Human Endophilin B1/Bif-1 Antibody

Catalog # Availability Size / Price Qty
AF7456
AF7456-SP
Detection of Human Endophilin B1/Bif‑1 by Western Blot.
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Human Endophilin B1/Bif-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Endophilin B1/Bif-1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human SH3GLB2 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Endophilin B1/Bif-1
Ala33-Asn189
Accession # Q9Y371
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
3-15 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Endophilin B1/Bif‑1 by Western Blot. View Larger

Detection of Human Endophilin B1/Bif‑1 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Saos‑2 human osteosarcoma cell line, and U‑118‑MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Endophilin B1/Bif‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7456) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Endophilin B1/Bif‑1 at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Endophilin  B1/Bif‑1 in Jurkat Human Cell Line. View Larger

Endophilin B1/Bif‑1 in Jurkat Human Cell Line. Endophilin B1/Bif‑1 was detected in immersion fixed Jurkat human acute T cell leukemia cell line using Sheep Anti-Human Endophilin B1/Bif‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7456) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry Endophilin  B1/Bif‑1 in Human Pancreatic Cancer Tissue. View Larger

Endophilin B1/Bif‑1 in Human Pancreatic Cancer Tissue. Endophilin B1/Bif‑1 was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using Sheep Anti-Human Endophilin B1/Bif‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7456) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Endophilin B1/Bif-1

Endophilin-B1, also known as SH3GLB1 (Src-Homology 3 Domain-containing GRB2-like protein B1) and BIF-1, is a 40 kDa protein that belongs to the endophilin family of molecules. It is a cytoplasmic and Golgi membrane protein that is expressed in neurons, striated (skeletal and cardiac) muscle cells, and placenta. Endophilin-B1 plays a role in cell homeostasis. In neurons, Endophilin-B1 is phosphorylated by Cdk5, inducing homodimerization and autophagosome formation. In addition, it appears to play a role in the maintenance of mitochondrial integrity. Endophilin-B1 appears to cycle on-and-off the outer mitochondrial membrane (OMM), contributing to OMM integrity. And after the initiation of endocytosis, it also directs EEA1+ TrkA-containing endosomes back into the cell membrane for reuse. Human Endophilin‑B1 is 365 amino acids (aa) in length. It possesses an N-terminal lipid-binding BAR (Bin/Amphiphysin/Rvs) domain (aa 27-261) that contains an internal coiled-coil region, and a C-terminal SH3 domain that binds Pro-rich sequences on potential ligands (309-363). There are at least three potential splice variants for Endophilin-B1. Two are 42 and 43 kDa in size, and possess a 21 and 29 aa insertion after Ser190, respectively. A third potentially utilizes an alternative start site at Met101. Over aa 33-189, human Endophilin-B1 shares 98% aa sequence identity with mouse Endophilin-B1.

Long Name
SH3-domain GRB2-like Endophilin B1/Bax-interacting Factor 1
Entrez Gene IDs
51100 (Human); 54673 (Mouse); 292156 (Rat)
Alternate Names
Bax-interacting factor 1; Bif1; Bif-1; Bif-1dJ612B15.2; CGI-61; Endophilin B1; endophilin-B1; KIAA0491endophilin B1; SH3 domain-containing GRB2-like protein B1; SH3-containing protein SH3GLB1; SH3-domain GRB2-like endophilin B1; SH3-domain, GRB2-like, endophilin B1; SH3GLB1

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Immunohistochemistry Reagents

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