Human Erythropoietin/EPO Antibody

Catalog #: MAB287
5 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB287-SP
MAB287-100
MAB287-500

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Cell Proliferation Induced by Erythropoietin/EPO and Neutralization by Human Erythropoietin/EPO Antibody.

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Citations (5)

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Human Erythropoietin/EPO Antibody Summary

Species Reactivity

Human

Specificity

Detects human Erythropoietin/EPO.

Source

Monoclonal Mouse IgG₁ Clone # 9C21D11

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Erythropoietin/EPO
Accession # P01588

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Neutralization

Measured by its ability to neutralize Erythropoietin/EPO-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND₅₀) is typically 0.5-1.5 µg/mL in the presence of 0.3 units/mL Recombinant Human Erythropoietin/EPO (Tissue Culture Grade).

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cell Proliferation Induced by Erythropoietin/EPO and Neutralization by Human Erythropoietin/EPO Antibody. Recombinant Human Erythropoietin/EPO (Tissue Culture Grade) (Catalog # 287-TC) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Erythropoietin/EPO (Tissue Culture Grade) (0.3 units/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Erythropoietin/EPO Monoclonal Antibody (Catalog # MAB287). The ND₅₀ is typically 0.5-1.5 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Erythropoietin/EPO

Erythropoietin (EPO) a glycoprotein produced primarily by the kidney, is the principal factor that regulates erythropoiesis by stimulating the proliferation and differentiation of erythroid progenitor cells.

Entrez Gene IDs

2056 (Human); 13856 (Mouse); 24335 (Rat)

Alternate Names

ECYT5; EP; EPO; epoetin; Erythropoietin; MGC138142; MVCD2

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human Erythropoietin/EPO Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Continuous Translation of Circularized mRNA Improves Recombinant Protein Titer
Authors: A Costello, NT Lao, N Barron, M Clynes
Metab. Eng., 2019-01-04;0(0):.
Species: Transgenic Hamster
Sample Types: Cell Culture Supernates
Applications: ELISA Development (Capture)
Novel human renal proximal tubular cell line for the production of complex proteins.
Authors: Fliedl L, Manhart G, Kast F, Katinger H, Kunert R, Grillari J, Wieser M, Grillari-Voglauer R
J Biotechnol, 2014-02-16;176(0):29-39.
Species: Human
Sample Types: Recombinant Protein
Applications: ELISA Development (Capture)
Erythropoietin exerts anti-epileptic effects with the suppression of aberrant new cell formation in the dentate gyrus and upregulation of neuropeptide Y in seizure model of rats.
Authors: Kondo A, Shingo T, Yasuhara T, Kuramoto S, Kameda M, Kikuchi Y, Matsui T, Miyoshi Y, Agari T, Borlongan CV, Date I
Brain Res., 2009-08-18;1296(0):127-36.
Species: Rat
Sample Types: In Vivo
Applications: Neutralization
Erythropoietin inhibits respiratory epithelial cell apoptosis in a model of acute lung injury.
Authors: MacRedmond R, Singhera GK, Dorscheid DR
Eur. Respir. J., 2009-01-22;33(6):1403-14.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
Functional and immunochemical characterisation of different antibodies against the erythropoietin receptor.
Authors: Kirkeby A, van Beek J, Nielsen J, Leist M, Helboe L
J Neurosci Methods, 2007-04-12;164(1):50-8.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot