Human EOMES Antibody

Catalog # Availability Size / Price Qty
AF6166
AF6166-SP
Detection of Human EOMES by Western Blot.
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Product Details
Citations (6)
FAQs
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Human EOMES Antibody Summary

Species Reactivity
Human
Specificity
Detects human EOMES in Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human EOMES
Gly471-Pro686
Accession # O95936
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
See below
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human EOMES antibody by Western Blot. View Larger

Detection of Human EOMES by Western Blot. Western blot shows lysates of BG01V human embryonic stem cells untreated (-) or mesoendoderm differentiated (+). PVDF Membrane was probed with 1 µg/mL of Human EOMES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6166) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for EOMES at approximately 85-105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry EOMES antibody in mesoderm lineage cells differentiated from BG01V by Immunocytochemistry (ICC). View Larger

EOMES in mesoderm lineage cells differentiated from BG01V. EOMES was detected in immersion fixed BG01V human embryonic stem cells differentiated into mesoderm using Human EOMES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6166) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Intracellular Staining by Flow Cytometry Detection of EOMES antibody in Differ-entiated BG01V Human Cells antibody by Flow Cytometry. View Larger

Detection of EOMES in Differ-entiated BG01V Human Cells by Flow Cytometry. BG01V human embryonic stem cells differentiated to mesendoderm were stained with Sheep Anti-Human EOMES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6166, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EOMES

EOMES (Eomesodermin; Eo from Greek meaning "dawn"/early in mesoderm; also TBR2) is a 72 kDa member of the TBR1 subfamily, T-box family of transcription factors. It is expressed in NK and CD8+ T cells, where CTLA4 activation suppresses EOMES activation of IFN-gamma and granzyme B genes. It is also found in the embryo, where it occurs in forebrain floorplate and migrating neuroblasts at 12.5 weeks gestation. Notably, it is reported to undergo intercellular transfer in fetal Xenopus tissue destined to become mesoderm. Here, it synchronizes a multicellular commitment to a cell lineage. Human EOMES is 686 amino acids (aa) in length. It contains short poly-Ala, -Gly and -Asn motifs, and a DNA-binding T box (aa 276-456). There is one isoform that shows a 19 aa insertion after Ser460. Over aa 471‑686, human EOMES shares 91% aa identity with mouse EOMES.

Long Name
Eomesodermin Homolog
Entrez Gene IDs
8320 (Human); 13813 (Mouse); 316052 (Rat)
Alternate Names
EOMES; eomesodermin (Xenopus laevis) homolog; Eomesodermin; t box, brain, 2; T-box brain protein 2; T-box brain2; TBR2; TBR-2; TBR2eomesodermin homolog; T-brain-2

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Citations for Human EOMES Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Androgens increase excitatory neurogenic potential in human brain organoids
    Authors: I Kelava, I Chiaradia, L Pellegrini, AT Kalinka, MA Lancaster
    Nature, 2022;0(0):.
    Species: Human
    Sample Types: Organoids
    Applications: IHC
  2. The CTNNBIP1-CLSTN1 fusion transcript regulates human neocortical development
    Authors: MY Ou, Q Xiao, XC Ju, PM Zeng, J Huang, AL Sheng, ZG Luo
    Cell Reports, 2021;35(13):109290.
    Species: Human
    Sample Types: Organoids
    Applications: IHC
  3. Human microglia states are conserved across experimental models and regulate neural stem cell responses in chimeric organoids
    Authors: G Popova, SS Soliman, CN Kim, MG Keefe, KM Hennick, S Jain, T Li, D Tejera, D Shin, BB Chhun, CS McGinnis, M Speir, ZJ Gartner, SB Mehta, M Haeussler, KB Hengen, RR Ransohoff, X Piao, TJ Nowakowski
    Cell Stem Cell, 2021;0(0):.
    Species: Human
    Sample Types: Organoids
    Applications: IHC
  4. A discrete subtype of neural progenitor crucial for cortical folding in the gyrencephalic mammalian brain
    Authors: N Matsumoto, S Tanaka, T Horiike, Y Shinmyo, H Kawasaki
    Elife, 2020;9(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. SARS-CoV-2 Infects the Brain Choroid Plexus and Disrupts the Blood-CSF Barrier in Human Brain Organoids
    Authors: L Pellegrini, A Albecka, DL Mallery, MJ Kellner, D Paul, AP Carter, LC James, MA Lancaster
    Cell Stem Cell, 2020;0(0):.
    Species: Human
    Sample Types: Organoid
    Applications: IHC
  6. Reprogramming to pluripotency does not require transition through a primitive streak-like state
    Authors: S Raab, M Klingenste, A Möller, A Illing, J Tosic, M Breunig, G Kuales, L Linta, T Seufferlei, SJ Arnold, A Kleger, S Liebau
    Sci Rep, 2017;7(1):16543.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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