Key Product Details

Species Reactivity

Human

Applications

Intracellular Staining by Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 466106
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Product Specifications

Immunogen

HEK293 human embryonic kidney cell line transfected with human FATP2
Met1-Leu620
Accession # O14975

Specificity

Detects human FATP2. Stains human FATP2 transfectants but not irrelevant transfectants.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human FATP2 Antibody

Detection of FATP2 antibody in HepG2 Human Cell Line antibody by Flow Cytometry.

Detection of FATP2 in HepG2 Human Cell Line by Flow Cytometry.

HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human FATP2 Mono-clonal Anti-body (Catalog # MAB4659, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Anti-body (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Applications for Human FATP2 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/106 cells
Sample: HepG2 human hepatocellular carcinoma cell line fixed with paraformaldehyde and permeabilized with saponin

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.


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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FATP2

Fatty Acid Transport Protein 2 (FATP2), also known as SLC27A2 (solute carrier family 27, member 2), is a multipass transmembrane protein that participates in the transport and metabolism of long chain fatty acids. It has acyl-CoA synthase activity and contains an AMP-binding motif. FATP2 localizes to the endoplasmic reticulum and is predominantly expressed in the liver and kidney. Human FATP2 shares 82% aa sequence identity with mouse and rat FATP2.

Long Name

Fatty Acid Transport Protein 2

Alternate Names

ACSVL1, FACVL1, SLC27A2, THCA-CoA ligase, VLACS, VLCS

Entrez Gene IDs

11001 (Human); 26458 (Mouse); 65192 (Rat)

Gene Symbol

SLC27A2

UniProt

Additional FATP2 Products

Product Documents for Human FATP2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human FATP2 Antibody

For research use only

Related Research Areas

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