Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, CyTOF-ready

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all CD300b/LMIR5 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CD300b/LMIR5
Ile55-His187
Accession # NP_777552

Specificity

Detects human CD300b/LMIR5 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human LMIR2 and recombinant mouse LMIR5 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human CD300b/LMIR5 Antibody

Detection of CD300b/LMIR5 antibody in Human Monocytes antibody by Flow Cytometry.

Detection of CD300b/LMIR5 in Human Monocytes by Flow Cytometry.

Human whole blood monocytes were stained with Goat Anti-Human CD300b/LMIR5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2879, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Applications for Human CD300b/LMIR5 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Human whole blood monocytes

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD300b/LMIR5

CD300b, also known as LMIR5, CLM7 (CMRF-35-like Molecule 7) and TREM5 (Triggering Receptor Expressed on Myeloid cells 5) belongs to a multigene family whose members are closely mapped to a region of human chromosome 17 and mouse chromosome 11. These genes encode type I membrane proteins with a single extracellular Ig-like domain and the proteins are expressed widely in leukocytes. LMIR/CD300 family proteins are immunoregulatory signaling molecules that either have cytoplasmic ITIM motifs or can interact with ITAM motif-bearing molecules through a characteristic transmembrane domain containing positively charged amino acid residues. CD300b/LMIR5 is an ITAM adaptor-associated receptor with a short cytoplasmic region. Human and mouse CD300b/LMIR5 share approximately 45% amino acid sequence identity.

Long Name

Leukocyte Mono Ig-like Receptor 5

Alternate Names

CD300b, CD300LB, CLM7, Gm253, LMIR5, TREM-5

Entrez Gene IDs

124599 (Human); 217304 (Mouse)

Gene Symbol

CD300LB

UniProt

NP_777552

Additional CD300b/LMIR5 Products

Product Documents for Human CD300b/LMIR5 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CD300b/LMIR5 Antibody

For research use only

Citations for Human CD300b/LMIR5 Antibody

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