Human ErbB2/Her2 (Based on Research Grade Trastuzumab Biosimilar) Antibody

R&D Systems | Catalog # MAB9896

Recombinant Monoclonal Antibody For Research Use Only
R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Inhibition of Cell Growth, Flow Cytometry, CyTOF-ready

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # H2M5B
View all ErbB2/Her2 Primary Antibodies »

Product Specifications

Immunogen

Human ErbB2/Her2

Specificity

Detects human ErbB2/Her2 in Western blots. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibodyTrastuzumab and uses a mouse IgG1 backbone structure. This product is for research use only.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images

Detection of ErbB2/Her2 antibody in MCF-7 human cell line antibody by Flow Cytometry.

Detection of ErbB2/Her2 in MCF-7 human cell line by Flow Cytometry.

MCF-7 human cell line was stained with Mouse Anti-Human ErbB2/Her2 Monoclonal Antibody (Catalog # MAB9896, filled histogram) or Mouse IgG1 isotype control (Catalog # MAB002, open histogram) followed by anti-Mouse IgG PE-conjugated Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
Inhibition of Cell Proliferation by ErbB2/Her2 (Based on Research Grade Trastuzumab Biosimilar) Antibody.

Inhibition of Cell Proliferation by ErbB2/Her2 (Based on Research Grade Trastuzumab Biosimilar) Antibody.

Mouse Anti-Human ErbB2/Her2 (Based on Research Grade Trastuzumab Biosimilar) Monoclonal Antibody (Catalog # MAB9896) inhibits proliferation in the SK-BR-3 human breast cancer cell line as measured by Resazurin (Catalog # AR002). The ED50 for this effect is typically 20-100 ng/mL.

Applications

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: MCF-7 human cell line

Inhibition of Cell Growth

Measured by its ability to inhibit proliferation in the SK‑BR‑3 human breast cancer cell line. Brodowicz, T. et al. (1997) Int. J. Cancer 73:875. The ED50 for this effect is typically 20-100 ng/mL.

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ErbB2/Her2

Trastuzumab is a biosimilar directed against the extracellular domain of Her2 (ErbB2). This product uses the variable region sequence of Trastuzumab and has replaced the human IgG1 backbone structure with mouse IgG1.

Long Name

Receptor Tyrosine Protein Kinase ErbB2

Alternate Names

CD340, HER2, Neu Oncogene, NGL, TKR1

Entrez Gene IDs

2064 (Human); 13866 (Mouse); 24337 (Rat); 102146608 (Cynomolgus Monkey)

Gene Symbol

ERBB2

Additional ErbB2/Her2 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices

For research use only

Citations for Human ErbB2/Her2 (Based on Research Grade Trastuzumab Biosimilar) Antibody

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FAQs

Showing  1 - 2 of 2 FAQs Showing All

  • Q: Does this Biosimilar antibody have the same sequence as the therapeutic antibody?

    A: The Biosimilar antibody has the same variable region and constant region sequence as the therapeutic antibody, except for Catalog numbers MAB9677, MAB9575, MAB9577, MAB9589, and their conjugates. These four antibodies have the same variable region as the Biosimilar, but not the same constant region.

  • Q: What is a Biosimilar antibody?

    A: A Biosimilar antibody is a non-therpeutic, recombinantly-expressed antibody which has the same sequence as the corresponding therapeutic antibody.  It is for research use only. The nomenclature of the Biosimilar antibody includes the name of the therapeutic antibody.  For example, Catalog # MAB9577 is called Human EGFR (Research Grade CetuxiMab Biosimilar) antibody.

  • Q: Does this Biosimilar antibody have the same sequence as the therapeutic antibody?

    A: The Biosimilar antibody has the same variable region and constant region sequence as the therapeutic antibody, except for Catalog numbers MAB9677, MAB9575, MAB9577, MAB9589, and their conjugates. These four antibodies have the same variable region as the Biosimilar, but not the same constant region.

  • Q: What is a Biosimilar antibody?

    A: A Biosimilar antibody is a non-therpeutic, recombinantly-expressed antibody which has the same sequence as the corresponding therapeutic antibody.  It is for research use only. The nomenclature of the Biosimilar antibody includes the name of the therapeutic antibody.  For example, Catalog # MAB9577 is called Human EGFR (Research Grade CetuxiMab Biosimilar) antibody.

Showing  1 - 2 of 2 FAQs Showing All
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