Human CDCP1 Antibody (309137)

R&D Systems | Catalog # MAB2666

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Immunohistochemistry, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 309137
View all CDCP1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CDCP1 isoform 1
Ala33-Leu666
Accession # NP_073753

Specificity

Detects human CDCP1 in direct ELISAs and Western blots. Recognizes an epitope found between aa 33‑369 of human CDCP1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human CDCP1 Antibody (309137)

Detection of CDCP1 by Western Blot

Detection of CDCP1 by Western Blot

Co-overexpression of Src and CDCP1 leads to focus formation.(A) Fifty thousand NIH3T3 cells were infected with 500.000 retroviruses encoding CDCP1 or Src individually or together. After 48 h the cells were reseeded in a 10 cm-dish and grown for 3 weeks, with 3 media changes per week. In parallel, a 2% aliquot was taken from the CDCP1/Src infected cells and reseeded together with 150.000 parental NIH3T3 cells (1∶50 dilution). Finally, the cells were stained with crystal violett. (B) Parental NIH3T3 cells, cells infected with the CDCP1 encoding retrovirus or individual foci derived from infections at lower m.o.i. than in A that had been isolated and expanded, were lysed, aliquots with similar amounts of protein run on an SDS-PAGE, proteins transferred to nitrocellulose and the filter blotted with the indicated antibodies. alpha PY, anti-phosphotyrosine. Size markers (kDa) are indicated. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/23300860), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human CDCP1 Antibody (309137)

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human CDCP1, see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Recombinant Human CDCP1

Reviewed Applications

Read 1 review rated 4 using MAB2666 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CDCP1

CDCP1, also known as SIMA135, is a novel 140 kDa type I transmembrane glycoprotein with three CUB protein-protein interaction domains in its 635 aa extracellular region. The 148 aa cytoplasmic region contains canonical phosphorylation sites for Src kinase family members and binding sites for SH3 domains. By alternative splicing, a secreted 310 aa residue form of CDCP1 also exists. The amino-terminal region of approximately 265 aa of the type I membrane CDCP1 can also be proteolytically cleaved. CDCP1 is found on the surface of epithelial and bone marrow-derived stem cells. The extracellular region of human CDCP1 shares 84% aa identity with that of the mouse protein.

Long Name

CUB Domain Containing Protein 1

Alternate Names

CD318, SIMA135

Entrez Gene IDs

64866 (Human); 109332 (Mouse)

Gene Symbol

CDCP1

UniProt

NP_073753

Additional CDCP1 Products

Product Documents for Human CDCP1 Antibody (309137)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CDCP1 Antibody (309137)

For research use only

Citations for Human CDCP1 Antibody (309137)

Customer Reviews for Human CDCP1 Antibody (309137) (1)

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 23300860
    Species: Human
    Verified Customer | Posted 02/19/2015

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