Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all CHD7 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CHD7
Ala263-Gln457
Accession # Q9P2D1

Specificity

Detects human CHD7 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human CHD7 Antibody

Detection of Human CHD7 antibody by Western Blot.

Detection of Human CHD7 by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human CHD7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7350) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CHD7 at approximately 350 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

CHD7 antibody in HepG2 Human Cell Line by Immunocytochemistry (ICC).

CHD7 in HepG2 Human Cell Line.

CHD7 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Sheep Anti-Human CHD7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7350) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of CHD7 by Western Blot

Detection of CHD7 by Western Blot

CHD7 and its ATP-dependent chromatin remodeling activities are required for sensory epithelium derivation. A Sanger sequencing chromatograms of CHD7KO/+ and CHD7KO/KO alleles cloned into TOPO vectors. b Western blotting of WT (PAX2nG), CHD7KO/+, and CHD7KO/KO hESCs using an anti-CHD7 antibody. Source data are provided as a Source Data file. c Sanger sequencing chromatograms of WT (PAX2nG), CHD7S834F/+, and CHD7S834F/S834F hESCs at the CHD7 c.2501 locus. d–w Immunostaining of d20 and d70 WT and CHD7 mutant organoids. Antibodies highlight otic progenitors (PAX2nG, PAX8, and EPCAM), hair cells (MYO7A, POU4F3, PCP4, SOX2, and F-actin for stereocilia of hair cells), and supporting cells (SPARCL1 and SOX2). Scale bars, 25 µm (top two rows of d–w), 10 µm (third row of d–w), and 5 µm (bottom row of d–w). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36396635), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of CHD7 by Western Blot

Detection of CHD7 by Western Blot

CHD7 is expressed at key otic development stages.a Western blotting of WT (PAX2−2a-nGFP cell line, hereafter PAX2nG) and CHD7−3×Flag hESCs using anti-Flag and anti-CHD7 antibodies. Calculated molecular weight of CHD7 and CHD7-3×Flag are 336 kDa and 339 kDa, respectively. Source data are provided as a Source Data file. b Schematics of otic lineage differentiation during human inner ear organoid culture. Schematics adapted from Nie J, Hashino E. (2020) Generation of inner ear organoids from human pluripotent stem cells. Methods in Cell Biology, 159: 303–321, with permission from Elsevier. c–g Immunostaining at key otic development stages in CHD7−3×Flag PAX2nG human inner ear organoids using an anti-Flag antibody, as well as antibodies against NNE markers TFAP2A and CDH1, OEPD markers TFAP2A and PAX8, otic placode/pit and otic vesicle markers PAX2nG, PAX8, and EPCAM, and hair cell markers MYO7A and SOX2 and supporting cell marker SOX2. Scale bars, 25 µm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36396635), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of CHD7 by Western Blot

Detection of CHD7 by Western Blot

CHD7 and its ATP-dependent chromatin remodeling activities are required for sensory epithelium derivation. A Sanger sequencing chromatograms of CHD7KO/+ and CHD7KO/KO alleles cloned into TOPO vectors. b Western blotting of WT (PAX2nG), CHD7KO/+, and CHD7KO/KO hESCs using an anti-CHD7 antibody. Source data are provided as a Source Data file. c Sanger sequencing chromatograms of WT (PAX2nG), CHD7S834F/+, and CHD7S834F/S834F hESCs at the CHD7 c.2501 locus. d–w Immunostaining of d20 and d70 WT and CHD7 mutant organoids. Antibodies highlight otic progenitors (PAX2nG, PAX8, and EPCAM), hair cells (MYO7A, POU4F3, PCP4, SOX2, and F-actin for stereocilia of hair cells), and supporting cells (SPARCL1 and SOX2). Scale bars, 25 µm (top two rows of d–w), 10 µm (third row of d–w), and 5 µm (bottom row of d–w). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36396635), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of CHD7 by Western Blot

Detection of CHD7 by Western Blot

CHD7 is expressed at key otic development stages.a Western blotting of WT (PAX2−2a-nGFP cell line, hereafter PAX2nG) and CHD7−3×Flag hESCs using anti-Flag and anti-CHD7 antibodies. Calculated molecular weight of CHD7 and CHD7-3×Flag are 336 kDa and 339 kDa, respectively. Source data are provided as a Source Data file. b Schematics of otic lineage differentiation during human inner ear organoid culture. Schematics adapted from Nie J, Hashino E. (2020) Generation of inner ear organoids from human pluripotent stem cells. Methods in Cell Biology, 159: 303–321, with permission from Elsevier. c–g Immunostaining at key otic development stages in CHD7−3×Flag PAX2nG human inner ear organoids using an anti-Flag antibody, as well as antibodies against NNE markers TFAP2A and CDH1, OEPD markers TFAP2A and PAX8, otic placode/pit and otic vesicle markers PAX2nG, PAX8, and EPCAM, and hair cell markers MYO7A and SOX2 and supporting cell marker SOX2. Scale bars, 25 µm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36396635), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human CHD7 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed HepG2 human hepatocellular carcinoma cell line

Western Blot

0.5 µg/mL
Sample: Jurkat human acute T cell leukemia cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CHD7

CHD7 (Chromohelicase/ATPase DNA-binding protein 7) is a 350 kDa member of the third subgroup in the SNF2/RAD54 helicase family of proteins. It is an ATP‑dependent chromatin remodeling factor that also binds histones (H3 at Lys4) and influences transcription. CHD7 binds more that 10,000 sites on chromatin, particularly in locations associated with gene enhancement. Notably, CHD7 is most associated with the nervous system, and is found in embryonic hypothalamus (GnRH neurons), olfactory epithelium and spinal cord, and adult preoptic hypothalamus plus hippocampus. Human CHD7 is 2997 amino acids (aa) in length. It contains consecutive Gln-, Pro- and Lys-rich regions (aa 151-718), two Chromo (chromatin-organizer-modifer) domains (aa 800-947), a helicase ATP-binding domain (aa 980-1154), a C-terminal helicase domain (aa 1294-1464), one coiled-coil region (aa 2410-2431) and two BRK domains (aa 2562-2604 and 2642-2686). There are at least seven utilized Ser/Thr phosphorylation sites, and three potential isoform variants. One isoform contains a 12 aa substitution for aa 1127-2997, a second 145 kDa isoform shows a deletion of aa 573-2621, while a third isoform contains a 15 aa insertion after Ser698. CHD7 is reported to bind to subgroup three member CHD8. Over aa 263-457, human CHD7 shares 96% aa identity with mouse CHD7.

Long Name

Chromodomain Helicase DNA Binding Protein 7

Alternate Names

ATP-dependent helicase CHD7, CHD-7, chromodomain helicase DNA binding protein 7, chromodomain helicase DNA binding protein 7 isoform CRA_e, chromodomain-helicase-DNA-binding protein 7, EC 3.6.1, EC 3.6.4.12, FLJ20357, FLJ20361, IS3, KIAA1416KAL5

Entrez Gene IDs

55636 (Human); 320790 (Mouse); 312974 (Rat)

Gene Symbol

CHD7

UniProt

Q9P2D1 (Human)

Additional CHD7 Products

Product Documents for Human CHD7 Antibody

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Product Specific Notices for Human CHD7 Antibody

For research use only

Citations for Human CHD7 Antibody

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Protocols

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