Human CD4 Alexa Fluor® 594-conjugated Antibody

FAB3791T Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB3791T-100
FAB3791T-025

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Human CD4 Alexa Fluor® 594-conjugated Antibody in Data

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Human CD4 Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD4 in direct ELISAs and Western blots. Does not cross-react with recombinant mouse CD4.

Source

Monoclonal Mouse IgG_2A Clone # 11830

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Recombinant human CD4
Extracellular domain
Accession # P01730

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

5 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry

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Detection of CD4 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A) and either (A) Mouse Anti-Human CD4 Alexa Fluor® 594-conjugated Monoclonal Antibody (Catalog # FAB3791T) or (B) Mouse IgG_2AAlexa Fluor 594 Isotype Control (Catalog # IC003T). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD4

CD4 is a 54-60 kDa type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily. It is expressed predominantly on hematopoietic cells such as T cells, monocytes and Langerhans cells. T cells known to be positive for CD4 include Th1, Th2, Th17, Th22, Th9, Tfh and Treg subsets, plus select thymocytes. CD4 exists as either an oxidized monomer (3 internal disulfide bonds), a reduced monomer (2 internal disulfide bonds) and a disulfide-linked dimer. CD4 functions in collaboration with the T cell receptor in the recognition of peptide antigens that are presented by class II major histocompatibility complexes. In particular, CD4 in its dimeric form binds to the MHC complex at non-polymorphic sites. Monomeric CD4 has also been shown to be a coreceptor for HIV entry and specifically to bind gp120, the external envelope glycoprotein of HIV. Over amino acids (aa) 26-396 (the extracellular domain), human and mouse CD4 share 58% aa sequence identity.

Entrez Gene IDs

920 (Human); 12504 (Mouse); 24932 (Rat); 403931 (Canine); 101864991 (Cynomolgus Monkey); 493775 (Feline)

Alternate Names

CD_antigen: CD4; CD4 antigen (p55); CD4 antigen; CD4 molecule; CD4 receptor; CD4; CD4mut; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4