Human FCRL6/FcRH6 Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry View Larger

Detection of FCRL6/FcRH6 in Human Peripheral Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human FCRL6/FcRH6 Monoclonal Antibody (Catalog # MAB6745) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) and Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (Catalog # FAB2408P). Quadrant markers were set based on control antibody staining (Catalog # MAB002).

Preparation and Storage

Background: FCRL6/FcRH6

FCRL6 (Fc receptor-like protein 6; also FcRH6 and IFGP6) is a 66 kDa member of the Ig Superfamily.  It is found on CD56dim CD16⁺ NK cells, CD56⁺ CD3⁺ NKT cells, gd T cells, and effector plus effector-memory CD8⁺ T cells.  FCRL6 is not a receptor for immunoglobulin.  It does, however, bind to isoforms of HLA-DR that are expressed on APCs.  Given that FCRL6 contains a cytoplasmic ITIM, FCRL6 may function as an inhibitory receptor for MHC Class II.  Mature human FCRL6 is a 415 amino acid (aa) type I transmembrane protein.  It contains a 288 aa extracellular region (aa 20-307) that shows three C2-type Ig-like domains (aa 20-293), and a 106 aa cytoplasmic domain.  There are multiple potential splice variants.  One shows a deletion of aa 105-200 accompanied by a 13 aa substitution for aa 394-434; a second possesses a 15 aa substitution for aa 383-434; and a third shows an alternative start site seven aa upstream of the standard site accompanied by a 13 aa substitution for aa 394-434.  Over aa 16-312, human FCRL6 shares only 33% aa identity with the mouse counterpart to FCRL6.

Product Datasheets

Related Research Areas

• B Cell Activation
• Fc Receptors
• ITIM/ITAM Immunoreceptors and Related Molecules