Erythropoietin (EPO) a glycoprotein produced primarily by the kidney, is the principal factor that regulates erythropoiesis by stimulating the proliferation and differentiation of erythroid progenitor cells.
Human Erythropoietin/EPO Antibody (9C21D11)
R&D Systems | Catalog # MAB287
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Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Rat, Hamster - Cricetulus (Chinese Hamster)
Applications
Validated:
Neutralization
Cited:
Western Blot, Neutralization, Immunocytochemistry, ELISA Development (Capture)
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 9C21D11
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Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Erythropoietin/EPO
Accession # P01588
Accession # P01588
Specificity
Detects human Erythropoietin/EPO.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human Erythropoietin/EPO Antibody (9C21D11)
Cell Proliferation Induced by Erythropoietin/EPO and Neutralization by Human Erythropoietin/EPO Antibody.
Recombinant Human Erythropoietin/EPO (Tissue Culture Grade) (Catalog # 287-TC) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Erythropoietin/EPO (Tissue Culture Grade) (0.3 units/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Erythropoietin/EPO Monoclonal Antibody (Catalog # MAB287). The ND50 is typically 0.5-1.5 µg/mL.Applications for Human Erythropoietin/EPO Antibody (9C21D11)
Application
Recommended Usage
Neutralization
Measured by its ability to neutralize Erythropoietin/EPO-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.5-1.5 µg/mL in the presence of 0.3 units/mL Recombinant Human Erythropoietin/EPO (Tissue Culture Grade).
Reviewed Applications
Read 2 reviews rated 5 using MAB287 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Erythropoietin/EPO
Alternate Names
ECYT5, EPO, MVCD2
Gene Symbol
EPO
UniProt
Additional Erythropoietin/EPO Products
Product Documents for Human Erythropoietin/EPO Antibody (9C21D11)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Erythropoietin/EPO Antibody (9C21D11)
For research use only
Related Research Areas
Citations for Human Erythropoietin/EPO Antibody (9C21D11)
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Customer Reviews for Human Erythropoietin/EPO Antibody (9C21D11) (2)
5 out of 5
2 Customer Ratings
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Application: ELISASample Tested: mouse serumSpecies: MouseVerified Customer | Posted 04/15/2026human EPO treated mouse developed antibody against human EPOhuman EPO treated mouse developed antibody against human EPO
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Application: Block/NeutralizeSample Tested: Colon cancer cell lineSpecies: HumanVerified Customer | Posted 11/12/2025
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Associated Pathways
