Key Product Details

Species Reactivity

Human

Applications

Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 887025
View all Complement Component C9 Primary Antibodies »

Product Specifications

Immunogen

HEK293 human embryonic kidney cell line transfected with human Complement Component C9
Met1-Lys559
Accession # P02748

Specificity

Detects human Complement Component C9 in ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Complement Component C9 Antibody

Detection of Human Complement Component C9 antibody by Western Blot.

Detection of Human Complement Component C9 by Western Blot.

Western blot shows human plasma. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Complement Component C9 Monoclonal Antibody (Catalog # MAB8126) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Complement Component C9 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human Complement Component C9 antibody by Simple WesternTM.

Detection of Human Complement Component C9 by Simple WesternTM.

Simple Western lane view shows human plasma, loaded at 0.5 mg/mL. A specific band was detected for Complement Component C9 at approximately 78 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Complement Component C9 Monoclonal Antibody (Catalog # MAB8126). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human Complement Component C9 Antibody

Application
Recommended Usage

Simple Western

10 µg/mL
Sample: Human plasma

Western Blot

1 µg/mL
Sample: Human plasma

Reviewed Applications

Read 1 review rated 2 using MAB8126 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Complement Component C9

Complement Component 9 (C9) is a 65-70 kDa protein of the complement membrane attack complex (C5b-9) that binds C5b-8, polymerizes, and forms pores upon activation of the complement cascade. Mature human C9 shares 60% and 66% amino acid sequence identity with mouse and rat C9, respectively. It may be proteolytically cleaved by thrombin into a 28-34 kDa cysteine-rich, mannosylated soluble C9a subunit, and a 37-38 kDa membrane-anchored N-glycosylated C9b. These fragments are also able to polymerize.

Alternate Names

complement component 9, complement component C9

Entrez Gene IDs

735 (Human); 12279 (Mouse); 117512 (Rat)

Gene Symbol

C9

UniProt

P02748

Additional Complement Component C9 Products

Product Documents for Human Complement Component C9 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Complement Component C9 Antibody

For research use only

Citations for Human Complement Component C9 Antibody

Customer Reviews for Human Complement Component C9 Antibody (1)

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  • Human Complement Component C9 Antibody
    Name: Anonymous
    Application: complement membrane attack complex (C5b-9) detection
    Sample Tested: Human serum
    Species: human serum
    Verified Customer | Posted 01/18/2023
    unable to detect MAC formation performed by Zymosan activation in human serum
    Human Complement Component C9 Antibody MAB8126
    Bio-Techne Response
    Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction. The customer worked with our Technical Support team and had success detecting the complexed form of Complement Component C9 in the complement membrane attack complex (C5b-9) while using a polyclonal antibody for Human Complement Component C9 (AF8126). We have not tested complement membrane attack complex (C5b-9) detection with MAB8126 in-house.

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