Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Chondroadherin Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant Chondroadherin
Cys23-His359
Accession # O15335

Specificity

Detects human Chondroadherin in direct ELISAs and Western Blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Chondroadherin Antibody

Detection of Human Chondroadherin antibody by Western Blot.

Detection of Human Chondroadherin by Western Blot.

Western blot shows lysates of human cartilage tissue. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human Chondroadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8218) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Chondroadherin at approximately 34-36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Chondroadherin antibody in Human Cartilage by Immunohistochemistry (IHC-P).

Chondroadherin in Human Cartilage.

Chondroadherin was detected in immersion fixed paraffin-embedded sections of human cartilage using Sheep Anti-Human Chondroadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8218) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of chondrocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Chondroadherin Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human cartilage

Western Blot

2 µg/mL
Sample: Human cartilage tissue

Reviewed Applications

Read 1 review rated 4 using AF8218 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Chondroadherin

Chondroadherin is an approximately 36 kDa secreted glycosaminoglycan that contains nine tandem leucine-rich repeats as well as N-terminal and C-terminal leucine-rich domains. It is a component of the extracellular matrix in cartilage, tendon, bony growth plates, skeletal and cardiac muscle, lung, gastrointestinal tract, pancreas, cerebellum, and eye. It is cleaved by HTRA1/Omi during intervertebral disc degeneration. Chondroadherin functions as an adhesion protein by binding to Integrin a2/b1, Collagen II, and Collagen VI. It also promotes complement activation through binding to C1q and Complement Factor H. Mature human Chondroadherin shares 94% amino acid sequence identity with mouse and rat Chondroadherin.

Alternate Names

CHAD, SLRR4A

Entrez Gene IDs

1101 (Human); 12643 (Mouse); 29195 (Rat)

Gene Symbol

CHAD

UniProt

O15335

Additional Chondroadherin Products

Product Documents for Human Chondroadherin Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Chondroadherin Antibody

For research use only

Customer Reviews for Human Chondroadherin Antibody (1)

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  • Human Chondroadherin Antibody
    Name: Mohamad Youness
    Application: Immunohistochemistry-Frozen
    Sample Tested: cardiac tissue
    Species: Pig
    Verified Customer | Posted 08/01/2023
    Immunofluorescence staining of CHAD (green; 1:100) on pig cardiac tissue after infarction. Blue represents nuclei (DAPI)
    Human Chondroadherin Antibody AF8218
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

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Protocols

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