Human FGF-6 Antibody Summary
Accession # P10767
Human FGF-6 Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Cell Proliferation Induced by FGF‑6 and Neutralization by Human FGF‑6 Antibody. Recombinant Human FGF-6 (Catalog # 238-F6) stimulates proliferation in the the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human FGF-6 (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human FGF-6 Monoclonal Antibody (Catalog # MAB238). The ND50 is typically 1-4 µg/mL in the presence of heparin (1 µg/mL).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Fibroblast Growth Factor-6 (FGF-6), also known as HST-2, is a 25‑28 kDa member of the FGF family of heparin binding polypeptides which are potent regulators of cell proliferation, differentiation, and function. FGF proteins contain a 120 amino acid (aa) core FGF domain that exhibits a beta -trefoil structure (1, 2). Mature human FGF-6 is a 171 aa protein that shares 94% aa sequence identity with mouse and rat FGF-6 (3). It binds and signals primarily through FGF R1c, 2c, and 4 (4). FGF-6 functions as a mitogen for fibroblasts, vascular endothelial cells, and prostate carcinoma cells, and N-linked glycosylation is required for the full mitogenic effect (5‑7). FGF-6 expression is restricted to skeletal muscle during development although it can be upregulated in prostate cancer and Kaposi sarcoma (7‑9). In the adult, FGF-6 is upregulated in injured skeletal muscle and is required for muscle regeneration (10). FGF-6 inhibits the terminal differentiation of myoblasts and also cooperates with TGF-beta 2 to promote chondrogenesis in embryonic somites (8, 11).
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- Asada, M. et al. (1999) Growth Factors 16:293.
- Ropiquet, F. et al. (2000) Cancer Res. 60:4245.
- de Lapeyriere, O. et al. (1993) Development 118:601.
- Li, J. et al. (1993) Cancer 72:2253.
- Floss, T. et al. (1997) Genes Dev. 11:2040.
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Citation for Human FGF-6 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Increased expression of fibroblast growth factor 6 in human prostatic intraepithelial neoplasia and prostate cancer.
Authors: Ropiquet F, Giri D, Kwabi-Addo B, Mansukhani A, Ittmann M
Cancer Res., 2000;60(15):4245-50.
Sample Types: Tissue Homogenates
Applications: ELISA Development
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