Key Product Details

Species Reactivity

Human

Applications

Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2664C
View all CD81 Primary Antibodies »

Product Specifications

Immunogen

CHO-derived human CD81 protein
Phe113-Ala201
Accession # P60033

Specificity

Detects human CD81 in Elisas.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human CD81 Antibody

Detection of Human CD81 by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line and Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 4 µg/mL of Rabbit Anti-Human CD81 Monoclonal Antibody (Catalog # MAB46152) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for CD81 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of Human CD81 by Simple WesternTM.

Simple Western shows lysates of Exosome Standards (HT-29) (NBP3-11685), Exosome Standards (LNCaP) (NBP3-11687) and SK‑Mel‑28 human malignant melanoma cell line, loaded at 0.5 mg/ml. A specific band was detected for CD81 at approximately 29 kDa (as indicated) using 20 µg/mL of Rabbit Anti-Human CD81 Monoclonal Antibody (Catalog # MAB46152). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Human CD81 by Simple WesternTM.

Simple Western lane view shows lysates of SK‑Mel‑28 human malignant melanoma cell line, loaded at 0.2 mg/mL. A specific band was detected for CD81 at approximately 27 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human CD81 Monoclonal Antibody (Catalog # MAB46152). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Human CD81 by Simple Western

(C) Detection of exosome marker proteins in nanoparticle-enriched fluid after separation by TFF + BIA Strong AEX and Exodus using Wes Protein Simple. "M" denotes Marker, "1" represents mixed original sample 1 of hUC-MSC-0214, "2" represents exosome-enriched fluid after separation by TFF + BIA-EV, "3" represents mixed original sample 2 of hUC-MSC-0214, and "4" represents nanoparticle-enriched fluid after separation by Exodus. Exosome-specific positively expressed proteins include CD9, CD63, CD81, and TSG101. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/40496140), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human CD81 Antibody

Application
Recommended Usage

Simple Western

20 µg/mL
Sample: Exosome Standards (HT-29) (Catalog # NBP3-11685), Exosome Standards (LnCAP) (Catalog # NBP3-11687), and SK-Mel-28 human malignant melanoma cell line

Western Blot

4 µg/mL
Sample: Jurkat human acute T cell leukemia cell line and Ramos human Burkitt's lymphoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: CD81

CD81, also known as TAPA-1 and Tetraspanin-28, is an approximately 25 kDa palmitoylated component of plasma membrane lipid rafts (1). It contains four transmembrane segments, two extracellular loops of 30 and 90 amino acids (aa), and three short cytoplasmic regions (2, 3). Within the large extracellular loop, human CD81 shares 90% and 84% aa sequence identity with mouse and rat CD81, respectively. CD81 associates with a wide range of membrane proteins including CD151, TfR2, LDL R, PCSK9, Glypican 3, IFITM1, IGSF8/CD316, FPRP, and complexes of CD19-CD21 (4-11). It is required for the development of CD4+CD8+ DP thymocytes (12) and hepatocyte infection by Plasmodium sporozoites (13). It also supports B cell receptor signaling (11), Hepcidin expression (5), monocyte and B cell tethering to the vascular endothelium (14), and the immunosuppressive function of Treg and MDSC (15). CD81 additionally functions as a receptor for the E2 glycoprotein of hepatitis C virus (16). The CD81-E2 interaction inhibits NK cell cytolytic activity, provides a co-stimulatory signal to T cells, and inhibits the maturation of plasmacytoid dendritic cells (17-19).

References

  1. Charrin, S. et al. (2014) J. Cell Sci. 127:3641.
  2. Oren, R. et al. (1990) Mol. Cell. Biol. 10:4007.
  3. Levy, S. et al. (1991) J. Biol. Chem. 266:14597.
  4. Zhu, Y.-Z. et al. (2012) Virology 429:112.
  5. Chen, J. and C.A. Enns (2015) J. Biol. Chem. 290:7841.
  6. Le, Q.-T. et al. (2015) J. Biol. Chem. 290:23385.
  7. Takahashi, S. et al. (1990) J. Immunol. 145:2207.
  8. Stipp, C.S. et al. (2001) J. Biol. Chem. 276:40545.
  9. Stipp, C.S. et al. (2001) J. Biol. Chem. 276:4853.
  10. Liu, B. et al. (2009) Am. J. Pathol. 175:717.
  11. Cherukuri, A. et al. (2004) J. Immunol. 279:31973.
  12. Boismenu, R. et al. (1996) Science 271:198.
  13. Silvie, O. et al. (2003) Nat. Med. 9:93.
  14. Feigelson, S.W. et al. (2003) J. Biol. Chem. 278:51203.
  15. Vences-Catalan, F. et al. (2015) Cancer Res. 75:4517.
  16. Pileri, P. et al. (1998) Science 282:938.
  17. Crotta, S. et al. (2002) J. Exp. Med. 195:35.
  18. Tseng, C.-T. and G.R. Klimpel (2002) J. Exp. Med. 195:43.
  19. Tu, Z. et al. (2013) Cell. Immunol. 284:98.

Alternate Names

CD81, TAPA-1, TSPAN28

Entrez Gene IDs

975 (Human); 12520 (Mouse); 25621 (Rat)

Gene Symbol

CD81

UniProt

P60033

Additional CD81 Products

Product Documents for Human CD81 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human CD81 Antibody

For research use only

Citations for Human CD81 Antibody

Customer Reviews for Human CD81 Antibody

There are currently no reviews for this product. Be the first to review Human CD81 Antibody and earn rewards!

Have you used Human CD81 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies