Human Clathrin Heavy Chain 2/CHC22 Antibody

Catalog # Availability Size / Price Qty
AF6948
AF6948-SP
Detection of Human Clathrin Heavy Chain 2/CHC22 by Western Blot.
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Product Details
Citations (1)
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Human Clathrin Heavy Chain 2/CHC22 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Clathrin Heavy Chain 2/CHC22 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human CHC17 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Clathrin Heavy Chain 2/CHC22
Trp1521-Glu1640
Accession # P53675
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Clathrin Heavy Chain 2/CHC22 by Western Blot. View Larger

Detection of Human Clathrin Heavy Chain 2/CHC22 by Western Blot. Western blot shows lysates of human testis tissue and U2OS human osteosarcoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Clathrin Heavy Chain 2/CHC22 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6948) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Clathrin Heavy Chain 2/CHC22 at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Clathrin Heavy Chain 2/CHC22 in Human Mesenchymal Stem Cells. View Larger

Clathrin Heavy Chain 2/CHC22 in Human Mesenchymal Stem Cells. Clathrin Heavy Chain 2/CHC22 was detected in immersion fixed human mesenchymal stem cells differentiated to adipocytes with Human/Mouse/Rat StemXVivo Adipogenic Supplement (Catalog # CCM011) using Sheep Anti-Human Clathrin Heavy Chain 2/CHC22 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6948) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Clathrin Heavy Chain 2/CHC22

CHC22 (clathrin heavy chain on chromosome 22; also Clathrin Heavy Chain 2, CHL-22, CLTCL1, and CLTD) is a 170-190 kDa member of the clathrin heavy chain family of molecules. It is expressed in striated (skeletal and cardiac) muscle and adipocytes. CHC22 appears to participate in glucose transport by interacting with GLUT4 and GGA2, thus contributing to the formation of the GLUT4 storage compartment. CHC22 forms homotrimers, but does not interact with clathrin light chains. Human clathrin heavy chain 2/CHC22 is 1640 amino acids (aa) in length. It contains an N-terminal domain that mediates protein-protein interactions (aa 2-479), a flexible linker region (aa 480-523), a distal segment (aa 524-634), a "proximal" region (aa 639-1640), and a trimerization domain that mediates the formation of a non‑covalent structure (aa 1551-1640). Multiple utilized phosphorylation and acetylation sites exist. There are three potential splice forms. One possesses a seven aa substitution for aa 1620-1640, a second shows a deletion of aa 1479-1535, and a third contains a combination of the afore-mentioned variations. Over aa 1521‑1640, human CHC22 shares 60% aa identity with canine CHC22 and 73% aa identity with human CHC17. In rodent, CHC22 is not expressed as it is represented by a pseudogene.

Long Name
Clathrin Heavy Chain on Chromosome 22
Entrez Gene IDs
8218 (Human); 98970 (Mouse)
Alternate Names
CHC22; Clathrin Heavy Chain 2; clathrin heavy chain on chromosome 22; clathrin, heavy chain-like 1; Clathrin, heavy polypeptide D; CLH-22; CLTCL; CLTCL1; CLTD; heavy polypeptide-like 1

Product Datasheets

Citation for Human Clathrin Heavy Chain 2/CHC22 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Clathrin heavy chain 22 contributes to the control of neuropeptide degradation and secretion during neuronal development
    Authors: MS Nahorski, GHH Borner, SS Shaikh, AK Davies, L Al-Gazali, R Antrobus, CG Woods
    Sci Rep, 2018;8(1):2340.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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