Human Clathrin Heavy Chain 2/CHC22 Antibody AF6948: R&D Systems

Human Clathrin Heavy Chain 2/CHC22 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Clathrin Heavy Chain 2/CHC22 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human CHC17 is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human Clathrin Heavy Chain 2/CHC22
    Trp1521-Glu1640
    Accession # P53675
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Clathrin Heavy Chain 2/CHC22 by Western Blot. Western blot shows lysates of human testis tissue and U2OS human osteosarcoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Clathrin Heavy Chain 2/CHC22 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6948) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Clathrin Heavy Chain 2/CHC22 at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
Clathrin Heavy Chain 2/CHC22 in Human Mesenchymal Stem Cells. Clathrin Heavy Chain 2/CHC22 was detected in immersion fixed human mesenchymal stem cells differentiated to adipocytes with Human/Mouse/Rat StemXVivo Adipogenic Supplement (Catalog # CCM011) using Sheep Anti-Human Clathrin Heavy Chain 2/CHC22 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6948) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Clathrin Heavy Chain 2/CHC22

CHC22 (clathrin heavy chain on chromosome 22; also Clathrin Heavy Chain 2, CHL-22, CLTCL1, and CLTD) is a 170-190 kDa member of the clathrin heavy chain family of molecules. It is expressed in striated (skeletal and cardiac) muscle and adipocytes. CHC22 appears to participate in glucose transport by interacting with GLUT4 and GGA2, thus contributing to the formation of the GLUT4 storage compartment. CHC22 forms homotrimers, but does not interact with clathrin light chains. Human clathrin heavy chain 2/CHC22 is 1640 amino acids (aa) in length. It contains an N-terminal domain that mediates protein-protein interactions (aa 2-479), a flexible linker region (aa 480-523), a distal segment (aa 524-634), a "proximal" region (aa 639-1640), and a trimerization domain that mediates the formation of a non‑covalent structure (aa 1551-1640). Multiple utilized phosphorylation and acetylation sites exist. There are three potential splice forms. One possesses a seven aa substitution for aa 1620-1640, a second shows a deletion of aa 1479-1535, and a third contains a combination of the afore-mentioned variations. Over aa 1521‑1640, human CHC22 shares 60% aa identity with canine CHC22 and 73% aa identity with human CHC17. In rodent, CHC22 is not expressed as it is represented by a pseudogene.

  • Long Name:
    Clathrin Heavy Chain on Chromosome 22
  • Entrez Gene IDs:
    8218 (Human); 98970 (Mouse)
  • Alternate Names:
    CHC22; Clathrin Heavy Chain 2; clathrin heavy chain on chromosome 22; clathrin, heavy chain-like 1; Clathrin, heavy polypeptide D; CLH-22; CLTCL; CLTCL1; CLTD; heavy polypeptide-like 1
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