Human CD4 Alexa Fluor® 594-conjugated Antibody

    
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  • Species Reactivity
    Human
  • Specificity
    Detects human CD4 in direct ELISAs and Western blots. Does not cross-react with recombinant mouse CD4.
  • Source
    Monoclonal Mouse IgG2A Clone # 11830
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Recombinant human CD4
    Extracellular domain
    Accession # P01730
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 594
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of CD4 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD3 epsilon APC‑conjugated Monoclonal Antibody (Catalog # FAB100A) and either (A) Mouse Anti-Human CD4 Alexa Fluor® 594‑conjugated Monoclonal Antibody (Catalog # FAB3791T) or (B) Mouse IgG2A Alexa Fluor 594 Isotype Control (Catalog # IC003T). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD4
CD4 is a 54-60 kDa type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily. It is expressed predominantly on hematopoietic cells such as T cells, monocytes and Langerhans cells. T cells known to be positive for CD4 include Th1, Th2, Th17, Th22, Th9, Tfh and Treg subsets, plus select thymocytes. CD4 exists as either an oxidized monomer (3 internal disulfide bonds), a reduced monomer (2 internal disulfide bonds) and a disulfide-linked dimer. CD4 functions in collaboration with the T cell receptor in the recognition of peptide antigens that are presented by class II major histocompatibility complexes. In particular, CD4 in its dimeric form binds to the MHC complex at non-polymorphic sites. Monomeric CD4 has also been shown to be a coreceptor for HIV entry and specifically to bind gp120, the external envelope glycoprotein of HIV. Over amino acids (aa) 26-396 (the extracellular domain), human and mouse CD4 share 58% aa sequence identity.
  • Entrez Gene IDs:
    920 (Human); 12504 (Mouse); 24932 (Rat); 403931 (Canine); 493775 (Feline)
  • Alternate Names:
    CD4 antigen (p55); CD4 antigen; CD4 molecule; CD4 receptor; CD4; CD4mut; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4
Related Research Areas

FAQs

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Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A Alexa Fluor® 594-conjugated Isotype Control

Ctrl IC003T
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 7
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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