Human Contactin-2/TAG1 Antibody

  (1 citations)     
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  • Species Reactivity
    Human
  • Specificity
    Detects human Contactin‑2/TAG1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Contactin‑4, rhContactin‑5, recombinant mouse (rm) Contactin‑2, and rmContactin‑3 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 372913
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Contactin‑2/TAG1
    Leu29-Asn1012
    Accession # Q02246
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human Contactin‑2/TAG1 (Catalog # 1714-CN)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Contactin-2/TAG1

Contactin-2 (CNTN2), also called TAG-1 (transient axonal glycoprotein), TAX1 (transiently-expressed axonal glycoprotein), or axonin-1, is a 135 kDa glycosyl‑phosphatidylinositol (GPI)- anchored cell adhesion molecule that belongs to the contactin subfamily within the immunoglobulin (Ig) protein superfamily (1‑3). Human Contactin-2 cDNA encodes a 28 amino acid (aa) signal peptide, a 984 aa mature secreted protein with six Ig-like domains followed by four fibronectin type III‑like repeats, and a 28 aa C-terminal GPI anchor pro-sequence. GPI-specific phospholipase activity can release soluble, active Contactin-2 from the membrane (2). Mature human Contactin-2 shares approximately 93%, 93% and 75% aa sequence identity with human, rat and chicken Contactin-2, respectively. During development, Contactin-2 is expressed by a subset of neuronal populations in the central nervous system (CNS) and peripheral nervous system (PNS), particularly during initial phases of axon outgrowth (3‑5). Both the 135 kDa form and a 90 kDa form are also upregulated in response to CNS injury in the adult (6). Data support a role for Contactin-2 in axon pathfinding, neurite outgrowth and adhesion, especially in the CNS (36). In mature myelinated fibers, Contactin-2 is expressed by oligodendrocytes and Schwann cells, which are myelinating glial cells of the CNS and PNS, respectively (7, 8). It is enriched in the juxtaparanodal regions, where it recruits caspr2 (Contactin-associated protein 2), a transmembrane neurexin involved in cell adhesion and intercellular communication (7‑10). The axonal Contactin-2 interacts in cis with caspr2, and in trans with another Contactin-2 on the glial membrane (8). This ternary complex is required for the accumulation and organization of K+ channels in the juxtaparanodes (9).

  • References:
    1. Wolfer, D. & R. J. Giger (1994) Swissprot accession # Q61330.
    2. Hasler, T.H. et al. (1993) Eur. J. Biochem. 211:329.
    3. Karagogeos, D. (2003) Front. Biosci.8:s1304.
    4. Liu, Y. & M.C. Halloran (2005) J. Neurosci. 25:10556.
    5. Denaxa, M. et al. (2005) Dev. Biol. 288:87.
    6. Soares, S. et al. (2005) Eur. J. Neurosci. 21:1169.
    7. Traka, M. et al. (2002) J. Neurosci. 22:3016.
    8. Poliak, S. and E. Peles (2003) Nature Reviews Neurosci. 4:968.
    9. Traka, M. et al. (2003) J. Cell Biol. 162:1161.
    10. Poliak, S. et al. (2003) J. Cell Biol. 162:1149.
  • Entrez Gene IDs:
    6900 (Human)
  • Alternate Names:
    Axonal glycoprotein TAG-1; axonin-1 cell adhesion molecule; Axonin-1; AXT; CNTN2; contactin 2 (axonal); contactin 2 (transiently expressed); Contactin2; Contactin-2; DKFZp781D102; FLJ37193; MGC157722; TAG1; TAG-1; TAX; TAX1; TAX-1; TAX1FLJ42746; Transient axonal glycoprotein 1; transiently-expressed axonal glycoprotein
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Intrathecal CD4(+) and CD8(+) T-cell responses to endogenously synthesized candidate disease-associated human autoantigens in multiple sclerosis patients.
    Authors: van Nierop G, Janssen M, Mitterreiter J, van de Vijver D, de Swart R, Haagmans B, Verjans G, Hintzen R
    Eur J Immunol, 2015;46(2):347-53.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow

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Isotype Controls
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Mouse IgG1 Isotype Control

Ctrl MAB002 147  
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Mouse IgG1 Isotype Control

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Reconstitution Buffers
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Reconstitution Buffer 1 (PBS)

RB01
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Secondary Antibodies
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Flow , IHC , ICC NL007 10
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow , IHC , ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 3  
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG Antibody

WB AF007 4
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow , IHC , ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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