FABP3/H-FABP Antibody

R&D Systems | Catalog # AF1678

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Primate - Macaca fuscata (Japanese Macaque)

Applications

Validated:

Immunohistochemistry, Western Blot, Simple Western

Cited:

Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all FABP3/H-FABP Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human FABP3/H‑FABP
Met1-Ala133
Accession # P05413

Specificity

Detects human FABP3/H‑FABP in direct ELISAs and detects human, mouse, and rat FABP3/H-FABP in Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse (rm) FABP4 and recombinant human (rh) FABP4 is observed, approximately 15% cross-reactivity with recombinant rat (rr) FABP1, rhFABP1, rhFABP2, rhFABP7, and rhFABP8 is observed, and less than 10% cross-reactivity with rrFABP2 and rmFABP5 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for FABP3/H-FABP Antibody

Detection of Human, Mouse, and Rat FABP3/H-FABP antibody by Western Blot.

Detection of Human, Mouse, and Rat FABP3/H‑FABP by Western Blot.

Western blot shows lysates of human heart tissue, human skeletal muscle tissue, mouse heart tissue, and rat heart tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human/Mouse/Rat FABP3/H-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1678) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FABP3/H-FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
FABP3/H-FABP antibody in Human Heart by Immunohistochemistry (IHC-P).

FABP3/H‑FABP in Human Heart.

FABP3/H-FABP was detected in immersion fixed paraffin-embedded sections of human heart using Sheep Anti-Human/Mouse/Rat FABP3/H-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1678) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counter-stained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human and Mouse FABP3/H-FABP antibody by Simple WesternTM.

Detection of Human and Mouse FABP3/H‑FABP by Simple WesternTM.

Simple Western lane view shows lysates of human heart tissue and mouse heart tissue, loaded at 0.2 mg/mL. A specific band was detected for FABP3/H-FABP at approximately 19 kDa (as indicated) using 5 µg/mL of Sheep Anti-Human FABP3/H-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1678) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for FABP3/H-FABP Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion-fixed paraffin-embedded sections of human heart and skeleton muscle

Simple Western

5 µg/mL
Sample: Human heart tissue and mouse heart tissue

Western Blot

0.5 µg/mL
Sample: Human heart tissue, human skeletal muscle tissue, mouse heart tissue, and rat heart tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FABP3/H-FABP

Human FABP3, also known as FABP-Heart (H-FABP), FABP-Muscle (M-FABP) and mammary derived growth inhibitor (MDGI), is a member of the intracellular FABP family. It plays a role in the transport of long chain fatty acids in the cytoplasm.

Long Name

Fatty Acid-Binding Protein 3

Alternate Names

H-FABP, HFABP, MDGI

Entrez Gene IDs

2170 (Human)

Gene Symbol

FABP3

UniProt

P05413

Additional FABP3/H-FABP Products

Product Documents for FABP3/H-FABP Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for FABP3/H-FABP Antibody

For research use only

Related Research Areas

Cardiac Stem Cell Markers
Obesity

Citations for FABP3/H-FABP Antibody

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Protocols

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