Human DUX4/DUX4c Antibody Summary
Accession # Q9UBX2
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human DUX4/DUX4c by Western Blot. Western blot shows lysates of C2C12 mouse myoblast cell line transfected with human DUX4 untreated (-) or treated (+) with 100 ng/mL Doxycycline for overnight. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human DUX4 Monoclonal Antibody (Catalog # MAB95351) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for DUX4 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Human Double homeobox 4 (aka DUX4) is a protein encoded by the DUX4 gene. This gene is located within a D4Z4 repeat array in chromosome 4q35. Each D4Z4 repeat unit has an open reading frame, DUX4, that contains two homeoboxes. Unregulated expression of DUX4 in muscle cells is the cause of facioscapulohumeral muscular dystrophy (FSHD), a common form of muscular dystrophy in adults that is one of the most prevalent genetic diseases of muscle.
Citations for Human DUX4/DUX4c Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
Filter your results:
Transcriptional and cytopathological hallmarks of FSHD in chronic DUX4-expressing mice
Authors: D Bosnakovsk, AS Shams, C Yuan, MT da Silva, ET Ener, CW Baumann, AJ Lindsay, M Verma, A Asakura, DA Lowe, M Kyba
J. Clin. Invest., 2020;0(0):.
Sample Types: Whole Tissue
Crystal Structure of the Double Homeodomain of DUX4 in Complex with DNA
Authors: JK Lee, D Bosnakovsk, EA Toso, T Dinh, S Banerjee, TE Bohl, K Shi, K Orellana, M Kyba, H Aihara
Cell Rep, 2018;25(11):2955-2962.e3.
Sample Types: Cell Lysates
Applications: Western Blot
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Human DUX4/DUX4c Antibody
There are currently no reviews for this product. Be the first to review Human DUX4/DUX4c Antibody and earn rewards!
Have you used Human DUX4/DUX4c Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image