Human FGFR4 Antibody Summary
Accession # P22455
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human FGF R4 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and Huh-7 human hepatoma cell line. PVDF membrane was probed with 0.2 µg/mL of Rat Anti-Human FGF R4 Monoclonal Antibody (Catalog # MAB6852) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (HAF005). A specific band was detected for FGF R4 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
FGF R4 in Human Pancreas. FGF R4 was detected in immersion fixed paraffin-embedded sections of human pancreas using Rat Anti-Human FGF R4 Monoclonal Antibody (Catalog # MAB6852) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
FGF R4 in MCF‑7 Human Cell Line. FGF R4 was detected in immersion fixed MCF-7 human breast cancer cell line using Rat Anti-Human FGF R4 Monoclonal Antibody (Catalog # MAB6852) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of FGFR4 in HepG2 cells by Flow Cytometry. HepG2 cells were stained with Rat Anti-Human FGFR4 Monoclonal Antibody (Catalog # MAB6852, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Fibroblast growth factors (FGFs) comprise a family of at least eighteen structurally related proteins that are involved in a multitude of physiological and pathological cellular processes, including cell growth, differentiation, angiogenesis, wound healing and tumorgenesis. The biological activities of the FGFs are mediated by a family of type I transmembrane tyrosine kinases which undergo dimerization and autophosphorylation after ligand binding. Four distinct genes encoding closely related FGF receptors, FGF R1‑4, are known. All four genes for FGF Rs encode proteins with an N-terminal signal peptide, three immunoglobulin (Ig)-like domains, an acid-box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine-kinase domain. Multiple forms of FGF R1‑3 are generated by alternative splicing of the mRNAs. A frequent splicing event involving FGF R1 and 2 results in receptors containing all three Ig domains, referred to as the alpha isoform, or only IgII and IgIII, referred to as the beta isoform. Only the alpha isoform has been identified for FGF R3 and FGF R4. Additional splicing events for FGF R1‑3, involving the C-terminal half of the IgIII domain encoded by two mutually exclusive alternative exons, generate FGF receptors with alternative IgIII domains (IIIb and IIIc). A IIIa isoform which is a secreted FGF binding protein containing only the N-terminal half of the IgIII domain plus some intron sequences has also been reported for FGF R1. Mutations in FGF R1‑3 have been found in patients with birth defects involving craniosynostosis. The complex patterns of expression of these receptors as well as the specificity of their interactions with the various FGF ligand family members are under investigation.
- Galzie, Z. et al. (1997) Biochem. Cell Biol. 75:669.
- Burke, D. et al. (1998) Trends Biochem. Sci. 23:59.
Citation for Human FGFR4 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
ONECUT2 facilitates hepatocellular carcinoma metastasis by transcriptionally upregulating FGF2 and ACLY
Authors: D Liu, T Zhang, X Chen, B Zhang, Y Wang, M Xie, X Ji, M Sun, W Huang, L Xia
Cell Death & Disease, 2021;12(12):1113.
Sample Types: Tissue Homogenates
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Human FGFR4 Antibody
Average Rating: 5 (Based on 1 Review)
Have you used Human FGFR4 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image