendritic cells of cancer patients.
    Authors: Wong J, Muthuswamy R, Bartlett D, Kalinski P
    Oncoimmunology, 2013;2(9):e26245.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  27. Bone marrow T cells from the femur are similar to iliac crest derived cells in old age and represent a useful tool for studying the aged immune system.
    Authors: Pritz T, Landgraf-Rauf K, Herndler-Brandstetter D, Rauf R, Lair J, Gassner R, Weinberger B, Krismer M, Grubeck-Loebenstein B
    Immun Ageing, 2013;10(1):17.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  28. Biomarkers on melanoma patient T cells associated with ipilimumab treatment.
    Authors: Wang W, Yu D, Sarnaik A, Yu B, Hall M, Morelli D, Zhang Y, Zhao X, Weber J
    J Transl Med, 2012;10(0):146.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  29. Targeting of macrophage galactose-type C-type lectin (MGL) induces DC signaling and activation.
    Authors: Napoletano C, Zizzari IG, Rughetti A, Rahimi H, Irimura T, Clausen H, Wandall HH, Belleudi F, Bellati F, Pierelli L, Frati L, Nuti M
    Eur. J. Immunol., 2012;42(4):936-45.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  30. The CD46-Jagged1 interaction is critical for human TH1 immunity.
    Authors: Le Friec G, Sheppard D, Whiteman P, Karsten C, Shamoun S, Laing A, Bugeon L, Dallman M, Melchionna T, Chillakuri C, Smith R, Drouet C, Couzi L, Fremeaux-Bacchi V, Kohl J, Waddington S, McDonnell J, Baker A, Handford P, Lea S, Kemper C
    Nat Immunol, 2012;13(12):1213-21.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  31. CCR2+CCR5+ T Cells Produce Matrix Metalloproteinase-9 and Osteopontin in the Pathogenesis of Multiple Sclerosis.
    Authors: Sato W, Tomita A, Ichikawa D, Lin Y, Kishida H, Miyake S, Ogawa M, Okamoto T, Murata M, Kuroiwa Y, Aranami T, Yamamura T
    J Immunol, 2012;189(10):5057-65.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  32. Identification of human NK17/NK1 cells.
    Authors: Pandya AD, Al-Jaderi Z, Hoglund RA, Holmoy T, Harbo HF, Norgauer J, Maghazachi AA
    PLoS ONE, 2011;6(10):e26780.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  33. Primary B-cell deficiencies reveal a link between human IL-17-producing CD4 T-cell homeostasis and B-cell differentiation.
    Authors: Barbosa RR, Silva SP, Silva SL, Melo AC, Pedro E, Barbosa MP, Pereira-Santos MC, Victorino RM, Sousa AE
    PLoS ONE, 2011;6(8):e22848.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  34. Hypoxia modulates the gene expression profile of immunoregulatory receptors in human mature dendritic cells: identification of TREM-1 as a novel hypoxic marker in vitro and in vivo.
    Authors: Bosco MC, Pierobon D, Blengio F, Raggi F, Vanni C, Gattorno M, Eva A, Novelli F, Cappello P, Giovarelli M, Varesio L
    Blood, 2011;117(9):2625-39.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  35. Phenotypic and functional markers for 1alpha,25-dihydroxyvitamin D(3)-modified regulatory dendritic cells.
    Authors: Pedersen AW, Holmstrom K, Jensen SS, Fuchs D, Rasmussen S, Kvistborg P, Claesson MH, Zocca MB
    Clin. Exp. Immunol., 2010;157(1):48-59.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  36. CCR2 identifies a stable population of human effector memory CD4+ T cells equipped for rapid recall response.
    Authors: Zhang HH, Song K, Rabin RL, Hill BJ, Perfetto SP, Roederer M, Douek DC, Siegel RM, Farber JM
    J. Immunol., 2010;185(11):6646-63.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  37. Loss of CD4+ T cell IL-6R expression during inflammation underlines a role for IL-6 trans signaling in the local maintenance of Th17 cells.
    Authors: Jones GW, McLoughlin RM, Hammond VJ, Parker CR, Williams JD, Malhotra R, Scheller J, Williams AS, Rose-John S, Topley N, Jones SA
    J. Immunol., 2010;184(4):2130-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  38. Expression of soluble vascular endothelial growth factor receptor-1 in human monocyte-derived mature dendritic cells contributes to their antiangiogenic property.
    Authors: Kishuku M, Nishioka Y, Abe S, Kishi J, Ogino H, Aono Y, Azuma M, Kinoshita K, Batmunkh R, Rentsenhand B, Makino H, Ranjan P, Minakuchi K, Sone S
    J. Immunol., 2009;183(12):8176-85.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  39. CCL21 overexpressed on lymphatic vessels drives thymic hyperplasia in myasthenia.
    Authors: Berrih-Aknin S, Ruhlmann N, Bismuth J, Cizeron-Clairac G, Zelman E, Shachar I, Dartevelle P, de Rosbo NK, Le Panse R
    Ann. Neurol., 2009;66(4):521-31.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  40. Changes in chemokines and chemokine receptor expression on tonsillar B cells upon Epstein-Barr virus infection.
    Authors: Ehlin-Henriksson B, Liang W, Cagigi A, Mowafi F, Klein G, Nilsson A
    Immunology, 2009;127(4):549-57.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  41. An unusual CD56(bright) CD16(low) NK cell subset dominates the early posttransplant period following HLA-matched hematopoietic stem cell transplantation.
    Authors: Dulphy N, Haas P, Busson M, Belhadj S, Peffault de Latour R, Robin M, Carmagnat M, Loiseau P, Tamouza R, Scieux C, Rabian C, Di Santo JP, Charron D, Janin A, Socie G, Toubert A
    J. Immunol., 2008;181(3):2227-37.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  42. Potential role for IL-7 in Fas-mediated T cell apoptosis during HIV infection.
    Authors: Fluur C, De Milito A, Fry TJ, Vivar N, Eidsmo L, Atlas A, Federici C, Matarrese P, Logozzi M, Rajnavolgyi E, Mackall CL, Fais S, Chiodi F, Rethi B
    J. Immunol., 2007;178(8):5340-50.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  43. Alpha1beta1 integrin is crucial for accumulation of epidermal T cells and the development of psoriasis.
    Authors: Conrad C, Boyman O, Tonel G, Tun-Kyi A, Laggner U, de Fougerolles A, Kotelianski V, Gardner H, Nestle FO
    Nat. Med., 2007;13(7):836-42.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  44. Intravenous immunoglobulins suppress T-cell priming by modulating the bidirectional interaction between dendritic cells and natural killer cells.
    Authors: Tha-In T, Metselaar HJ, Tilanus HW, Groothuismink ZM, Kuipers EJ, De Man RA, Kwekkeboom J
    Blood, 2007;110(9):3253-62.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  45. Aberration of CCR7 CD8 memory T cells from patients with systemic lupus erythematosus: an inducer of T helper type 2 bias of CD4 T cells.
    Authors: Sen Y, Chunsong H, Baojun H, Linjie Z, Qun L, San J, Qiuping Z, Junyan L, Zhang X, Jinquan T
    Immunology, 2004;112(2):274-89.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  46. TCR affinity associated with functional differences between dominant and subdominant SIV epitope-specific CD8+ T cells in Mamu-A*01+ rhesus monkeys.
    Authors: Osuna C, Gonzalez A, Chang H, Hung A, Ehlinger E, Anasti K, Alam S, Letvin N
    PLoS Pathog, 0;10(4):e1004069.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Expand to show all 46 Citations
Isotype Controls
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Mouse IgG2A Fluorescein-conjugated Antibody

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Flow Cytometry Mouse Lyse Buffer (10X)

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Flow Cytometry Human Lyse Buffer (10X)

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