Human Contactin-2/TAG1 Antibody

Catalog #: MAB17141
2 Citations Datasheet / COA / SDS

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MAB17141
MAB17141-SP

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Detection of Human Contactin‑2/TAG1 by Western Blot.

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Citations (2)

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Human Contactin-2/TAG1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Contactin‑2/TAG1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Contactin‑4, rhContactin‑5, recombinant mouse (rm) Contactin‑2, and rmContactin‑3 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 372913

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Contactin‑2/TAG1
Leu29-Asn1012
Accession # Q02246

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Contactin‑2/TAG1 by Western Blot. Western blot shows lysates of human cerebellum tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Contactin-2/TAG1 Monoclonal Antibody (Catalog # MAB17141) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Contactin-2/TAG1 at approximately 135 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Contactin-2/TAG1

Contactin-2 (CNTN2), also called TAG-1 (transient axonal glycoprotein), TAX1 (transiently-expressed axonal glycoprotein), or axonin-1, is a 135 kDa glycosyl‑phosphatidylinositol (GPI)- anchored cell adhesion molecule that belongs to the contactin subfamily within the immunoglobulin (Ig) protein superfamily (1‑3). Human Contactin-2 cDNA encodes a 28 amino acid (aa) signal peptide, a 984 aa mature secreted protein with six Ig-like domains followed by four fibronectin type III‑like repeats, and a 28 aa C-terminal GPI anchor pro-sequence. GPI-specific phospholipase activity can release soluble, active Contactin-2 from the membrane (2). Mature human Contactin-2 shares approximately 93%, 93% and 75% aa sequence identity with human, rat and chicken Contactin-2, respectively. During development, Contactin-2 is expressed by a subset of neuronal populations in the central nervous system (CNS) and peripheral nervous system (PNS), particularly during initial phases of axon outgrowth (3‑5). Both the 135 kDa form and a 90 kDa form are also upregulated in response to CNS injury in the adult (6). Data support a role for Contactin-2 in axon pathfinding, neurite outgrowth and adhesion, especially in the CNS (36). In mature myelinated fibers, Contactin-2 is expressed by oligodendrocytes and Schwann cells, which are myelinating glial cells of the CNS and PNS, respectively (7, 8). It is enriched in the juxtaparanodal regions, where it recruits caspr2 (Contactin-associated protein 2), a transmembrane neurexin involved in cell adhesion and intercellular communication (7‑10). The axonal Contactin-2 interacts in cis with caspr2, and in trans with another Contactin-2 on the glial membrane (8). This ternary complex is required for the accumulation and organization of K⁺channels in the juxtaparanodes (9).

References

Wolfer, D. & R. J. Giger (1994) Swissprot accession # Q61330.
Hasler, T.H. et al. (1993) Eur. J. Biochem. 211:329.
Karagogeos, D. (2003) Front. Biosci.8:s1304.
Liu, Y. & M.C. Halloran (2005) J. Neurosci. 25:10556.
Denaxa, M. et al. (2005) Dev. Biol. 288:87.
Soares, S. et al. (2005) Eur. J. Neurosci. 21:1169.
Traka, M. et al. (2002) J. Neurosci. 22:3016.
Poliak, S. and E. Peles (2003) Nature Reviews Neurosci. 4:968.
Traka, M. et al. (2003) J. Cell Biol. 162:1161.
Poliak, S. et al. (2003) J. Cell Biol. 162:1149.

Entrez Gene IDs

6900 (Human); 21367 (Mouse)

Alternate Names

Axonal glycoprotein TAG-1; axonin-1 cell adhesion molecule; Axonin-1; AXT; CNTN2; contactin 2 (axonal); contactin 2 (transiently expressed); Contactin2; Contactin-2; DKFZp781D102; FLJ37193; MGC157722; TAG1; TAG-1; TAX; TAX1; TAX-1; TAX1FLJ42746; Transient axonal glycoprotein 1; transiently-expressed axonal glycoprotein

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Citations for Human Contactin-2/TAG1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Molecular Markers in Maternal Blood Exosomes Allow Early Detection of Fetal Alcohol Spectrum Disorders
Authors: N Darbinian, A Darbinyan, J Sinard, G Tatevosian, N Merabova, F D'Amico, T Khader, A Bajwa, D Martirosya, AK Gawlinski, R Pursnani, H Zhao, S Amini, M Morrison, L Goetzl, ME Selzer
International Journal of Molecular Sciences, 2022-12-21;24(1):.
Species: Human
Sample Types: Exosomes
Applications: Flow Cytometry
Intrathecal CD4(+) and CD8(+) T-cell responses to endogenously synthesized candidate disease-associated human autoantigens in multiple sclerosis patients.
Authors: van Nierop G, Janssen M, Mitterreiter J, van de Vijver D, de Swart R, Haagmans B, Verjans G, Hintzen R
Eur J Immunol, 2015-11-09;46(2):347-53.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry