EOMES (Eomesodermin; Eo from Greek meaning "dawn"/early in mesoderm; also TBR2) is a 72 kDa member of the TBR1 subfamily, T-box family of transcription factors. It is expressed in NK and CD8+ T cells, where CTLA4 activation suppresses EOMES activation of IFN-gamma and granzyme B genes. It is also found in the embryo, where it occurs in forebrain floorplate and migrating neuroblasts at 12.5 weeks gestation. Notably, it is reported to undergo intercellular transfer in fetal Xenopus tissue destined to become mesoderm. Here, it synchronizes a multicellular commitment to a cell lineage. Human EOMES is 686 amino acids (aa) in length. It contains short poly-Ala, -Gly and -Asn motifs, and a DNA-binding T box (aa 276-456). There is one isoform that shows a 19 aa insertion after Ser460. Over aa 471‑686, human EOMES shares 91% aa identity with mouse EOMES.
Human EOMES Antibody
R&D Systems | Catalog # AF6166
Key Product Details
Validated by
Species Reactivity
Validated:
Cited:
Applications
Validated:
Cited:
Label
Antibody Source
Product Specifications
Immunogen
Gly471-Pro686
Accession # O95936
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human EOMES Antibody
Detection of Human EOMES by Western Blot.
Western blot shows lysates of BG01V human embryonic stem cells untreated (-) or mesoendoderm differentiated (+). PVDF Membrane was probed with 1 µg/mL of Human EOMES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6166) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for EOMES at approximately 85-105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
EOMES in mesoderm lineage cells differentiated from BG01V.
EOMES was detected in immersion fixed BG01V human embryonic stem cells differentiated into mesoderm using Human EOMES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6166) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of EOMES in Differ-entiated BG01V Human Cells by Flow Cytometry.
BG01V human embryonic stem cells differentiated to mesendoderm were stained with Sheep Anti-Human EOMES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6166, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Detection of EOMES by Immunohistochemistry
Characterization of the dual reporter using sparse labeling strategy.(A) Schematic representation of sparse labeling in organoid culture using 95% unlabeled control H9 human embryonic stem cells (hESCs) and 5% dual reporter hESCs for embryoid body formation. (B,C) Confocal scan of 40-day-old sparsely labeled organoid sections immunostained with anti-SOX2 (B), anti-NeuN (C), anti-EGFP, and anti-dTomato antibodies. (D) Confocal scan of 60-day-old sparsely labeled organoid sections immunostained with anti-EOMES (BD), anti-EGFP, and anti-dTomato antibodies. Zoomed-in images of the boxed areas are shown on the right. White arrows point at EGFP+ and dTomato+ double positive cells. White triangles point at EOMES and EGFP+ positive cells. The images show a typical ventricular zone and surrounding neuronal zone in the organoid tissue. Scale bar = 100 µm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36989136), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of EOMES by Immunohistochemistry
Characterization of the dual reporter using sparse labeling strategy.(A) Schematic representation of sparse labeling in organoid culture using 95% unlabeled control H9 human embryonic stem cells (hESCs) and 5% dual reporter hESCs for embryoid body formation. (B,C) Confocal scan of 40-day-old sparsely labeled organoid sections immunostained with anti-SOX2 (B), anti-NeuN (C), anti-EGFP, and anti-dTomato antibodies. (D) Confocal scan of 60-day-old sparsely labeled organoid sections immunostained with anti-EOMES (BD), anti-EGFP, and anti-dTomato antibodies. Zoomed-in images of the boxed areas are shown on the right. White arrows point at EGFP+ and dTomato+ double positive cells. White triangles point at EOMES and EGFP+ positive cells. The images show a typical ventricular zone and surrounding neuronal zone in the organoid tissue. Scale bar = 100 µm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36989136), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Human EOMES Antibody
CyTOF-ready
Immunocytochemistry
Sample: Immersion fixed BG01V human embryonic stem cells differentiated into mesoderm
Intracellular Staining by Flow Cytometry
Sample: BG01V human embryonic stem cells differentiated to mesendoderm fixed with paraformaldehyde and permeabilized with saponin
Western Blot
Sample: Mesoendoderm differentiated BG01V human embryonic stem cells
Flow Cytometry Panel Builder
Bio-Techne Knows Flow Cytometry
Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: EOMES
Long Name
Alternate Names
Gene Symbol
UniProt
Additional EOMES Products
Product Documents for Human EOMES Antibody
Product Specific Notices for Human EOMES Antibody
For research use only
Citations for Human EOMES Antibody
Powered by Bioz
Customer Reviews for Human EOMES Antibody
There are currently no reviews for this product. Be the first to review Human EOMES Antibody and earn rewards!
Have you used Human EOMES Antibody?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
