Human FGF-23 DuoSet ELISA
Human FGF-23 DuoSet ELISA Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
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Product Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Kit Content
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Reagent Diluent*
Blocking Buffer*
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product
Scientific Data
Product Datasheets
Preparation and Storage
Background: FGF-23
FGF-23 (fibroblast growth factor 23) is one of three FGF family members that appear to play a role in endocrine metabolism. It is described as being a phosphatonin, a molecule that reduces circulating plasma phosphate. FGF-23 does this, in part, by blocking Vitamin D activation. Active Vitamin D promotes intestinal phosphate absorption.
Citations for Human FGF-23 DuoSet ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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Circulating Fibroblast Growth Factor-23 Levels Can Predict Rapid Kidney Function Decline in a Healthy Population: A Community-Based Study
Authors: HY Chen, WC Fang, SC Chu, PH Wang, CC Lee, IW Wu, CY Sun, HJ Hsu, CY Chen, YC Chen, VC Wu, HC Pan
Biomolecules, 2022;13(1):.
Species: Human
Sample Types: Serum
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Genome-wide association study for circulating fibroblast growth factor 21 and 23
Authors: GT Chuang, PH Liu, TW Chyan, CH Huang, YY Huang, CH Lin, JW Lin, CN Hsu, RY Tsai, ML Hsieh, HL Lee, WS Yang, C Robinson-C, CN Hsiung, CY Shen, YC Chang
Sci Rep, 2020;10(1):14578.
Species: Human
Sample Types: Serum
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Fibroblast growth factor 23, endothelium biomarkers and acute kidney injury in critically-ill patients
Authors: FM de Oliveir, CB Araújo, DF de Freitas, BFT Arruda, LJM de Macêdo, VB Salles, GC Meneses, AMC Martins, AB Libório
J Transl Med, 2019;17(1):121.
Species: Human
Sample Types: Plasma
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Circulating Levels of Soluble Klotho and Fibroblast Growth Factor 23 in Mexican Diabetic Patients and Its Association With Early Nephropathy
Authors: A Farías-Bas, HR Martínez-R, EF Gómez-Garc, AM Cueto-Manz, L Cortés-San, LE Hernández-, G Ramírez-Ló, F Mendoza-Ca
Arch. Med. Res., 2019;0(0):.
Species: Human
Sample Types: Serum
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The Value of Older Donors' Klotho Level in Predicting Recipients' Short-Term Renal Function
Authors: G Deng, A Yang, J Wu, J Zhou, S Meng, C Zhu, J Wang, S Shen, J Ma, D Liu
Med. Sci. Monit., 2018;24(0):7936-7943.
Species: Human
Sample Types: Serum
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