Detection of Human CTGF/CCN2 by Western Blot.
Western blot shows lysates of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CTGF/CCN2 Monoclonal Antibody (Catalog # MAB9190) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CTGF/CCN2 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
CTGF belongs to the CCN (CYR61/CTGF/NOV) family of secreted proteins
that share a common multimodular organization. Each protein contains
IGF-binding protein domain, a von Willebrand factor type C domain, a
thrombospondin type I domain, and a cysteine knot domain. The
multimodular CTGF has the ability to bind multiple ligands and has
numerous effects on development, differentation, and disease. The
C-terminal cysteine knot motif contains the heparin and low density
lipoprotein receptor (LDLR) binding sites that contributes to the
adhesive and mitogenic properties of CTGF.
Connective Tissue Growth Factor
Entrez Gene IDs:
1490 (Human); 14219 (Mouse); 64032 (Rat)
CCN2; CCN2IGFBP-8; connective tissue growth factor; CTGF; CTGRP; Fisp12; HCS24; Hypertrophic chondrocyte-specific protein 24; IBP-8; IGF-binding protein 8; IGFBP-8; IGFBP8CCN family member 2; Insulin-like growth factor-binding protein 8; MGC102839; NOV2
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