Detects human CD300b/LMIR5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross-reactivity with recombinant human (rh) LMIR3 is observed and less than 5% cross-reactivity with rhLMIR1, -2, -6 and recombinant mouse LMIR4 and -5 is observed.
Polyclonal Goat IgG
Mouse myeloma cell line NS0-derived recombinant human CD300b/LMIR5 Ile55-His187 Accession # NP_777552
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Recombinant Human CD300b/LMIR5
2.5 µg/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of CD300b/LMIR5 in Human Monocytes by Flow Cytometry. Human whole blood monocytes were stained with Goat Anti-Human CD300b/LMIR5 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF2879, filled histogram) or control antibody (Catalog # AB‑108‑C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
CD300b, also known asLMIR5, CLM7 (CMRF-35-like Molecule 7) and TREM5 (Triggering Receptor Expressed on Myeloid cells 5) belongs to a multigene family whose members are closely mapped to a region of human chromosome 17 and mouse chromosome 11. These genes encode type I membrane proteins with a single extracellular Ig-like domain and the proteins are expressed widely in leukocytes. LMIR/CD300 family proteins are immunoregulatory signaling molecules that either have cytoplasmic ITIM motifs or can interact with ITAM motif-bearing molecules through a characteristic transmembrane domain containing positively charged amino acid residues. CD300b/LMIR5 is an ITAM adaptor-associated receptor with a short cytoplasmic region. Human and mouse CD300b/LMIR5 share approximately 45% amino acid sequence identity.
Leukocyte Mono Ig-like Receptor 5
Entrez Gene IDs:
124599 (Human); 217304 (Mouse)
CD300 antigen like family member B; CD300 antigen-like family member B; CD300 molecule-like family member b; CD300b antigen; CD300b; CD300LB; CLM7; CLM-7; CLM7IREM3CD300 molecule like family member b; CMRF35A2; CMRF35-A2; CMRF35-like molecule 7; EC 184.108.40.206; EC 220.127.116.11; Gm253; Immune receptor expressed on myeloid cells 3; IREM-3; Leukocyte mono-Ig-like receptor 5; LMIR5; TREM-5; TREM5CD300b; Triggering receptor expressed on myeloid cells 5
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.