Human/Cynomolgus Monkey FCAR/CD89 Antibody

Catalog # Availability Size / Price Qty
MAB3939
MAB3939-SP
Detection of FCAR/CD89 in Human Granulocytes by Flow Cytometry.
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Human/Cynomolgus Monkey FCAR/CD89 Antibody Summary

Species Reactivity
Human, Cynomolgus Monkey
Specificity
Detects human FCAR/CD89 in direct ELISAs. Detects human and cynomolgus monkey FCAR/CD89 in Flow cytometry. In direct ELISAs, no cross-reactivity with Fc gamma RIA, RIIA, or RIIIB is observed.
Source
Monoclonal Mouse IgG1 Clone # 488032
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human FCAR/CD89
Gln22-Lys287
Accession # P24071
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of FCAR/CD89 antibody in Human Granulocytes antibody by Flow Cytometry. View Larger

Detection of FCAR/CD89 in Human Granulocytes by Flow Cytometry. Human blood-derived granulocytes were stained with Mouse Anti-Human FCAR/CD89 Monoclonal Antibody (Catalog # MAB3939, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B).

Flow Cytometry Detection of FCAR/CD89 antibody in HEK293 Human Cell Line Transfected with Cynomolgus FCAR/CD89 and eGFP antibody by Flow Cytometry. View Larger

Detection of FCAR/CD89 in HEK293 Human Cell Line Transfected with Cynomolgus FCAR/CD89 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with Cynomolgus FCAR/CD89 and eGFP was stained with (A) Mouse Anti-Human FCAR/CD89 Monoclonal Antibody (Catalog # MAB3939) or (B) Mouse IgG1 isotype control antibody (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FCAR/CD89

FCAR, also called Fc alpha RI or CD89, is a variably glycosylated 50‑100 kDa myeloid-specific type I transmembrane (TM) Fc receptor for IgA that is a member of the multichain immune recognition receptor (MIRR) family (1‑3). Human FCAR contains a 21 amino acid (aa) signal sequence and extracellular (ECD), TM and cytoplasmic domains of 206, 19 and 41 aa, respectively (4). Arg230 within the TM domain supports interaction with the ITAM-containing signaling subunit, FcR gamma, which contains a TM Asp (5‑7). Two ECD C2-type Ig-like domains (EC1 and 2) are oriented at right angles (8). Up to two molecules of FCAR can bind one molecule of serum IgA via EC1 (8). Many splice variants have been reported, but only two have been identified as proteins (9, 10). The a.2 form, which lacks 22 aa just prior to the TM domain, is exclusively expressed in alveolar macrophages. The a.3 form lacks EC2. FCAR binds monomeric, polymeric and secretory IgA, but does not mediate the barrier function of secretory IgA in mucosal epithelium (1‑3). Shedding and circulation of polymeric IgA/FCAR immune complexes has been reported (11). Circulating neutrophils, eosinophils, and monocytes express FCAR (12). Tissue expression of FCAR is mainly from neutrophils; FCAR is downregulated as monocytes differentiate to tissue macrophages (12). On neutrophils, a significant amount of FCAR lacks FcR gamma, but can still be endocytosed to early endosomes and recycled to the cell surface (5). Binding of serum IgA to FCAR is transient and anti-inflammatory, inhibiting IgG or IgE-induced degranulation (6). Sustained aggregation of FCAR results in inflammatory responses (6). FcR gamma signaling is required for these and for transport to late endosomes (5‑7). Human FCAR shows 55‑58% aa identity with rat, horse and cow FCAR. No ortholog occurs in mouse. FCAR structure resembles the KIR/ILT/LIR/MIR family more than other IgA receptors, including pIgR, Fc alpha /μR, asialoglycoprotein receptor (ASGR1) and transferrin receptor (TfR) (1‑3).

References
  1. Wines, B. D. and P. M. Hogarth (2006) Tissue Antigens 68:103.
  2. Otten, M. A. and M. van Egmon (2004) Immunol. Lett. 92:23.
  3. Montiero, R. C. and J. G. J. van de Winkel (2003) Annu. Rev. Immunol. 21:177.
  4. Maliszewski, C. R. et al. (1990) J. Exp. Med. 172:1665.
  5. Launay, P. et al. (1999) J. Biol. Chem. 274:7216.
  6. Pasquier, B. et al. (2005) Immunity 22:31.
  7. Shen, L. et al. (2001) Blood 97:205.
  8. Herr, Y. et al. (2003) Nature 423:614.
  9. Patry, C. et al. (1996) J. Immunol. 156:4442.
  10. Togo, S. et al. (2003) FEBS Lett. 535:205.
  11. van der Boog, P. J. M. et al. (2002) J. Immunol. 168:1252.
  12. Hamre, R. et al. (2003) Scand. J. Immunol. 57:506.
Long Name
IgA Fc Receptor
Entrez Gene IDs
2204 (Human); 365183 (Rat); 102145896 (Cynomolgus Monkey)
Alternate Names
CD89 antigen; CD89; CD89IgA Fc receptor; Fc alpha receptor; Fc fragment of IgA, receptor for; FCAR; immunoglobulin alpha Fc receptor

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