Detects human FGF-6 in ELISAs. In direct ELISAs, approximately 75% cross-reactivity with recombinant human (rh) FGF-5 and less than 10% cross-reactivity with rhFGF-4 is observed. Does not neutralize the activity of FGF acidic, FGF basic, rhFGF-4, or rhFGF-5.
Monoclonal Mouse IgG1 Clone # 27028
Protein A or G purified from ascites
E. coli-derived recombinant human FGF-6 Ala53-Ile208 Accession # P10767
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Human FGF‑6 Biotinylated Antibody (Catalog # BAF238)
Recombinant Human FGF-6 Protein (Catalog # 238-F6)
Measured by its ability to neutralize FGF‑6-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line. Rizzino, A. et al. (1988) Cancer Res. 48:4266. The Neutralization Dose (ND50) is typically 1-4 µg/mL in the presence of 1 ng/mL Recombinant Human FGF‑6 and 1 µg/mL heparin.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Cell Proliferation Induced by FGF‑6 and Neutralization by Human FGF‑6 Antibody. Recombinant Human FGF‑6 (Catalog # 238-F6) stimulates proliferation in the the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human FGF‑6 (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human FGF‑6 Monoclonal Antibody (Catalog # MAB238). The ND50 is typically 1-4 µg/mL in the presence of heparin (1 µg/mL).
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Fibroblast Growth Factor-6 (FGF-6), also known as HST-2, is a 25‑28 kDa member of the FGF family of heparin binding polypeptides which are potent regulators of cell proliferation, differentiation, and function. FGF proteins contain a 120 amino acid (aa) core FGF domain that exhibits a beta -trefoil structure (1, 2). Mature human FGF-6 is a 171 aa protein that shares 94% aa sequence identity with mouse and rat FGF-6 (3). It binds and signals primarily through FGF R1c, 2c, and 4 (4). FGF-6 functions as a mitogen for fibroblasts, vascular endothelial cells, and prostate carcinoma cells, and N-linked glycosylation is required for the full mitogenic effect (5‑7). FGF-6 expression is restricted to skeletal muscle during development although it can be upregulated in prostate cancer and Kaposi sarcoma (7‑9). In the adult, FGF-6 is upregulated in injured skeletal muscle and is required for muscle regeneration (10). FGF-6 inhibits the terminal differentiation of myoblasts and also cooperates with TGF-beta 2 to promote chondrogenesis in embryonic somites (8, 11).
Wiedlocha, A. and V. Sorensen (2004) Curr. Top. Microbiol. Immunol. 286:45.
Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107.
Marics, I. et al. (1989) Oncogene 4:335.
Ornitz, D.M. et al. (1996) J. Biol. Chem. 271:15292.
Pizette, S. et al. (1991) Cell Growth Differ. 2:561.
Asada, M. et al. (1999) Growth Factors 16:293.
Ropiquet, F. et al. (2000) Cancer Res. 60:4245.
de Lapeyriere, O. et al. (1993) Development 118:601.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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