Human DSPG3 Antibody

Catalog # Availability Size / Price Qty
MAB3110
MAB3110-SP
DSPG3 in Human HUVEC.
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Human DSPG3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human DSPG3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse DSPG3 is observed.
Source
Monoclonal Mouse IgG1 Clone # 329707
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human DSPG3
Ala20-Val322
Accession # Q99645
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human DSPG3 (Catalog # 3110-DS)
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry DSPG3 antibody in Human HUVEC by Immunocytochemistry (ICC). View Larger

DSPG3 in Human HUVEC. DSPG3 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Mouse Anti-Human DSPG3 Monoclonal Antibody (Catalog # MAB3110) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DSPG3

Human DSPG3 (also PG-Lb and epiphycan) is a 95‑100 kDa class III subfamily member of the SLRP family of molecules. It is synthesized as a 322 aa precursor that contains a 19 aa signal sequence and a 303 aa mature region. The mature molecule contains two intrachain disulfide bonds that flank six leucine-rich repeats lying between aa 135-301. There is a potential for both O- and N-linked glycosylation. DSPG3 is found principally in cartilage. Mature human DSPG3 is 85%, 96%, 95% and 84% aa identical to rat, dog, cow and mouse DSPG3, respectively.

Long Name
Dermatan Sulfate Proteoglycan 3
Entrez Gene IDs
1833 (Human); 13516 (Mouse)
Alternate Names
Dermatan sulfate proteoglycan 3dermatan sulphate proteoglycan 3; DSPG3; DSPG3proteoglycan-lb; Epiphycan; EPYC; PGLB; PG-Lb; Proteoglycan-Lb; SLRR3B; SLRR3Bepiphycan proteoglycan; Small chondroitin/dermatan sulfate proteoglycan

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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