Human DUX4/DUX4c Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB95351-100
MAB95351-SP
Detection of Human DUX4/DUX4c by Western Blot.
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Product Details
Citations (2)
FAQs
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Human DUX4/DUX4c Antibody Summary

Species Reactivity
Human
Specificity
Detects human DUX4/DUX4c in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2143C
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Human DUX4 synthetic peptide containing sequences within amino acids 200-300 of the human DUX4 protein
Accession # Q9UBX2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human DUX4/DUX4c antibody by Western Blot. View Larger

Detection of Human DUX4/DUX4c by Western Blot. Western blot shows lysates of C2C12 mouse myoblast cell line transfected with human DUX4 untreated (-) or treated (+) with 100 ng/mL Doxycycline for overnight. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human DUX4 Monoclonal Antibody (Catalog # MAB95351) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for DUX4 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DUX4/DUX4c

Human Double homeobox 4 (aka DUX4) is a protein encoded by the DUX4 gene. This gene is located within a D4Z4 repeat array in chromosome 4q35. Each D4Z4 repeat unit has an open reading frame, DUX4, that contains two homeoboxes. Unregulated expression of DUX4 in muscle cells is the cause of facioscapulohumeral muscular dystrophy (FSHD), a common form of muscular dystrophy in adults that is one of the most prevalent genetic diseases of muscle.

Long Name
Double Homeobox 4/Double Homeobox 4c
Entrez Gene IDs
22947 (Human)
Alternate Names
DUX4/DUX4c

Product Datasheets

Citations for Human DUX4/DUX4c Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Transcriptional and cytopathological hallmarks of FSHD in chronic DUX4-expressing mice
    Authors: D Bosnakovsk, AS Shams, C Yuan, MT da Silva, ET Ener, CW Baumann, AJ Lindsay, M Verma, A Asakura, DA Lowe, M Kyba
    J. Clin. Invest., 2020;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Crystal Structure of the Double Homeodomain of DUX4 in Complex with DNA
    Authors: JK Lee, D Bosnakovsk, EA Toso, T Dinh, S Banerjee, TE Bohl, K Shi, K Orellana, M Kyba, H Aihara
    Cell Rep, 2018;25(11):2955-2962.e3.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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