Epiregulin is a member of the EGF family of growth factors which includes, among others, epidermal growth factor (EGF), transforming growth factor (TGF)-alpha, amphiregulin (ARG), HB (heparin-binding)-EGF, betacellulin, and the various heregulins. All EGF family members are synthesized as transmembrane precursors and are converted to soluble forms by proteolytic cleavage. Epiregulin was originally purified from the mouse fibroblast-derived tumor cell line NIH3T3/T7 (1). The human epiregulin cDNA encodes a 169 amino acid (aa) residues transmembrane precursor with a 29 aa signal peptide, a 21 aa transmembrane domain and a 21 aa cytoplasmic domain. The putative soluble mature Epiregulin comprising the EGF-like domain (aa residues 64‑104) is formed by proteolytic removal of the propeptide regions (2). There is 85% aa sequence homology between human and mouse epiregulins. Epiregulin is expressed primarily in the placenta and macrophages (3). High level expression has also been detected in various carcinomas. Epiregulin specifically binds EGFR (ErbB1) and ErbB4 but not ErbB2 and ErbB3. It activates the homodimers of both ErbB1 and ErbB4. In addition, epiregulin can also activate all possible heteromeric combinations of the four ErbB family members (4). Epiregulin stimulates the proliferation of fibroblasts, smooth muscle cells and hepatocytes. It has been shown to be an autocrine growth factor for epidermal keratinocytes as well as mesangial cells (5, 6). Epiregulin has also been shown to inhibit growth of several epithelial tumor cells. In addition, Epiregulin has been implicated in the implantation process during pregnancy (7).
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Val63-Leu108
Accession # O14944
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human Epiregulin Antibody
Human Epiregulin ELISA Standard Curve.
Recombinant Human Epiregulin protein was serially diluted 2-fold and captured by Rat Anti-Human Epiregulin Monoclonal Antibody (Catalog # MAB14252) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Epiregulin Antigen Affinity-purified Polyclonal Antibody(Catalog # AF1195) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).Applications for Human Epiregulin Antibody
ELISA
This antibody functions as an ELISA capture antibody when paired with Goat Anti-Human Epiregulin Antigen Affinity-purified Polyclonal Antibody(Catalog # AF1195).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Epiregulin DuoSet ELISA Kit (Catalog # DY1195-05) for convenient development of a sandwich ELISA.
Reviewed Applications
Read 1 review rated 5 using MAB14252 in the following applications:
Formulation, Preparation, and Storage
Purification
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Epiregulin
References
- Toyoda, H. et al. (1995) J. Biol. Chem. 270:7495.
- Toyoda, H. et al. (1997) Biochem. J. 326:69.
- Komurasaki, T. et al. (1997) Oncogene 15:2841.
- Shelly, M. et al. (1998) J. Biol. Chem. 273:10496.
- Shirakata, Y. et al. (2000) J. Biol. Chem. 275:5748.
- Mishre, R. et al. (2002) Am. J. Physiol. Renal. Physiol. 283:F1151.
- Das, S.K. et al. (1997) Dev. Biol. 190:178.
Alternate Names
Gene Symbol
UniProt
Additional Epiregulin Products
Product Documents for Human Epiregulin Antibody
Certificate of Analysis
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Product Specific Notices for Human Epiregulin Antibody
For research use only
Related Research Areas
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Application: characterize prior to use. 92%monomer.Sample Tested: Human recombinant antibodySpecies: HumanVerified Customer | Posted 08/15/2025
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- View all Protocols, Troubleshooting, Illustrated assays and Webinars