Human EMMPRIN/CD147 DuoSet ELISA

  
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Sample Type & Volume Required
    100 µL
  • Range
    62.50 - 4,000 pg/mL
  • Sufficient Materials
    For fifteen 96-well plates*
  • Specificity
    Please see the product datasheet
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
Ancillary Reagent Kit Available

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and Recombinant . The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.


Product Features
  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits
Kit Content
  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: EMMPRIN/CD147
The name EMMPRIN reflects this proteins extracellular metalloproteinase inducer activity. It is a cell surface protein with two extracellular Ig domains, a transmembrane domain, and a cytoplasmic domain. Its expression is often elevated in human tumor cells, which in turn promotes MMP-1, MMP-2 and MMP-3 production in melanoma cells and MMP-2 and MMP-9 production in breast cancer cells. It interacts with several types of molecules such as integrins, caveolin-1 and MCT1. TRA-1-85 is an epitope within EMMPRIN.
  • Entrez Gene IDs:
    682 (Human); 12215 (Mouse);
  • Long Name:
    Extracellular Matrix Metalloproteinase Inducer
  • Aliases:
    5F7; basigin (Ok blood group); Basigin; BSG; CD147 antigen; CD147; Collagenase stimulatory factor; EMMPRINTCSF; Extracellular matrix metalloproteinase inducer; Leukocyte activation antigen M6; M6; OK blood group antigen; OK; Tumor cell-derived collagenase stimulatory factor
DuoSet Ancillary Reagent Kits
Description Application Cat# Citations Images  

DuoSet ELISA Ancillary Reagent Kit 2

ELISA DY008
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Supplemental ELISA Products
Description Application Cat# Citations Images  

Substrate Reagent Pack (8 vials Color A, 8 vials Color B)

ELISA DY999 38
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Reagent Diluent Concentrate 2 (10X, 5 x 21 mL)

ELISA DY995 12
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Stop Solution 2N Sulfuric Acid (15 x 6 mL)

ELISA DY994 7
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ELISA Plate Sealers, 100 Pack

ELISA DY992
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Quantikine Wash Buffer 1

ELISA WA126 5
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Streptavidin-HRP (set of 5 vials enough for 75 plates total)

ELISA DY998 29
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Clear Polystyrene Microplates, 25 Pack

ELISA DY990 2
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Reagent Additive 1 (21 mL)

ELISA DY005
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Glo Substrate Reagent Pack (Reagent A & B)

ELISA DY993 2
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ELISA Plate-coating Buffer (250 mL of PBS)

ELISA DY006 1
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Reagent Diluent Concentrate 3 (5X, 10 x 21 mL)

ELISA DY004
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Black Polystyrene Microplates, 25 Pack

ELISA DY991 1
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