Human CD4 Alexa Fluor® 750-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB3791S-100
FAB3791S-025
Detection  of CD4 in Human Blood Monocytes by Flow Cytometry.
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Human CD4 Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD4 in direct ELISAs and Western blots. Does not cross-react with recombinant mouse CD4.
Source
Monoclonal Mouse IgG2A Clone # 11830
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Recombinant human CD4
Extracellular domain
Accession # P01730
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 750

Applications

Recommended Concentration
Sample
Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection  of CD4 in Human Blood Monocytes by Flow Cytometry. View Larger

Detection of CD4 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD3 epsilon PE‑conjugated Monoclonal Antibody (Catalog # FAB100P) and either (A) Mouse Anti-Human CD4 Alexa Fluor® 750‑conjugated Monoclonal Antibody (Catalog # FAB3791S) or (B) Mouse IgG2A Alexa Fluor 750 Isotype Control (Catalog # IC003S). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD4

CD4 is a 54-60 kDa type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily. It is expressed predominantly on hematopoietic cells such as T cells, monocytes and Langerhans cells. T cells known to be positive for CD4 include Th1, Th2, Th17, Th22, Th9, Tfh and Treg subsets, plus select thymocytes. CD4 exists as either an oxidized monomer (3 internal disulfide bonds), a reduced monomer (2 internal disulfide bonds) and a disulfide-linked dimer. CD4 functions in collaboration with the T cell receptor in the recognition of peptide antigens that are presented by class II major histocompatibility complexes. In particular, CD4 in its dimeric form binds to the MHC complex at non-polymorphic sites. Monomeric CD4 has also been shown to be a coreceptor for HIV entry and specifically to bind gp120, the external envelope glycoprotein of HIV. Over amino acids (aa) 26-396 (the extracellular domain), human and mouse CD4 share 58% aa sequence identity.

Entrez Gene IDs
920 (Human); 12504 (Mouse); 24932 (Rat); 403931 (Canine); 493775 (Feline)
Alternate Names
CD4 antigen (p55); CD4 antigen; CD4 molecule; CD4 receptor; CD4; CD4mut; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4

Product Datasheets

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