Human Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody

Catalog # Availability Size / Price Qty
AF6530
AF6530-SP
Human Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody in Immunohistochemistry
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Human Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Dimethylarginine Dimethylaminohydrolase 1/DDAH1 in direct ELISAs.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Dimethylarginine Dimethylaminohydrolase 1/DDAH1
Ala2-Ser285
Accession # O94760
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry Dimethylarginine Dimethylaminohydrolase 1/DDAH1 in Human Brain. View Larger

Dimethylarginine Dimethylaminohydrolase 1/DDAH1 in Human Brain.

Dimethylarginine Dimethylaminohydrolase 1/
DDAH1 was detected in immersion fixed paraffin-embedded sections of human brain using Sheep Anti-Human Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6530) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counter­stained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Dimethylarginine Dimethylaminohydrolase 1/DDAH1

Dimethylarginine Dimethylaminohydrolase (DDAH) metabolizes asymmetric dimethyl arginine (ADMA) to L-citrulline and dimethylamine, and NG-monomethyl arginine (MMA) to L-citrulline and monomethylamine (1). Two members of the DDAH family have been identified in humans. DDAH1 is widely expressed, especially in liver and kidney. DDAH2 predominates in vascular endothelium and expressed selectively in kidney (2). It is also expressed in immune tissues including spleen, thymus, peripheral leukocytes, lymph nodes, and bone marrow. Over 90 % of endogenous ADMA is metabolized by DDAH with the remainder excreted (3). ADMA and MMA are endogenous inhibitors of nitric oxide synthase (NOS). Thus, enzymes of the DDAH family play a key role in vascular function through the turnover of methylated arginine (4). It has been observed that genetic variation in the DDAH1 and DDAH2 genes is significantly associated with serum ADMA levels (5).

References
  1. Tadashi, O. et al. (1989) J. Biol. Chem. 264:10205.
  2. Tran, C. T. et al. (2000) Genomics 68:101.
  3. Tran, C. T. et al. (2003) Atheroscler. Suppl. 4:33.
  4. Leiper, J. et al. (2007) Nat. Med. 13:198.
  5. Abhary, S. et al. (2010) PNAS PLoS One. 5:e9462.
Entrez Gene IDs
23576 (Human)
Alternate Names
DDAH1; DDAH-1; DDAHdimethylargininase-1; DDAHI; Dimethylargininase-1; dimethylarginine dimethylaminohydrolase 1FLJ25539; EC 3.5.3.18; FLJ21264; N(G); N(G)-dimethylarginine dimethylaminohydrolase 1; NG, NG-dimethylarginine dimethylaminohydrolase

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