Human EOMES Antibody Summary
Accession # O95936
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human EOMES by Western Blot. Western blot shows lysates of BG01V human embryonic stem cells untreated (-) or mesoendoderm differentiated (+). PVDF Membrane was probed with 1 µg/mL of Human EOMES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6166) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for EOMES at approximately 85-105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
EOMES in mesoderm lineage cells differentiated from BG01V. EOMES was detected in immersion fixed BG01V human embryonic stem cells differentiated into mesoderm using Human EOMES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6166) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of EOMES in Differ-entiated BG01V Human Cells by Flow Cytometry. BG01V human embryonic stem cells differentiated to mesendoderm were stained with Sheep Anti-Human EOMES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6166, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
EOMES (Eomesodermin; Eo from Greek meaning "dawn"/early in mesoderm; also TBR2) is a 72 kDa member of the TBR1 subfamily, T-box family of transcription factors. It is expressed in NK and CD8+ T cells, where CTLA4 activation suppresses EOMES activation of IFN-gamma and granzyme B genes. It is also found in the embryo, where it occurs in forebrain floorplate and migrating neuroblasts at 12.5 weeks gestation. Notably, it is reported to undergo intercellular transfer in fetal Xenopus tissue destined to become mesoderm. Here, it synchronizes a multicellular commitment to a cell lineage. Human EOMES is 686 amino acids (aa) in length. It contains short poly-Ala, -Gly and -Asn motifs, and a DNA-binding T box (aa 276-456). There is one isoform that shows a 19 aa insertion after Ser460. Over aa 471‑686, human EOMES shares 91% aa identity with mouse EOMES.
Citations for Human EOMES Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 6
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Androgens increase excitatory neurogenic potential in human brain organoids
Authors: I Kelava, I Chiaradia, L Pellegrini, AT Kalinka, MA Lancaster
Sample Types: Organoids
The CTNNBIP1-CLSTN1 fusion transcript regulates human neocortical development
Authors: MY Ou, Q Xiao, XC Ju, PM Zeng, J Huang, AL Sheng, ZG Luo
Cell Reports, 2021;35(13):109290.
Sample Types: Organoids
Human microglia states are conserved across experimental models and regulate neural stem cell responses in chimeric organoids
Authors: G Popova, SS Soliman, CN Kim, MG Keefe, KM Hennick, S Jain, T Li, D Tejera, D Shin, BB Chhun, CS McGinnis, M Speir, ZJ Gartner, SB Mehta, M Haeussler, KB Hengen, RR Ransohoff, X Piao, TJ Nowakowski
Cell Stem Cell, 2021;0(0):.
Sample Types: Organoids
A discrete subtype of neural progenitor crucial for cortical folding in the gyrencephalic mammalian brain
Authors: N Matsumoto, S Tanaka, T Horiike, Y Shinmyo, H Kawasaki
Sample Types: Whole Tissue
SARS-CoV-2 Infects the Brain Choroid Plexus and Disrupts the Blood-CSF Barrier in Human Brain Organoids
Authors: L Pellegrini, A Albecka, DL Mallery, MJ Kellner, D Paul, AP Carter, LC James, MA Lancaster
Cell Stem Cell, 2020;0(0):.
Sample Types: Organoid
Reprogramming to pluripotency does not require transition through a primitive streak-like state
Authors: S Raab, M Klingenste, A Möller, A Illing, J Tosic, M Breunig, G Kuales, L Linta, T Seufferlei, SJ Arnold, A Kleger, S Liebau
Sci Rep, 2017;7(1):16543.
Sample Types: Cell Lysates
Applications: Western Blot
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