Human CEACAM-4 Antibody (822608)

R&D Systems | Catalog # MAB7845

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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 822608
View all CEACAM-4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CEACAM-4
Phe36-Val140
Accession # O75871

Specificity

Detects human CEACAM-4 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human CEACAM-4 Antibody (822608)

Detection of CEACAM-4 antibody in THP-1 Human Cell Line antibody by Flow Cytometry.

Detection of CEACAM‑4 in THP‑1 Human Cell Line by Flow Cytometry.

THP-1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human CEACAM-4 Monoclonal Antibody (Catalog # MAB7845, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Applications for Human CEACAM-4 Antibody (822608)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: THP‑1 human acute monocytic leukemia cell line

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CEACAM-4

CEACAMs are intercellular adhesion molecules that form Ca2+-dependent homo- and heterotypic interactions, and typically serve as pathogen receptors. All CEACAMs are heavily glycosylated and contain one N-terminal V-type Ig-like domain. CEACAMs also exhibit variability in their structure by containing transmembrane domains (#CEACAM-1, 3 & 4), GPI-linked domains (#5, 6, 7, & 8), or CD66 designations (#1, 3, 5, 6, & 7).

Long Name

Carcinoembryonic Antigen-related Cell Adhesion Molecule 4

Alternate Names

CEACAM4, CGM7

Entrez Gene IDs

1089 (Human)

Gene Symbol

CEACAM4

UniProt

O75871

Additional CEACAM-4 Products

Product Documents for Human CEACAM-4 Antibody (822608)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CEACAM-4 Antibody (822608)

For research use only

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