Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 815207
View all ETV1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human ETV1
Asn120-Leu190
Accession # P50549

Specificity

Detects human ETV1 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human ETV1 Antibody

Detection of Human ETV1 antibody by Western Blot.

Detection of Human ETV1 by Western Blot.

Western blot shows lysates of LNCaP human prostate cancer cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ETV1 Monoclonal Antibody (Catalog # MAB9389) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for ETV1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human ETV1 Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: LNCaP human prostate cancer cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ETV1

ETS translocation variant 1 is a protein that in humans is encoded by the ETV1 gene. ETV-1, ETV-4 and ETV-5 proteins form the PEA3 subfamily of ETS transcription factors. Posttranslational modifications in this subfamily promote cell proliferation, motility and invasion.  Disregulation of ETV-1 has been observed in gastrointestinal stromal and protatic tumors, and in triple-negative breast cancer. Human ETV-1 is a 477 aminoacids (aa) protein with at least six known isoforms. Over the immunogen used in this antibody (aa120-190), ETV-1 sequences are 96% identical to mouse and rat ETV-1 sequences.

Long Name

ETS Translocation Variant 1

Alternate Names

ER81

Entrez Gene IDs

2115 (Human); 14009 (Mouse); 362733 (Rat)

Gene Symbol

ETV1

UniProt

P50549

Additional ETV1 Products

Product Documents for Human ETV1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human ETV1 Antibody

For research use only

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