Detection of Human Complement Component C9 by Western Blot.
Western blot shows human plasma. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Complement Component C9 Monoclonal Antibody (Catalog # MAB8126) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Complement Component C9 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Complement Component C9 by Simple WesternTM.
Simple Western lane view shows human plasma, loaded at 0.5 mg/mL. A specific band was detected for Complement Component C9 at approximately 78 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Complement Component C9 Monoclonal Antibody (Catalog # MAB8126). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Complement Component C9
Complement Component 9 (C9) is a 65-70 kDa protein of the complement membrane attack complex (C5b-9) that binds C5b-8, polymerizes, and forms pores upon activation of the complement cascade. Mature human C9 shares 60% and 66% amino acid sequence identity with mouse and rat C9, respectively. It may be proteolytically cleaved by thrombin into a 28-34 kDa cysteine-rich, mannosylated soluble C9a subunit, and a 37-38 kDa membrane-anchored N-glycosylated C9b. These fragments are also able to polymerize.
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