Human CD300b/LMIR5 Antibody

Catalog # Availability Size / Price Qty
AF2879
AF2879-SP
Detection of CD300b/LMIR5 in Human Monocytes by Flow Cytometry.
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Human CD300b/LMIR5 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD300b/LMIR5 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human LMIR2 and recombinant mouse LMIR5 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD300b/LMIR5
Ile55-His187
Accession # NP_777552
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of CD300b/LMIR5 in Human Monocytes by Flow Cytometry. View Larger

Detection of CD300b/LMIR5 in Human Monocytes by Flow Cytometry. Human whole blood monocytes were stained with Goat Anti-Human CD300b/LMIR5 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF2879, filled histogram) or control antibody (Catalog # AB‑108‑C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD300b/LMIR5

CD300b, also known as LMIR5, CLM7 (CMRF-35-like Molecule 7) and TREM5 (Triggering Receptor Expressed on Myeloid cells 5) belongs to a multigene family whose members are closely mapped to a region of human chromosome 17 and mouse chromosome 11. These genes encode type I membrane proteins with a single extracellular Ig-like domain and the proteins are expressed widely in leukocytes. LMIR/CD300 family proteins are immunoregulatory signaling molecules that either have cytoplasmic ITIM motifs or can interact with ITAM motif-bearing molecules through a characteristic transmembrane domain containing positively charged amino acid residues. CD300b/LMIR5 is an ITAM adaptor-associated receptor with a short cytoplasmic region. Human and mouse CD300b/LMIR5 share approximately 45% amino acid sequence identity.

Long Name
Leukocyte Mono Ig-like Receptor 5
Entrez Gene IDs
124599 (Human); 217304 (Mouse)
Alternate Names
CD300 antigen like family member B; CD300 antigen-like family member B; CD300 molecule-like family member b; CD300b antigen; CD300b; CD300LB; CLM7; CLM-7; CLM7IREM3CD300 molecule like family member b; CMRF35A2; CMRF35-A2; CMRF35-like molecule 7; EC 1.13.11.6; EC 6.3.4.3; Gm253; Immune receptor expressed on myeloid cells 3; IREM-3; Leukocyte mono-Ig-like receptor 5; LMIR5; TREM-5; TREM5CD300b; Triggering receptor expressed on myeloid cells 5

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FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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