Human Complement Component C9 Antibody

Catalog # Availability Size / Price Qty
MAB8126
MAB8126-SP
Human Complement Component C9 Antibody in Western Blot
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Human Complement Component C9 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Complement Component C9 in ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 887025
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
HEK293 human embryonic kidney cell line transfected with human Complement Component C9
Met1-Lys559
Accession # P02748
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Complement Component C9 by Western Blot. View Larger

Detection of Human Complement Component C9 by Western Blot. Western blot shows human plasma. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Complement Component C9 Monoclonal Antibody (Catalog # MAB8126) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Complement Component C9 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human Complement Component C9 by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human Complement Component C9 by Simple WesternTM. Simple Western lane view shows human plasma, loaded at 0.5 mg/mL. A specific band was detected for Complement Component C9 at approximately 78 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Complement Component C9 Monoclonal Antibody (Catalog # MAB8126). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Complement Component C9

Complement Component 9 (C9) is a 65-70 kDa protein of the complement membrane attack complex (C5b-9) that binds C5b-8, polymerizes, and forms pores upon activation of the complement cascade. Mature human C9 shares 60% and 66% amino acid sequence identity with mouse and rat C9, respectively. It may be proteolytically cleaved by thrombin into a 28-34 kDa cysteine-rich, mannosylated soluble C9a subunit, and a 37-38 kDa membrane-anchored N-glycosylated C9b. These fragments are also able to polymerize.

Entrez Gene IDs
735 (Human); 12279 (Mouse); 117512 (Rat)
Alternate Names
complement component 9; Complement Component C9

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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