Human FGF-6 Antibody

(1 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human FGF-6 in ELISAs. In direct ELISAs, approximately 75% cross-reactivity with recombinant human (rh) FGF-5 and less than 10% cross-reactivity with rhFGF-4 is observed. Does not neutralize the activity of FGF acidic, FGF basic, rhFGF-4, or rhFGF-5.
  • Source
    Monoclonal Mouse IgG1 Clone # 27028
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    E. coli-derived recombinant human FGF-6
    Ala53-Ile208
    Accession # P10767
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
    • Human FGF-6 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Human FGF‑6 Antibody (Catalog # MAB238)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Human FGF‑6 Biotinylated Antibody (Catalog # BAF238)
  • ELISA Standard
     
    Recombinant Human FGF-6 Protein (Catalog # 238-F6)
  • Neutralization
    Measured by its ability to neutralize FGF‑6-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line. Rizzino, A. et al. (1988) Cancer Res. 48:4266. The Neutralization Dose (ND50) is typically 1-4 µg/mL in the presence of 1 ng/mL Recombinant Human FGF‑6 and 1 µg/mL heparin.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Proliferation Induced by FGF‑6 and Neutralization by Human FGF‑6 Antibody. Recombinant Human FGF‑6 (Catalog # 238-F6) stimulates proliferation in the the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human FGF‑6 (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human FGF‑6 Monoclonal Antibody (Catalog # MAB238). The ND50 is typically 1-4 µg/mL in the presence of heparin (1 µg/mL).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FGF-6
Fibroblast Growth Factor-6 (FGF-6), also known as HST-2, is a 25‑28 kDa member of the FGF family of heparin binding polypeptides which are potent regulators of cell proliferation, differentiation, and function. FGF proteins contain a 120 amino acid (aa) core FGF domain that exhibits a beta -trefoil structure (1, 2). Mature human FGF-6 is a 171 aa protein that shares 94% aa sequence identity with mouse and rat FGF-6 (3). It binds and signals primarily through FGF R1c, 2c, and 4 (4). FGF-6 functions as a mitogen for fibroblasts, vascular endothelial cells, and prostate carcinoma cells, and N-linked glycosylation is required for the full mitogenic effect (5‑7). FGF-6 expression is restricted to skeletal muscle during development although it can be upregulated in prostate cancer and Kaposi sarcoma (7‑9). In the adult, FGF-6 is upregulated in injured skeletal muscle and is required for muscle regeneration (10). FGF-6 inhibits the terminal differentiation of myoblasts and also cooperates with TGF-beta 2 to promote chondrogenesis in embryonic somites (8, 11).
  • References:
    1. Wiedlocha, A. and V. Sorensen (2004) Curr. Top. Microbiol. Immunol. 286:45.
    2. Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107.
    3. Marics, I. et al. (1989) Oncogene 4:335.
    4. Ornitz, D.M. et al. (1996) J. Biol. Chem. 271:15292.
    5. Pizette, S. et al. (1991) Cell Growth Differ. 2:561.
    6. Asada, M. et al. (1999) Growth Factors 16:293.
    7. Ropiquet, F. et al. (2000) Cancer Res. 60:4245.
    8. de Lapeyriere, O. et al. (1993) Development 118:601.
    9. Li, J. et al. (1993) Cancer 72:2253.
    10. Floss, T. et al. (1997) Genes Dev. 11:2040.
    11. Grass, S. et al. (1996) Development 122:141.
    12. Long Name:
      Fibroblast Growth Factor 6
    13. Entrez Gene IDs:
      2251 (Human); 14177 (Mouse); 170700 (Rat)
    14. Alternate Names:
      FGF6; FGF-6; fibroblast growth factor 6; HBGF-6; Heparin secretory-transforming protein 2; Heparin-binding growth factor 6; HST2; HST-2; HSTF2; HSTF-2
    Related Research Areas
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    1 Citations: Showing 1 - 1

    1. Increased expression of fibroblast growth factor 6 in human prostatic intraepithelial neoplasia and prostate cancer.
      Authors: Ropiquet F, Giri D, Kwabi-Addo B, Mansukhani A, Ittmann M
      Cancer Res., 2000;60(15):4245-50.
      Species: Human
      Sample Type: Tissue Homogenates
      Application: ELISA Development
    Isotype Controls
    Description Application Cat# Citations Images  

    Mouse IgG1 Isotype Control

    Ctrl MAB002 138  
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    Mouse IgG1 Isotype Control

    Ctrl MAB002R 1
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    Secondary Antibodies
    Description Application Cat# Citations Images  

    Mouse IgG HRP-conjugated Antibody

    WB HAF007 26  
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    Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

    Flow F0102B 10  
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    Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

    Flow, IHC, ICC NL007 9
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    Donkey Anti-Mouse IgG Biotinylated Antibody

    WB BAF018 1
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    Mouse IgG HRP-conjugated Antibody

    WB HAF018 9  
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    Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

    Flow F0101B 2  
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    Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

    Flow, IHC, ICC NL008 8
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    Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

    Flow, IHC, ICC NL009 5
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    Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

    Flow F0103B 5  
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    Goat Anti-Mouse IgG Biotinylated Antibody

    WB BAF007 4
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    Mouse IgG Antibody

    WB AF007 4
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    Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

    IHC VC002  
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    Mouse IgG VisUCyte HRP Polymer Antibody

    IHC VC001  
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    Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

    Flow F0114  
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    Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

    G-202-C 2
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