Human Complement Component C1s Antibody

Catalog # Availability Size / Price Qty
MAB2060
MAB2060-SP
Product Details
Citations (1)
FAQs
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Human Complement Component C1s Antibody Summary

Species Reactivity
Human
Specificity
Detects human Complement Component C1s in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human C1r is observed.
Source
Monoclonal Mouse IgG1 Clone # 306904
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Complement Component C1s
Glu16-Asp688
Accession # P09871
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Complement Component C1s (Catalog # 2060-SE) under non-reducing conditions only
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Complement Component C1s (Catalog # 2060-SE), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Complement Component C1s

The classical complement pathway plays a major role in innate immunity against infection. This pathway is triggered by C1, a multimolecular complex composed of the recognition protein C1q and two serine proteases, C1r and C1s. Following the C1q recognition, C1r is autoactivated, and in turn activates C1s, which cleaves C4 and C2, the C1 substrates (1). Both C1r and C1s activation involve cleavage of a specific Arg-Ile bond, converting single-chain proenzymes into active proteases of disulfide bond-linked chains (A and B) (2). The A chains contain multiple domains in the order of CUB1-EGF-CUB2-CCP1-CCP2-Activation Peptide. The B chains contain the serine protease catalytic domain. The full-length (amino acid residues 1‑688) of human C1s was expressed (3‑5). The purified protein corresponded to the processed active form, with A and B chains starting at residue Glu16 and Ile438, respectively.

References
  1. Arlaud, G.J. et al. (2002) Biochem. Soc. Trans. 30:1001.
  2. Lacroix, M. et al. (2001) J. Biol. Chem. 276:36233.
  3. Tosi, M. et al. (1987) Biochemistry 26:8516.
  4. Mackinnon, C.M. et al. (1987) Eur. J. Biochem. 169:547.
  5. Kusumoto, H. et al. (1988) Proc. Natl. Acad. Sci. USA 85:7307.
Entrez Gene IDs
716 (Human)
Alternate Names
basic proline-rich peptide IB-1; C1 esterase; C1S; complement C1s subcomponent; Complement component 1 subcomponent s; complement component 1, s subcomponent; Complement Component C1s; EC 3.4.21; EC 3.4.21.42; FLJ44757

Product Datasheets

Citation for Human Complement Component C1s Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. EDTA/gelatin zymography method to identify C1s versus activated MMP-9 in plasma and immune complexes of patients with systemic lupus erythematosus
    Authors: E Ugarte-Ber, E Martens, L Boon, J Vandooren, D Blockmans, P Proost, G Opdenakker
    J. Cell. Mol. Med., 2019;23(1):576-585.
    Species: Human
    Sample Types: Plasma
    Applications: Zymography

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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