Human CD39L3/ENTPD3 Antibody

Catalog # Availability Size / Price Qty
AF4400
AF4400-SP
CD39L3/ENTPD3 in Human Salivary Gland.
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Human CD39L3/ENTPD3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD39L3/ENTPD3 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse CD39L3 is observed, and less than 2% cross-reactivity with recombinant human (rh) CD39L1 and rhCD39 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD39L3/ENTPD3
Gln44-Pro485
Accession # O75355
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human CD39L3/ENTPD3 (Catalog # 4400-EN)
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunohistochemistry CD39L3/ENTPD3 in Human Salivary Gland. View Larger

CD39L3/ENTPD3 in Human Salivary Gland. CD39L3/ENTPD3 was detected in immersion fixed paraffin-embedded sections of human salivary gland using Sheep Anti-Human CD39L3/ENTPD3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4400) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to the plasma membranes of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry CD39L3/ENTPD3 in Human Pancreas. View Larger

CD39L3/ENTPD3 in Human Pancreas. CD39L3/ENTPD3 was detected in immersion fixed paraffin-embedded sections of human pancreas using Sheep Anti-Human CD39L3/ENTPD3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4400) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to pancreatic islets. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD39L3/ENTPD3

Ectonucleoside triphosphate diphosphohydrolase-3 (NTPDase-3), encoded by the ENTPD3 gene and also known as CD39L3, is an integral membrane protein with an extracellular active site (1). Recombinant human NTPDase-3 was expressed as a protein lacking its N- and C-terminal transmembrane domains, resulting in the secretion of the soluble ectodomain. NTPDase-3 hydrolyzes the beta - and gamma -phosphate residues of nucleotides, preferring ATP, ADP, UTP, and UDP as substrates (1). Through its hydrolysis of extracellular nucleotides, NTPDase-3 plays a role in the regulation of purinergic signaling (2). The enzyme is expressed at its highest levels in brain, pancreas, spleen and prostate tissues (3).

References
  1. Lavoie, E.G. et al. (2004) Biochem. Pharmacol. 67:1917.
  2. Crawford, P.A. et al. (2007) Arch. Biochem. Biophys. 457:7.
  3. Chadwick, B.P. and A.M. Frischauf (1998) Genomics 50:357.
Long Name
Ecto-Nucleoside Triphosphate Diphosphohydrolase 3
Entrez Gene IDs
956 (Human); 215446 (Mouse); 316077 (Rat)
Alternate Names
ATPDase; CD39 antigen-like 3; CD39L3; CD39L3EC 3.6.1.5; CD39-like 3; EC 3.6.1; Ecto-apyrase 3; Ecto-ATP diphosphohydrolase 3; Ecto-ATPase 3; Ecto-ATPDase 3; ectonucleoside triphosphate diphosphohydrolase 3; ENTPD3; FLJ93839; HB6; HB6NTPDase 3; NTPDase-3

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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