Human Endoglycan/PODXL2 Antibody Summary
Accession # Q9NZ53
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Endoglycan/PODXL2 in Human HUVEC. Endoglycan/PODXL2 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Goat Anti-Human Endoglycan/PODXL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1524) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell membranes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Endoglycan, also named podocalyxin-like 2 (PODXL2), is a member of the CD34 family of sialomucins. It is a Type I transmembrane protein expressed on endothelial cells, early hematopoietic progenitors and leukocyte subpopulations. Specific endoglycan glycoforms function as L-Selectin ligands.
Citation for Human Endoglycan/PODXL2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Endoglycan, a member of the CD34 family of sialomucins, is a ligand for the vascular selectins.
Authors: Kerr SC, Fieger CB, Snapp KR, Rosen SD
J. Immunol., 2008;181(2):1480-90.
Sample Types: Tissue Homogenates
Applications: Western Blot
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