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Human EMMPRIN/CD147 DuoSet ELISA, 15 Plate

  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Sample Type & Volumne Required
    100 µL
  • Range
    62.50 - 4,000 pg/mL
  • Sufficient Materials
    For fifteen 96-well plates*
  • Specificity
    Please see the product datasheet
  • * Following cell simulation.
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
Product Features
  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits
Kit Content
  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Regeagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Background EMMPRIN/CD147
  • Molecule Information:
    EMMPRIN/CD147
  • Long Name:
    Extracellular Matrix Metalloproteinase Inducer
  • Aliases:
    5F7; basigin; basigin (Ok blood group); CD147; CD147 antigen; Collagenase stimulatory factor; EMMPRINTCSF; Extracellular matrix metalloproteinase inducer; Leukocyte activation antigen M6; M6; OK; OK blood group antigen; Tumor cell-derived collagenase stimulatory factor
  • Entrez Gene IDs:
    682 (Human); 12215 (Mouse);
  • Background:
    EMMPRIN/CD147
The name EMMPRIN reflects this proteins extracellular metalloproteinase inducer activity. It is a cell surface protein with two extracellular Ig domains, a transmembrane domain, and a cytoplasmic domain. Its expression is often elevated in human tumor cells, which in turn promotes MMP-1, MMP-2 and MMP-3 production in melanoma cells and MMP-2 and MMP-9 production in breast cancer cells. It interacts with several types of molecules such as integrins, caveolin-1 and MCT1. TRA-1-85 is an epitope within EMMPRIN.

FAQs

No product specific FAQs exist for this product, however you may click here to view all FAQs for ELISAs

Order Details

  • Catalog #
    DY972

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