Human CEACAM-4 Antibody

Catalog # Availability Size / Price Qty
MAB7845
MAB7845-SP
Detection of CEACAM‑4 in THP‑1 Human Cell Line by Flow Cytometry.
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Human CEACAM-4 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CEACAM-4 in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 822608
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human CEACAM-4
Phe36-Val140
Accession # O75871
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of CEACAM-4 antibody in THP-1 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of CEACAM‑4 in THP‑1 Human Cell Line by Flow Cytometry. THP-1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human CEACAM-4 Monoclonal Antibody (Catalog # MAB7845, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CEACAM-4

CEACAMs are intercellular adhesion molecules that form Ca2+-dependent homo- and heterotypic interactions, and typically serve as pathogen receptors. All CEACAMs are heavily glycosylated and contain one N-terminal V-type Ig-like domain. CEACAMs also exhibit variability in their structure by containing transmembrane domains (#CEACAM-1, 3 & 4), GPI-linked domains (#5, 6, 7, & 8), or CD66 designations (#1, 3, 5, 6, & 7).

Long Name
Carcinoembryonic Antigen-related Cell Adhesion Molecule 4
Entrez Gene IDs
1089 (Human)
Alternate Names
carcinoembryonic antigen CGM7; carcinoembryonic antigen gene family member 7; carcinoembryonic antigen-related cell adhesion molecule 4; CEACAM4; CEACAM-4; CGM7; CGM7_HUMAN

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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