Human FGF-23 DuoSet ELISA

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Ancillary Products Available
Human FGF-23 ELISA Standard Curve
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Product Details
Citations (6)
Supplemental Products

Human FGF-23 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
96-well strip plate
Sample Volume Required
100 µL
Assay Range
78.1 - 5,000 pg/mL
Sufficient Materials
For five 96-well plates*
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human FGF-23. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data

Human FGF-23 ELISA Standard Curve

Product Datasheets

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Preparation and Storage

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: FGF-23

FGF-23 (fibroblast growth factor 23) is one of three FGF family members that appear to play a role in endocrine metabolism. It is described as being a phosphatonin, a molecule that reduces circulating plasma phosphate. FGF-23 does this, in part, by blocking Vitamin D activation. Active Vitamin D promotes intestinal phosphate absorption.

Long Name:
Fibroblast Growth Factor 23
Entrez Gene IDs:
8074 (Human); 64654 (Mouse)
Alternate Names:
ADHR; FGF23; FGF-23; fibroblast growth factor 23; HPDR2; HYPF; phosphatonin; PHPTC; tumor-derived hypophosphatemia inducing factor; Tumor-derived hypophosphatemia-inducing factor

Citations for Human FGF-23 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Serum Fibroblast Growth Factor 23 and FGF23 Gene Variants in Patients with Type 2 Diabetes and Essential Hypertension. Association with Chronic Kidney Disease
    Authors: F Mendoza-Ca, A Farías-Bas, EF Gómez-Garc, L Cortés-San, AM Cueto-Manz, LDC Rizo-de la, CA Leal-Corté
    Archives of medical research, 2023-03-10;0(0):.
    Species: Human
    Sample Types: Serum
  2. Circulating Fibroblast Growth Factor-23 Levels Can Predict Rapid Kidney Function Decline in a Healthy Population: A Community-Based Study
    Authors: HY Chen, WC Fang, SC Chu, PH Wang, CC Lee, IW Wu, CY Sun, HJ Hsu, CY Chen, YC Chen, VC Wu, HC Pan
    Biomolecules, 2022-12-24;13(1):.
    Species: Human
    Sample Types: Serum
  3. Genome-wide association study for circulating fibroblast growth factor 21 and 23
    Authors: GT Chuang, PH Liu, TW Chyan, CH Huang, YY Huang, CH Lin, JW Lin, CN Hsu, RY Tsai, ML Hsieh, HL Lee, WS Yang, C Robinson-C, CN Hsiung, CY Shen, YC Chang
    Sci Rep, 2020-09-03;10(1):14578.
    Species: Human
    Sample Types: Serum
  4. Fibroblast growth factor 23, endothelium biomarkers and acute kidney injury in critically-ill patients
    Authors: FM de Oliveir, CB Araújo, DF de Freitas, BFT Arruda, LJM de Macêdo, VB Salles, GC Meneses, AMC Martins, AB Libório
    J Transl Med, 2019-04-11;17(1):121.
    Species: Human
    Sample Types: Plasma
  5. Circulating Levels of Soluble Klotho and Fibroblast Growth Factor 23 in Mexican Diabetic Patients and Its Association With Early Nephropathy
    Authors: A Farías-Bas, HR Martínez-R, EF Gómez-Garc, AM Cueto-Manz, L Cortés-San, LE Hernández-, G Ramírez-Ló, F Mendoza-Ca
    Arch. Med. Res., 2019-02-01;0(0):.
    Species: Human
    Sample Types: Serum
  6. The Value of Older Donors' Klotho Level in Predicting Recipients' Short-Term Renal Function
    Authors: G Deng, A Yang, J Wu, J Zhou, S Meng, C Zhu, J Wang, S Shen, J Ma, D Liu
    Med. Sci. Monit., 2018-11-05;24(0):7936-7943.
    Species: Human
    Sample Types: Serum


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