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Human Chitobiase/CTBS Antibody

Catalog #: MAB4829 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB4829
MAB4829-SP

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Human Chitobiase/CTBS Antibody Summary

Species Reactivity
Human
Specificity
Detects human Chitobiase/CTBS in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 486725
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Chitobiase/CTBS
Thr39-Arg385
Accession # Q01459
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Chitobiase/CTBS
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Chitobiase/CTBS, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Chitobiase/CTBS

Di-N-acetyl-chitobiase (CTBS) is a lysosomal glycosidase that is involved in the degradation of asparagine-linked glycoproteins. The amino acid sequence of human CTBS shares 99%, 86%, 82%, and 77% identity with that of chimpanzee, mouse, rat, and canine CTBS, respectively.

Entrez Gene IDs
1486 (Human); 74245 (Mouse); 81652 (Rat)
Alternate Names
Chitobiase; chitobiase, di-N-acetyl-; CTBdi-N-acetylchitobiase; CTBS; EC 3.2.1.-

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