Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Neutralization, Flow Cytometry, CyTOF-ready

Cited:

Neutralization

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # 81506R
View all CX3CL1/Fractalkine Primary Antibodies »

Product Specifications

Immunogen

NS0-derived and E. coli-derived Recombinant human CX3CL1/Fracktalkine
Gln25-Arg339
Accession # P78423

Specificity

Detects human CX3CL1/Fractalkine in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human CX3CL1/Fractalkine Antibody

Detection of CX3CL1/Fractalkine in HEK293 cells transfected with hCX3CL1 vs Irrelevant HEK293 transfectant cells by Flow Cytometry

HEK293 cells transfected with hCX3CL1 (A) vs Irrelevant HEK293 transfectant cells (B) were stained with Mouse Anti-Human CX3CL1/Fractalkine Chemokine Domain Monoclonal Antibody (Catalog # MAB3652R) and eGFP isotype control antibody (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
Chemotaxis Induced by CX3CL1/Fractalkine and Neutralization by Human CX3CL1/Fractalkine Antibody.

Chemotaxis Induced by CX3CL1/Fractalkine and Neutralization by Human CX3CL1/Fractalkine Antibody.

Recombinant Human CX3CL1/Fractalkine (Catalog # 365-FR) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CX3CR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CX3CL1/Fractalkine (100 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CX3CL1/Fractalkine Chemokine Domain Monoclonal Antibody (Catalog # MAB3652). The ND50 is typically 1-5 µg/mL.

Applications for Human CX3CL1/Fractalkine Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 cell Line transfected with Human CX3CL1/Fractalkine

Neutralization

Measured by its ability to neutralize CX3CL1/Fractalkine-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CX3CR1. The Neutralization Dose (ND50) is typically 1-5 µg/mL in the presence of 100 ng/mL Recombinant Human CX3CL1/Fractalkine.

Flow Cytometry Panel Builder

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CX3CL1/Fractalkine

CX3CL1, also known as Fractalkine, is a type I membrane protein in which a chemokine domain possessing a unique C-X3-C cysteine motif is tethered on a long mucin-like stalk. It can also be released as a soluble molecule upon proteolysis at a membrane proximal site.

Alternate Names

FKN, Fractalkine, Neurotactin

Entrez Gene IDs

6376 (Human); 20312 (Mouse); 89808 (Rat); 102117496 (Cynomolgus Monkey)

Gene Symbol

CX3CL1

UniProt

P78423

Additional CX3CL1/Fractalkine Products

Product Documents for Human CX3CL1/Fractalkine Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human CX3CL1/Fractalkine Antibody

For research use only

Citations for Human CX3CL1/Fractalkine Antibody

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