Human Endoglycan/PODXL2 Antibody

(1 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Endoglycan/PODXL2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human PODXL.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Endoglycan/PODXL2
    Gly33-Thr500
    Accession # Q9NZ53
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human Endoglycan/PODXL2
  • Flow Cytometry
    0.25 µg/106 cells
    HUVEC human umbilical vein endothelial cells
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
Endoglycan/PODXL2 in Human HUVEC. Endoglycan/PODXL2 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Goat Anti-Human Endoglycan/PODXL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1524) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell membranes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Endoglycan/PODXL2
Endoglycan, also named podocalyxin-like 2 (PODXL2), is a member of the CD34 family of sialomucins. It is a Type I transmembrane protein expressed on endothelial cells, early hematopoietic progenitors and leukocyte subpopulations. Specific endoglycan glycoforms function as L-Selectin ligands.
  • Entrez Gene IDs:
    50512 (Human); 319655 (Mouse)
  • Alternate Names:
    Endoglycan; PODLX2; podocalyxin-like 2; podocalyxin-like protein 2; PODXL2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Endoglycan, a member of the CD34 family of sialomucins, is a ligand for the vascular selectins.
    Authors: Kerr SC, Fieger CB, Snapp KR, Rosen SD
    J. Immunol., 2008;181(2):1480-90.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: WB
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