Detection of Human Chondroadherin by Western Blot.
Western blot shows lysates of human cartilage tissue. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human Chondroadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8218) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Chondroadherin at approximately 34-36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Chondroadherin in Human Cartilage.
Chondroadherin was detected in immersion fixed paraffin-embedded sections of human cartilage using Sheep Anti-Human Chondroadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8218) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of chondrocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Chondroadherin is an approximately 36 kDa secreted glycosaminoglycan that contains nine tandem leucine-rich repeats as well as N-terminal and C-terminal leucine-rich domains. It is a component of the extracellular matrix in cartilage, tendon, bony growth plates, skeletal and cardiac muscle, lung, gastrointestinal tract, pancreas, cerebellum, and eye. It is cleaved by HTRA1/Omi during intervertebral disc degeneration. Chondroadherin functions as an adhesion protein by binding to Integrin a2/b1, Collagen II, and Collagen VI. It also promotes complement activation through binding to C1q and Complement Factor H. Mature human Chondroadherin shares 94% amino acid sequence identity with mouse and rat Chondroadherin.
Entrez Gene IDs:
1101 (Human); 12643 (Mouse); 29195 (Rat)
CHAD; chondroadherin proteoglycan; Chondroadherin; SLRR4A; SLRR4ACartilage leucine-rich protein
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