Detection of Human Desmoplakin by Western Blot.
Western blot shows lysate of COLO 205 human colorectal adenocarcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Desmoplakin Monoclonal Antibody (Catalog # MAB9080) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Desmoplakin at approximately 260 and 330 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
(desmoplakin) is the major high molecular weight protein of desmosomes, the
most common type of intercellular junction in vertebrate epithelial cells. DSP is involved in the organization of the
desmosomal cadherin-plakoglobin complex, as well as in the anchoring of
intermediate filaments to desmosomes.
These functions are thought to impart tensile strength and integrity to
epithelial and vascular tissues.
Mutations in DSP result in several clinical phenotypes, ranging from
mild skin conditions, to severe skin blistering with subsequent dehydration, to
lethal heart defects. Two major
desmoplakin isoforms, DSPI and DSPII, are encoded by alternative mRNA
transcripts differentially spliced from the same gene. Human DSPI is 2,871 amino acids (aa) in length,
while DSPII lacks part of a central alpha-helical rod domain, and is 599 aa
Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.