Detects human FABP3 in Western blots. In Western blots, approximately 40% cross-reactivity with recombinant mouse (rm) FABP4 is observed, 20% cross-reactivity with recombinant rat (rr) FABP1 is observed, and 10% cross-reactivity with rrFABP2 and rmFABP5 is observed.
Polyclonal Sheep IgG
E. coli-derived recombinant human FABP3 Met1-Ala133 Accession # P05413
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Recombinant Human FABP3
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
FABP3/H‑FABP in Human Heart. FABP3/H‑FABP was detected in immersion fixed paraffin-embedded sections of human heart using Sheep Anti-Human FABP3/H‑FABP Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1678) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Fatty acid binding proteins are small cytoplasmic lipid binding proteins that are expressed in a tissue specific manner. FABPs bind free fatty acids, cholesterol, and retinoids, and are involved in intracellular lipid transport. Circulating FABP levels are used as indicators of tissue damage. Some FABP polymorphisms have been associated with disorders of lipid metabolism and the development of atherosclerosis.
Human FABP3, also known as FABP-Heart (H-FABP), FABP-Muscle (M-FABP), and mammary derived growth inhibitor (MDGI), is a member of the intracellular FABP family. FABP3 plays a role in the transport of long chain fatty acids in the cytoplasm.
Fatty Acid-Binding Protein 3
Entrez Gene IDs:
FABP11; fatty acid binding protein 11; fatty acid binding protein 3, muscle and heart (mammary-derived growthinhibitor); Fatty acid-binding protein 3; Fatty acid-binding protein 3, muscle; fatty acid-binding protein, heart; Heart-type fatty acid-binding protein; HFABP; H-FABP; H-FABPM-FABP; Mammary-derived growth inhibitor; MDGI; Muscle fatty acid-binding protein; O-FABP
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