Human CD47 DuoSet ELISA

Human CD47 DuoSet ELISA Summary

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Product Features

• Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
• Development protocols are provided to guide further assay optimization
• Assay can be customized to your specific needs
• Economical alternative to complete kits

Kit Content

• Capture Antibody
• Detection Antibody
• Recombinant Standard
• Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

Data Example

View Larger

Human CD47 ELISA Standard Curve

Product Datasheets

Preparation and Storage

Background: CD47

CD47, also known as Integrin-Associated Protein (IAP) and OA3, is a ubiquitous transmembrane protein that binds to SIRP family members on macrophages, neutrophils, and T cells. These interactions prevent macrophage-mediated clearance of healthy CD47-expressing cells and promote immune cell transmigration across the vascular endothelium. CD47 associates in cis with Fas on T cells and enhances Fas-mediated apoptosis; its ligation promotes T cell anergy and dampens Th1 immune responses. CD47 also associates in cis with several Integrins?which can positively or negatively modulate Integrin-mediated function. CD47 binding by Thrombospondin-1 inhibits the angiogenic and vasorelaxant effects of nitric oxide. On dendritic cells and myeloma cells, CD47 ligation by TSP-1 induces giant cell formation and osteoclast differentiation.