Human FGF acidic/FGF1 Quantikine ELISA Kit

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DFA00B
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Human FGF acidic ELISA Cell Culture Supernate/Urine Standard Curve
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Product Details
Procedure
Citations (8)
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Supplemental Products
Reviews (2)

Human FGF acidic/FGF1 Quantikine ELISA Kit Summary

Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Urine (50 uL)
Sensitivity
13.9 pg/mL
Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Specificity
Natural and recombinant human FGF acidic
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human FGF acidic Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human FGF acidic in cell culture supernates, serum, plasma, and urine. It contains E. coli-expressed recombinant human FGF acidic and has been shown to accurately quantitate the recombinant factor.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 208 485 766 204 477 761
Standard Deviation 7.6 21.8 22.9 15 29.3 48.7
CV% 3.7 4.5 3 7.4 6.1 6.4

Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 179 441 749 178 433 713
Standard Deviation 8.3 31.8 17.6 15 36.3 61
CV% 4.6 7.2 2.3 8.4 8.4 8.6

Recovery

The recovery of FGF acidic spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 105 93-113
EDTA Plasma (n=4) 102 93-109
Heparin Plasma (n=4) 97 92-110
Serum (n=4) 98 89-108
Urine (n=4) 99 89-108

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of FGF acidic were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human FGF acidic ELISA Linearity

Data Examples

Human FGF acidic ELISA Cell Culture Supernate/Urine Standard Curve

Human FGF acidic ELISA Serum/Plasma Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: FGF acidic/FGF1

FGF acidic, also known as FGF-1, ECGF, and HBGF-1, is a secreted mitogen that stimulates the proliferation of all cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. It plays a number of roles in development, regeneration, and angiogenesis. FGF acidic is released extracellularly as a disulfide-linked homodimer and is stored in complex with extracellular heparan sulfate. The association of FGF acidic with heparan sulfate is a prerequisite for its subsequent interaction with FGF receptors. Internalized FGF acidic can translocate to the cytosol and to the nucleus. Intracellular FGF acidic functions as a survival factor by inhibiting p53 activity and proapoptotic signaling.

Long Name:
Fibroblast Growth Factor acidic
Entrez Gene IDs:
2246 (Human); 14164 (Mouse); 25317 (Rat); 281160 (Bovine)
Alternate Names:
AFGF; alpha; alpha-ECGF; beta-ECGF; ECGF; ECGFB; ECGF-betaAcidic fibroblast growth factor; endothelial cell growth factor, beta; FGF acidic; FGF-1; FGFABeta-endothelial cell growth factor; FGF-alpha; fibroblast growth factor 1 (acidic); GLIO703; HBGF1; HBGF-1; heparin-binding growth factor 1
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 150 µL Assay Diluent
  4.   Add 150 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human FGF acidic/FGF1 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Glioblastoma Cell Resistance to EGFR and MET Inhibition Can Be Overcome via Blockade of FGFR-SPRY2 Bypass Signaling
    Authors: EK Day, NG Sosale, A Xiao, Q Zhong, B Purow, MJ Lazzara
    Cell Rep, 2020;30(10):3383-3396.e7.
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Validation of a Double-Sandwich Enzyme-Linked Immunoassay for Pharmacokinetic Study of an rh-aFGF Hydrogel in Rat Skin and Serum
    Authors: Q Hui, R Yang, C Lu, J Bi, L Li, J Gong, L Zhang, Z Jin, X Li, X Wang
    Front Pharmacol, 2020;11(0):700.
    Species: Human
    Sample Types: Tissue Homogenates
  3. Individuality in FGF1 expression significantly influences platinum resistance and progression-free survival in ovarian cancer.
    Br. J. Cancer, 2012;107(8):1327-36.
    Species: Human
    Sample Types: Cell Culture Supernates
  4. Functional analysis of the chemokine receptor CCR3 on airway epithelial cells.
    Authors: Beck LA, Tancowny B, Brummet ME, Asaki SY, Curry SL, Penno MB, Foster M, Bahl A, Stellato C
    J. Immunol., 2006;177(5):3344-54.
    Species: Human
    Sample Types: Cell Culture Supernates
  5. Humoral and cellular factors responsible for coronary collateral formation.
    Authors: Sherman JA, Hall A, Malenka DJ, De Muinck ED, Simons M
    Am. J. Cardiol., 2006;98(9):1194-7.
    Species: Human
    Sample Types: Plasma
  6. Quantitative assessment of growth factors in reaming aspirate, iliac crest, and platelet preparation.
    Authors: Herrmann S, Green J, Weber T, Scharfenberger A
    Bone, 2006;39(5):1156-63.
    Species: Human
    Sample Types: Tissue Homogenates
  7. Alpha-tocopheryl succinate inhibits malignant mesothelioma by disrupting the fibroblast growth factor autocrine loop: mechanism and the role of oxidative stress.
    Authors: Stapelberg M, Gellert N, Swettenham E, Tomasetti M, Witting PK, Procopio A, Neuzil J
    J. Biol. Chem., 2005;280(27):25369-76.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis.
    Authors: Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H
    J. Immunol., 2005;175(9):6177-89.
    Species: Human
    Sample Types: Cell Culture Supernates

FAQs

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Reviews for Human FGF acidic/FGF1 Quantikine ELISA Kit

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Human FGF acidic Quantikine ELISA Kit
By Anonymous on 06/20/2016
Sample Tested: Muscle tissue

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Human FGF acidic Quantikine ELISA Kit
By Anonymous on 05/09/2016
Sample Tested: bone putty