Human CEACAM-4 Antibody

Catalog # Availability Size / Price Qty
Detection of Human CEACAM‑4 by Western Blot.
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Product Details
Supplemental Products

Human CEACAM-4 Antibody Summary

Species Reactivity
Detects human CEACAM‑4 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human CEACAM-1, -3, -5, -6, -7, or -8 is observed.
Monoclonal Mouse IgG1 Clone # 568410
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human CEACAM-4
Accession # O75871
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
2 µg/mL
See below
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human CEACAM-4 antibody by Western Blot. View Larger

Detection of Human CEACAM‑4 by Western Blot. Western blot shows lysates of HL-60 human acute promyelocytic leukemia cell line, THP-1 human acute monocytic leukemia cell line, IMR-90 human lung fibroblast cell line, NCI-H596 human lung adenosquamous carcinoma cell line, and human lung cancer tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CEACAM-4 Monoclonal Antibody (Catalog # MAB4354) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CEACAM-4 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry CEACAM-4 antibody in Human Lung Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

CEACAM‑4 in Human Lung Cancer Tissue. CEACAM-4 was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Mouse Anti-Human CEACAM-4 Monoclonal Antibody (Catalog # MAB4354) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CEACAM-4

Carcinoembryonic antigen cell adhesion molecule 4 (CEACAM-4), also known as CGM7 and W236, is a 40 kDa transmembrane glycoprotein member of the CEACAM family of proteins. Its 209 amino acid extracellular domain contains one Ig-like V-type domain. CEACAM-4 is expressed on granulocytes as well as on B and T cell lymphoblastic leukemias. Mouse and rat orthologs of CEACAM-4 have not been described.

Long Name
Carcinoembryonic Antigen-related Cell Adhesion Molecule 4
Entrez Gene IDs
1089 (Human)
Alternate Names
carcinoembryonic antigen CGM7; carcinoembryonic antigen gene family member 7; carcinoembryonic antigen-related cell adhesion molecule 4; CEACAM4; CEACAM-4; CGM7; CGM7_HUMAN

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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