Detection of CD27Ligand/TNFSF7 in THP-1 Human Cell Line by Flow Cytometry.
THP‑1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human CD27 Ligand/TNFSF7 Monoclonal Antibody (Catalog # MAB27381, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram) followed by Goat anti-mouse PE-conjugated secondary antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD27 Ligand/TNFSF7
Human CD27 Ligand (also CD70 and TNFSF7) is a 30 kDa type 2 transmembrane glycoprotein that is a member of the TNF superfamily. It contains a 20 amino acid (aa) cytoplasmic region and a 155 aa extracellular domain that shows multiple beta -strands. It is an inducible trimer that occurs on B cells, T cells, and NK cells. It regulates T cell dependant B cell differentiation into plasma cells and likely to promotes clonal expansion of T cells. Human CD27 Ligand extracellular region is 64% aa identical to mouse and rat CD27 Ligand extracellular regions.
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