Human DUX4/DUX4c Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
Detection of Human DUX4/DUX4c by Western Blot.
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Product Details
Citations (5)
Supplemental Products

Human DUX4/DUX4c Antibody Summary

Species Reactivity
Detects human DUX4/DUX4c in direct ELISAs.
Recombinant Monoclonal Rabbit IgG Clone # 2143C
Protein A or G purified from cell culture supernatant
Human DUX4 synthetic peptide containing sequences within amino acids 200-300 of the human DUX4 protein
Accession # Q9UBX2
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human DUX4/DUX4c antibody by Western Blot. View Larger

Detection of Human DUX4/DUX4c by Western Blot. Western blot shows lysates of C2C12 mouse myoblast cell line transfected with human DUX4 untreated (-) or treated (+) with 100 ng/mL Doxycycline for overnight. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human DUX4 Monoclonal Antibody (Catalog # MAB95351) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for DUX4 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DUX4/DUX4c

Human Double homeobox 4 (aka DUX4) is a protein encoded by the DUX4 gene. This gene is located within a D4Z4 repeat array in chromosome 4q35. Each D4Z4 repeat unit has an open reading frame, DUX4, that contains two homeoboxes. Unregulated expression of DUX4 in muscle cells is the cause of facioscapulohumeral muscular dystrophy (FSHD), a common form of muscular dystrophy in adults that is one of the most prevalent genetic diseases of muscle.

Long Name
Double Homeobox 4/Double Homeobox 4c
Entrez Gene IDs
22947 (Human)
Alternate Names

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Citations for Human DUX4/DUX4c Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. DUX4-r exerts a neomorphic activity that depends on GTF2I in acute lymphoblastic leukemia
    Authors: Campolungo, D;Salomé, M;Biferali, B;Tascini, AS;Gabellini, D;
    Science advances
    Species: Human
    Sample Types: Protein Extracts
    Applications: Western Blot
  2. Antiapoptotic Protein FAIM2 is targeted by miR-3202, and DUX4 via TRIM21, leading to cell death and defective myogenesis
    Authors: HAN Soliman, EA Toso, IE Darwish, SM Ali, M Kyba
    Cell Death & Disease, 2022-04-25;13(4):405.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Persistent Fibroadipogenic Progenitor Expansion Following Transient DUX4 Expression Provokes a Profibrotic State in a Mouse Model for FSHD
    Authors: D Bosnakovsk, D Oyler, A Mitanoska, M Douglas, ET Ener, AS Shams, M Kyba
    International Journal of Molecular Sciences, 2022-02-11;23(4):.
    Species: Mouse
    Sample Types: Whole Tissues
    Applications: IHC
  4. Transcriptional and cytopathological hallmarks of FSHD in chronic DUX4-expressing mice
    Authors: D Bosnakovsk, AS Shams, C Yuan, MT da Silva, ET Ener, CW Baumann, AJ Lindsay, M Verma, A Asakura, DA Lowe, M Kyba
    J. Clin. Invest., 2020-05-01;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Crystal Structure of the Double Homeodomain of DUX4 in Complex with DNA
    Authors: JK Lee, D Bosnakovsk, EA Toso, T Dinh, S Banerjee, TE Bohl, K Shi, K Orellana, M Kyba, H Aihara
    Cell Rep, 2018-12-11;25(11):2955-2962.e3.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot


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