Detection of FCRL3/FcRH3 in Human Blood Lymphocytes by Flow Cytometry.
Human peripheral blood lymphocytes were stained with Goat Anti-Human FCRL3/FcRH3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3126) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108) and Mouse Anti-Human NKp46/NCR1 Phycoerythrin-conjugated Monoclonal Antibody (Catalog # FAB1850P). Quadrant markers were set based on control antibody staining (Catalog # AB-108-C).
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
FCRL3 (Fc Receptor-Like 3), also known as FcRH3, IRTA3, and SPAP2, is a 110 kDa molecule with sequence homology to classical Fc receptors. The type 1 transmembrane FCRL proteins contain from three to nine immunoglobulin-like domains. They are differentially expressed within the B cell lineage and can either promote or inhibit B cell proliferation and activation (1). Mature human FCRL3 consists of a 556 amino acid (aa) extracellular domain (ECD) with six Ig-like domains, a 21 aa transmembrane segment, and a 140 aa cytoplasmic domain with four immunotyrosine inhibitory motifs (ITIMs) (2 - 4). Within the ECD, human and mouse FCRL3 share 35% aa sequence identity. Alternate splicing generates several additional isoforms with deletions or substitutions in both the extracellular and intracellular regions. These include potentially secreted forms that are truncated following the second Ig-like domain (4). FCRL3 is expressed in secondary lymphoid organs on the surface of mature naïve and memory B cells, NK cells, and B cell lines derived from chronic lymphocytic leukemias (2, 3, 5). It is upregulated on B cells following LPS or anti-CD40 stimulation (6). A polymorphism in the FCRL3 promoter induces enhanced transcription and is associated with the development of autoimmune disorders in a Japanese population (6, 7). Tyrosine phosphorylation within the ITIMs of FCRL3 enables its association with SHP-1 (4).
Davis, R.S. (2007) Annu. Rev. Immunol. 25:525.
Miller, I. et al. (2002) Blood, 99:2662.
Davis, R.S. et al. (2001) Proc. Natl. Acad. Sci. 98:9772.
Xu, M.-J. et al. (2002) Biochem. Biophys. Res. Commun. 293:1037.
Polson, A.G. et al. (2006) Int. Immunol. 18:1363.
Kochi, Y. et al. (2005) Nat. Genet. 37:478.
Chistiakov, D.A. and A.P. Chistiakov (2007) Hum. Immunol. 68:375.
Fc Receptor-like 3
Entrez Gene IDs:
115352 (Human); 329693 (Mouse); 680697 (Rat)
CD307c; Fc receptor homolog 3; Fc receptor-like 3; Fc receptor-like protein 3; FcRH3; FCRL3; fcR-like protein 3; hIFGP3; IFGP3; immunoglobulin superfamily receptor translocation associated protein 3; IRTA3; SPAP2
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