Human Contactin-2/TAG1 DuoSet ELISA

R&D Systems | Catalog # DY1714-05

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Key Product Details

Assay Type

Solid Phase Sandwich ELISA

Assay Range

156-10000 pg/mL

Sample Type

Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet

Reactivity

Human

Human Contactin-2/TAG1 DuoSet ELISA Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits
View all Contactin-2/TAG1 ELISA Kits »

Product Summary for Human Contactin-2/TAG1 DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Contactin-2 / TAG1. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Specifications

Assay Format

96-well strip plate (sold separately)

Sample Volume Required

100 µL

Detection Method

Colorimetric ELISA - 450nm (TMB)

Conjugate

Biotin

Specificity

Please see the product datasheet

Label

HRP

Scientific Data Images for Human Contactin-2/TAG1 DuoSet ELISA

Human Contactin-2 / TAG1 ELISA Standard Curve

Human Contactin-2 / TAG1 ELISA Standard Curve

Kit Contents for Human Contactin-2/TAG1 DuoSet ELISA

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008C) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: ELISA TMB Substrate (Catalog # DY999B or DY999B-250)

Stop Solution: Methanesulfonic acid (Catalog # DY994B or DY994B-250)

Microplates: (Catalog # DY990), or equivalent

Plate Sealers: (Catalog # DY992), or equivalent

*For the recommended Reagent Diluent and Blocking Buffer for a specific DuoSet ELISA Development Kit, refer to the product datasheet.

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Contactin-2/TAG1

Contactin-2 (CNTN2), also known as TAX1 (human), TAG1 (rat), and Axonin-1 (chicken), is a cell adhesion molecule that belongs to the contactin subfamily within the immunoglobulin (Ig) protein superfamily. Contactin-2 contains 6 Ig-like domains followed by 4 fibronectin type III-like repeats. The gene for human CNTN2 encodes a 1040 aa precursor protein with a 28 aa signal peptide and a 984 aa mature secreted protein that is attached to the membrane via a glycosyl-phosphatidylinositol(GPI)-anchor.

Alternate Names

Axonin-1, AXT, CNTN2, Contactin2, TAG1, TAX1

Entrez Gene IDs

6900 (Human); 21367 (Mouse)

Gene Symbol

CNTN2

Additional Contactin-2/TAG1 Products

Product Documents for Human Contactin-2/TAG1 DuoSet ELISA

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Contactin-2/TAG1 DuoSet ELISA

For research use only

Related Research Areas

Immunoglobulin Superfamily CAMs

Citations for Human Contactin-2/TAG1 DuoSet ELISA

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