Human CHD7 Antibody

Catalog # Availability Size / Price Qty
AF7350
AF7350-SP
Detection of Human CHD7 by Western Blot.
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Human CHD7 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CHD7 in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human CHD7
Ala263-Gln457
Accession # Q9P2D1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human CHD7 by Western Blot. View Larger

Detection of Human CHD7 by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human CHD7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7350) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CHD7 at approximately 350 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunocytochemistry CHD7 in HepG2 Human Cell Line. View Larger

CHD7 in HepG2 Human Cell Line.

CHD7 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Sheep Anti-Human CHD7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7350) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CHD7

CHD7 (Chromohelicase/ATPase DNA-binding protein 7) is a 350 kDa member of the third subgroup in the SNF2/RAD54 helicase family of proteins. It is an ATP‑dependent chromatin remodeling factor that also binds histones (H3 at Lys4) and influences transcription. CHD7 binds more that 10,000 sites on chromatin, particularly in locations associated with gene enhancement. Notably, CHD7 is most associated with the nervous system, and is found in embryonic hypothalamus (GnRH neurons), olfactory epithelium and spinal cord, and adult preoptic hypothalamus plus hippocampus. Human CHD7 is 2997 amino acids (aa) in length. It contains consecutive Gln-, Pro- and Lys-rich regions (aa 151-718), two Chromo (chromatin-organizer-modifer) domains (aa 800-947), a helicase ATP-binding domain (aa 980-1154), a C-terminal helicase domain (aa 1294-1464), one coiled-coil region (aa 2410-2431) and two BRK domains (aa 2562-2604 and 2642-2686). There are at least seven utilized Ser/Thr phosphorylation sites, and three potential isoform variants. One isoform contains a 12 aa substitution for aa 1127-2997, a second 145 kDa isoform shows a deletion of aa 573-2621, while a third isoform contains a 15 aa insertion after Ser698. CHD7 is reported to bind to subgroup three member CHD8. Over aa 263-457, human CHD7 shares 96% aa identity with mouse CHD7.

Long Name
Chromodomain Helicase DNA Binding Protein 7
Entrez Gene IDs
55636 (Human); 320790 (Mouse); 312974 (Rat)
Alternate Names
ATP-dependent helicase CHD7; CHD7; CHD-7; chromodomain helicase DNA binding protein 7 isoform CRA_e; chromodomain helicase DNA binding protein 7; chromodomain-helicase-DNA-binding protein 7; EC 3.6.1; EC 3.6.4.12; FLJ20357; FLJ20361; IS3; KIAA1416KAL5

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