Endoglycan/PODXL2 in Human HUVEC. Endoglycan/PODXL2 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Goat Anti-Human Endoglycan/PODXL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1524) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell membranes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Endoglycan, also named podocalyxin-like 2 (PODXL2), is a member of the CD34 family of sialomucins. It is a Type I transmembrane protein expressed on endothelial cells, early hematopoietic progenitors and leukocyte subpopulations. Specific endoglycan glycoforms function as L-Selectin ligands.
Entrez Gene IDs:
50512 (Human); 319655 (Mouse)
endoglycan; PODLX2; podocalyxin-like 2; podocalyxin-like protein 2; PODXL2
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