Human CD117/c-kit Quantikine ELISA Kit

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  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL)
  • Sensitivity
    0.339 ng/mL
  • Assay Range
    1.6 - 50 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant human SCF sR
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
Product Summary
The Quantikine Human SCF sR Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human SCF sR in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant human SCF sR and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human SCF sR showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human SCF sR.

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Standard Deviation0.2980.3590.5620.5550.6881.76

Cell Culture Supernates
Intra-Assay Precision Inter-Assay Precision
Standard Deviation0.2280.390.7070.4810.5091.21


The recovery of SCF sR spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 103 97-110
EDTA Plasma (n=4) 91 84-106
Heparin Plasma (n=4) 92 87-102
Serum (n=4) 93 86-115
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of SCF sR were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human CD117/c-kit Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CD117/c-kit
CD117, also known as c-kit and SCF R, is the cellular receptor for Stem Cell Factor (SCF), also named c-kit ligand, mast cell growth factor, and steel factor. This receptor-ligand system plays essential roles in germ cell development, melanogenesis, hematopoiesis, and oncogenesis. CD117 is expressed on lymphoid, myeloid, erythroid, and megakaryocyte progenitors, NK cells, germ cells, melanocytes, glial cells, vascular smooth muscle cells, placenta, and epithelial cells. Altered levels and mutations in CD117 are associated with several types of cancer including lung, breast, gastrointestinal stromal, and germ cell tumors.
    • Long Name
      Stem Cell Factor Receptor
    • Entrez Gene IDs
      3815 (Human); 16590 (Mouse); 64030 (Rat);
    • Alternate Names
      CD117 antigen; CD117; ckit; c-kit; EC 2.7.10; EC; KIT; PBT; piebald trait; Proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; SCF R; SCFRmast/stem cell growth factor receptor; soluble KIT variant 1; Tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 100 µL Assay Diluent
    4.   Add 100 µL of Assay Diluent to each well.

    5. 100 µL Standard, Control, or Sample
    6.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    10.   Aspirate and wash 4 times.

    11. 200 µL Substrate Solution
    12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    13. 50 µL Stop Solution
    14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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    Sample Type
    1. Analysis of plasma cytokines and angiogenic factors in patients with pretreated urothelial cancer receiving Pazopanib: the role of circulating interleukin-8 to enhance the prognostic accuracy.
      Authors: Necchi A, Pennati M, Zaffaroni N, Landoni E, Giannatempo P, Raggi D, Schwartz L, Morosi C, Crippa F, Fare E, Nicolai N, Lanocita R, Sava T, Sacco C, Messina C, Ortega C, De Braud F, Salvioni R, Daidone M, Gianni A, Mariani L
      Br J Cancer, 2014;110(1):26-33.
      Species: Human
      Sample Type: Plasma
    2. Plasma biomarkers as predictors of outcome in patients with advanced hepatocellular carcinoma.
      Authors: Llovet JM, Pena CE, Lathia CD, Shan M, Meinhardt G, Bruix J, Pallota MG, Zarba JJ, Boyer M, Riordan S, Strickland A, Tebbutt N, Thomson B, Borbath I, De Greve J, Van Laethem J-, Van Steenbergen W, Van Vlierberghe H, Barrios C, Cosme de Oliveira A, Kotzev I, Takov D, Tchernev K, Burak K, Ma M, Metrakos P, Olweny C, Sherman M, Gamargo Garate C, Martinez-Castillo J, Beaugrand M, Bennouna J, Blanc J-, Bronowicki J-, Degos F, Dominguez S, Grange J-, Hillon P, Raoul J-, Seitz J-, Blum H, Buggisch P, Caspary W, Dollinger M, Gerken G, Goke B, Gregor M, Greten T, Haussinger D, Hilgard P, Scherübl J, Scheulen M, Schmid R, Spengler U, Wiest R, Zeuzem S, Arvanitakis C, Germanidis G, Katsos I, Figer A, Stemmer S, Amadori D, Bolondi L, Cognetti F, Craxi A, Farinati F, Gridelli C, Martoni A, Mazzaferro V, Porta C, Ricci S, Sangiovanni A, Santoro A, Trevisani F, Cisnero Garza LE, Gane E, O'Donnell A, Leon J, Lozano A, Jassem J, Rydzewska G, Szawlowski A, Tomczak P, Badulescu F, Miron L, Kubyshkin V, Bruix J, Forner A, Bustamante Schneider J, Diago M, Montero Alvarez JL, Pascual S, Ruíz del Arbol L, Sangro B, Solá R, Tabernero J, Muellhaupt B, Roth A, Jeffry Evans TR, Falk S, Meyer T, Reeves H, Ross P, Befeler A, Boyer T, Britten C, Byrne T, Garcia-Tsao G, Gold P, Goldenberg A, Heuman D, Kennedy P, Koch A, Llovet JM, Marrero J, Schilsky M, Schwartz J, Schwartz M
      Clin. Cancer Res., 2012;18(8):2290-300.
      Species: Human
      Sample Type: Plasma
    3. Efficacy, safety, and potential biomarkers of sunitinib monotherapy in advanced hepatocellular carcinoma: a phase II study.
      Authors: Zhu AX, Sahani DV, Duda DG, di Tomaso E, Ancukiewicz M, Catalano OA, Sindhwani V, Blaszkowsky LS, Yoon SS, Lahdenranta J, Bhargava P, Meyerhardt J, Clark JW, Kwak EL, Hezel AF, Miksad R, Abrams TA, Enzinger PC, Fuchs CS, Ryan DP, Jain RK
      J. Clin. Oncol., 2009;27(18):3027-35.
      Species: Human
      Sample Type: Plasma
    4. Endothelial progenitor cell levels in obese men with the metabolic syndrome and the effect of simvastatin monotherapy vs. simvastatin/ezetimibe combination therapy.
      Authors: Westerweel PE, Visseren FL, Hajer GR, Olijhoek JK, Hoefer IE, de Bree P, Rafii S, Doevendans PA, Verhaar MC
      Eur. Heart J., 2008;29(22):2808-17.
      Species: Human
      Sample Type: Serum
    5. Phase II trial of sunitinib in patients with metastatic colorectal cancer after failure of standard therapy.
      Authors: Saltz LB, Rosen LS, Marshall JL, Belt RJ, Hurwitz HI, Eckhardt SG, Bergsland EK, Haller DG, Lockhart AC, Rocha Lima CM, Huang X, DePrimo SE, Chow-Maneval E, Chao RC, Lenz HJ
      J. Clin. Oncol., 2007;25(30):4793-9.
      Species: Human
      Sample Type: Plasma
    ELISA Controls
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    Quantikine Immunoassay Control Group 8

    Ctrl QC23
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    Quantikine Wash Buffer 1

    ELISA WA126 5
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