Detects human Dishevelled-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human Dishevelled-1 and recombinant human Dishevelled-2 is observed.
Polyclonal Sheep IgG
E. coli-derived recombinant human Dishevelled-3 His545-Gly654 Accession # Q92997
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Immersion fixed paraffin-embedded sections of human liver cancer tissue
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human and Mouse Dishevelled-3 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, A549 human lung carcinoma cell line, and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Dishevelled‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3754) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Dishevelled-3 at approximately XXX kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group XXX.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Members of the Dishevelled family are cytoplasmic phosphoproteins, and orthologs of the Drosophila dishevelled segment polarity protein. Dishevelleds are expressed in multiple tissues where they are well known for their participation in Wnt signal transduction. They are modular proteins that connect Wnt ligand engagement with Frizzled receptors, to proteins involved in the stabilization of beta-catenin in the canonical Wnt pathway. Upon Wnt binding, Dishevelled becomes associated with the beta-catenin complex and blocks GSK-3 beta kinase activity. This leads to beta-catenin accumulation, the activation of LEF/TCF transcription factors, and transcription of their target genes.
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