Human DUX4/DUX4c Antibody (2143C)

R&D Systems | Catalog # MAB95351

Recombinant Monoclonal Antibody.
R&D Systems

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2143C
View all DUX4/DUX4c Primary Antibodies »

Product Specifications

Immunogen

Human DUX4 synthetic peptide containing sequences within amino acids 200-300 of the human DUX4 protein
Accession # Q9UBX2

Specificity

Detects human DUX4/DUX4c in direct ELISAs.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human DUX4/DUX4c Antibody (2143C)

Detection of Human DUX4/DUX4c antibody by Western Blot.

Detection of Human DUX4/DUX4c by Western Blot.

Western blot shows lysates of C2C12 mouse myoblast cell line transfected with human DUX4 untreated (-) or treated (+) with 100 ng/mL Doxycycline for overnight. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human DUX4 Monoclonal Antibody (Catalog # MAB95351) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for DUX4 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human DUX4/DUX4c Antibody (2143C)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: C2C12 mouse myoblast cell line transfected with human DUX4 treated with Doxycycline

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DUX4/DUX4c

Human Double homeobox 4 (aka DUX4) is a protein encoded by the DUX4 gene. This gene is located within a D4Z4 repeat array in chromosome 4q35. Each D4Z4 repeat unit has an open reading frame, DUX4, that contains two homeoboxes. Unregulated expression of DUX4 in muscle cells is the cause of facioscapulohumeral muscular dystrophy (FSHD), a common form of muscular dystrophy in adults that is one of the most prevalent genetic diseases of muscle.

Long Name

Double Homeobox 4/Double Homeobox 4c

Alternate Names

DUX10, DUX4, DUX4L

Entrez Gene IDs

22947 (Human)

Gene Symbol

DUX4L1

UniProt

Q9UBX2

Additional DUX4/DUX4c Products

Product Documents for Human DUX4/DUX4c Antibody (2143C)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human DUX4/DUX4c Antibody (2143C)

For research use only

Citations for Human DUX4/DUX4c Antibody (2143C)

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