Human Desmoplakin Antibody

Catalog # Availability Size / Price Qty
MAB9080
MAB9080-SP
Detection  of Human Desmoplakin by Western Blot.
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Human Desmoplakin Antibody Summary

Species Reactivity
Human
Specificity
Detects human Desmoplakin in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 824038
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Desmoplakin
Asn1796-Val2046
Accession # P15924
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection  of Human Desmoplakin by Western Blot. View Larger

Detection of Human Desmoplakin by Western Blot. Western blot shows lysate of COLO 205 human colorectal adenocarcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Desmoplakin Monoclonal Antibody (Catalog # MAB9080) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Desmoplakin at approximately 260 and 330 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Desmoplakin

DSP (desmoplakin) is the major high molecular weight protein of desmosomes, the most common type of intercellular junction in vertebrate epithelial cells.  DSP is involved in the organization of the desmosomal cadherin-plakoglobin complex, as well as in the anchoring of intermediate filaments to desmosomes.  These functions are thought to impart tensile strength and integrity to epithelial and vascular tissues.  Mutations in DSP result in several clinical phenotypes, ranging from mild skin conditions, to severe skin blistering with subsequent dehydration, to lethal heart defects.  Two major desmoplakin isoforms, DSPI and DSPII, are encoded by alternative mRNA transcripts differentially spliced from the same gene.  Human DSPI is 2,871 amino acids (aa) in length, while DSPII lacks part of a
central alpha-helical rod domain, and is 599 aa shorter.

Entrez Gene IDs
1832 (Human); 109620 (Mouse); 306871 (Rat)
Alternate Names
250/210 kDa paraneoplastic pemphigus antigen; DCWHKTA; desmoplakin (DPI, DPII); desmoplakin I; desmoplakin II; Desmoplakin; DP; DPI; DPII; DSP; KPPS2; PPKS2

Product Datasheets

FAQs

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Isotype Controls

Mouse IgG1 Isotype Control

Ctrl

Reconstitution Buffers

Secondary Antibodies

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